A PCR detection method for rapid identification of Paenibacillus larvae

Applied and Environmental Microbiology

Volumn 65, Issue 5, 1999, Pages 2243-2245

  Govan, V A ^a   Allsopp, M H ^b   Davison, S ^a  

^a UNIVERSITY OF THE WESTERN CAPE   (South Africa)

^b PLANT PROTECTION RESEARCH INSTITUTE   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

DETECTION METHOD; LARVA; METHODOLOGY;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL SPORE; BACTERIUM DETECTION; HONEYBEE; NONHUMAN; PAENIBACILLUS LARVAE; POLYMERASE CHAIN REACTION;

APIS MELLIFERA; BACTERIA (MICROORGANISMS); INVERTEBRATA; PAENIBACILLUS LARVAE;

EID: 0032924506     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.5.2243-2245.1999     Document Type: Article

References (14)

1. Allipi, A. M. 1995. Detection of Bacillus larvae spores in Argentinian honeys by using a semi-selective medium. Microbiol. Semin. 11:343-350.
2. Ash, C., F. G. Priest, and M. D. Collins. 1993. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks, and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Leeuwenhoek 64:253-260.
3. Avaniss-Aghjani, K., K. Jones, D. Chapman, and C. Brunk. 1994. A molecular technique for identification of bacteria using small subunit rRNA sequences. BioTechniques 17:144-146.
4. Delaplane, K. S. 1991. American foulbrood. Am. Bee J. 131:700-702.
5. Gochnauer, T. A. 1981. The distribution of Bacillus larvae spores in the environs of colonies infected with American foulbrood disease. Am. Bee J. 121:332-335.
6. Hansen, H. 1984. Methods for determining the presence of the foulbrood bacterium Bacillus larvae in honey. Dan. J. Plant. Soil Sci. 88:325-328.
7. Hansen, H. 1984. The incidence of the foulbrood bacterium Bacillus larvae in honeys retailed in Denmark. Dan. J. Plant Soil Sci. 88:329-336.
8. Hansen, H., and B. Rasmussen. 1986. The investigation of honey from bee colonies for Bacillus larvae. Dan. J. Plant Soil Sci. 90:81-86.
9. Hansen, H., B. Rasmussen, and F. Christensen. 1988. A preliminary experiment involving induced infection from Bacillus larvae. Dan. J. Plant Soil Sci. 92:11-15.
10. Hasenian, L. 1961. How long can spores of American foulbrood live? Am. Bee J. 101:298-299.
11. Hornitzky, M. A. Z., and S. Clark. 1991. Culture of Bacillus larvae from bulk honey samples for the detection of American foulbrood. J. Apic. Res. 30: 13-16.
12. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
13. Shimanuki, H., and D. A. Knox. 1988. Improved method for the detection of Bacillus larvae spores in honey. Am. Bee J. 128:353-354.
14. Steinkraus, K. H., and R. A. Morse. 1992. American foulbrood incidence in some US and Canadian honeys. Apidologie 23:497-500.