HER2-targeting recombinant protein with truncated pseudomonas exotoxin A translocation domain efficiently kills breast cancer cells

Cancer Biology and Therapy

Volumn 7, Issue 8, 2008, Pages 1226-1231

  Zhang, Li ^a,b   Zhao, Jing ^a   Wang, Tao ^a   Yu, Cui Juan ^a   Jia, Lin Tao ^a   Duan, Yun You ^b   Yao, Li Bo ^a   Chen, Si Yi ^c   Yang, An Gang ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^b FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^c CENTER FOR CELL AND GENE THERAPY   (United States)

Author keywords

Gene therapy; Granzyme B; HER2; Pseudomonas exotoxin A; Recombinant protein; Translocation domain

Indexed keywords

DNA FRAGMENT; EPIDERMAL GROWTH FACTOR RECEPTOR 2; GRANZYME B; HYBRID PROTEIN; IMMUNOGRB FUSION PROTEIN PSEUDOMONAS EXOTOXIN A [253-358]; IMMUNOGRB FUSION PROTEIN PSEUDOMONAS EXOTOXIN A [275-280]; IMMUNOGRB FUSION PROTEIN PSEUDOMONAS EXOTOXIN A [275-358]; LIPOFECTAMINE; PSEUDOMONAS EXOTOXIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CANCER GENE THERAPY; CELL VIABILITY; CHROMATIN CONDENSATION; CONTROLLED STUDY; CYTOMEGALOVIRUS; EXPRESSION VECTOR; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; MOUSE; NONHUMAN; NUDE MOUSE; PROTEIN EXPRESSION; SK BR 3 CELL; TUMOR GROWTH; TUMOR XENOGRAFT;

EID: 49749150515     PISSN: 15384047     EISSN: 15558576     Source Type: Journal    
DOI: 10.4161/cbt.7.8.6261     Document Type: Article

References (22)

1. Allured VS, Collier RJ, Carroll SF, McKay DB. Proc Natl Acad Sci USA 1986; 83:1320-4.
2. Pastan I, Chaudhary V, FitzGerald DJ. Recombinant toxins as novel therapeutic agents. Annu Rev Biochem 1992; 61:331-54.
3. Ogata M, Chaudhary VK, Pastan I, FitzGerald DJ. Processing of Pseudomonas exotoxin by a cellular protease results in the generation of a 37,000-Da toxin fragment that is translocated to the cytosol. J Biol Chem 1990; 265:20678-85.
4. Ogata M, Fryling CM, Pastan I, FitzGerald DJ. Cell-mediated cleavage of Pseudomonas exotoxin between Arg279 and Gly280 generates the enzymatically active fragment which translocates to the cytosol. J Bio Chem 1992; 267:25396-401.
5. Chiron MF, Fryling CM, FitzGerald DJ. Cleavage of pseudomonas exotoxin and diphtheria toxin by a furin-like enzyme prepared from beef liver. J Biol Chem 1994; 269:18167-76.
6. Moehring JM, Inocencio NM, Robertson BJ, Moehring TJ. Expression of mouse furin in a Chinese hamster cell resistant to Pseudomonas exotoxin A and viruses complements the genetic lesion. J Biol Chem 1993; 268:2590-4.
7. Liao CW, Chen CA, Lee CN, Su YN, Chang MC, Syu MH, Hsieh CY, Cheng WF. Fusion protein vaccine by domains of bacterial exotoxin linked with a tumor antigen generates potent immunologic responses and antitumor effects. Cancer Res 2005; 65:9089-98.
8. Mere J, Morlon-Guyot J, Bonhoure A, Chiche L, Beaumelle B. Acid-triggered membrane insertion of Pseudomonas exotoxin A involves an original mechanism based on pH-regulated tryptophan exposure. J Biol Chem 2005; 280:21194-201.
9. Prior TI, FitzGerald DJ, Pastan I. Translocation mediated by domain II of Pseudomonas exotoxin A: transport of barnase into the cytosol. Biochemistry 1992; 31:3555-9.
10. Taupiac MP, Bebien M, Alami M, Beaumelle B. A deletion within the translocation domain of Pseudomonas exotoxin A enhances translocation efficiency and cytotoxicity concomitantly. Mol Microbiol 1999; 31:1385-93.
11. Kreitman RJ. Chimeric fusion proteins - Pseudomonas exotoxin-based. Curr Opin Investig Drugs 2001; 2:1282-93.
12. Zhao J, Zhang LH, Jia LT, Zhang L, Xu YM, Wang Z, Yu CJ, Peng WD, Wang CJ, Chen SY, Yang AG. Secreted antibody/granzyme B fusion protein stimulstes selective killing of HER-2-overexpressing tumor cells. J Biol Chem 2004; 279:21343-8.
13. Chen SY, Yang AG, Chen JD, Kute T, King CR, Collier J, Cong Y, Yao C, Huang XF. Potent antitumour activity of a new class of tumour-sPEAcific killer cells. Nature 1997; 385:78-80.
14. Chen TY, Hsu CT, Chang KH, Ting CY, Whang-peng J, Hui CF, Hwang J. Development of DNA delivery system using Pseudomonas exotoxin A and a DNA binding region of human DNA topoisomerase I. Appl Microbiol Biotechnol 2000; 53:558-67.
15. Peipp M, Kupers H, Saul D, Schlierf B, Greil J, Zunino SJ, Gramatzki M, Fey GH. A recombinant CD7-specific single-chain immunotoxin is potent inducer of apoptosis in acute leukemic T cells. Cancer Res 2002; 62:2848-55.
16. Chen JR, Liao CW, Mao SJ, Weng CN. A recombinant chimera composed of repeat region RR1 of Mycoplasma hyopneumoniae adhensin with Pseudomonas exotoxin: in vivo evaluation of specific IgG response in mice and pigs. Vet Microbiol 2001; 80:347-57.
17. Hung CF, Cheng WF, Hsu KF, Chai CY, He L, Ling M, Wu TC. Cancer immunotherapy using a DNA vaccine encoding the translocation domain of a bacterial toxin linked to a tumor antigen. Cancer Res 2001; 61:3698-703.
18. McHugh L, Hu S, Lee BK, Santora K, Kennedy PE, Berger EA, Pastan I, Hamer DH. Increased affinity and stability of an anti-HIV-1 envelope immunotoxin by structure-based mutagenesis. J Biol Chem 2002; 277:34383-90.
19. Jia LT, Zhang LH, Yu CJ, Zhao J, Xu YM, Gui JH, Jin M, Ji ZL, Wen WH, Wang CJ, Chen SY, Yang AG. Specific tumoricidal activity of a secreted proapoptotic protein consisting of a HER-2 antibody and constitutively active caspase-3. Cancer Res 2003; 63:3257-62.
20. Xu YM, Wang LF, Jia LT, Qiu XC, Zhao J, Yu CJ, Zhang R, Zhu F, Wang CJ, Jin BQ, Chen SY, Yang AG. A caspase-6 and anti-human epidermal growth factor receptor-2 (HER2) antibody chimeric molecule suppresses the growth of HER2-overexpressing tumors. J Immunol 2004; 173:61-7.
21. Yu CJ, Jia LT, Meng YL, Zhao J, Zhang Y, Qiu XC, Xu YM, Wen WH, Yao LB, Fan DM, Jin BQ, Chen SY, Yang AG. Selective proapoptotic activity of a secreted recombinant antibody/AIF fusion protein in carcinomas overexpressing HER2. Gene Ther 2006; 13:313-20.
22. Voulhoux R, Taupiac MP, Czjzek M, Beaumelle B, Filloux A. Influence of deletions within domain II of exotoxin A on its extracellular secretion from Pseudomonas aeruginosa. J Bacteriol 2000; 182:4051-8.