A simple method for fixation and microdissection of frozen fresh tissue sections for molecular cytogenetic analysis of cancers

Biotechnic and Histochemistry

Volumn 80, Issue 3-4, 2005, Pages 147-156

  Huang, Qiang ^a   Sacks, P G ^b   Mo, J ^b   McCormick, S A ^a   Iacob, C E ^a   Guo, L ^b   Schaefer, S ^a   Schantz, S P ^a  

^a NEW YORK MEDICAL COLLEGE   (United States)

^b NEW YORK UNIVERSITY COLLEGE OF DENTISTRY   (United States)

Author keywords

Biopsy; Comparative genomic hybridization; Degenerate oligonucleotide primed polymerase chain reaction; Frozen fresh sections; H E staining; Microdissection

Indexed keywords

DNA; PROTEINASE K;

ARTICLE; CANCER CELL CULTURE; CANCER TISSUE; CHROMOSOME ANALYSIS; COMPARATIVE GENOMIC HYBRIDIZATION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA EXTRACTION; FROZEN SECTION; HEAD AND NECK CARCINOMA; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MICRODISSECTION; POLYMERASE CHAIN REACTION; RELIABILITY; SENSITIVITY AND SPECIFICITY; SQUAMOUS CELL CARCINOMA; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; TISSUE FIXATION;

BIOPSY; CARCINOMA, SQUAMOUS CELL; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CHROMOSOME ABERRATIONS; CRYOPRESERVATION; CYTOGENETIC ANALYSIS; DNA MUTATIONAL ANALYSIS; DNA, NEOPLASM; HUMANS; MICRODISSECTION; TISSUE CULTURE TECHNIQUES; TISSUE FIXATION;

EID: 29044449861     PISSN: 10520295     EISSN: 14737760     Source Type: Journal    
DOI: 10.1080/10520290500382806     Document Type: Article

References (22)

1. Ahram, M Flaig, MJ Gillespie, JW Duray, PH Linehan, WM Ornstein, DK Niu, S Zhao, Y Petricoin, EF Emmert-Buck, MR. (2003) Evaluation of ethanol fixed, paraffin embedded tissues for proteomic applications Proteomics, 3, pp. 413 - 421.
2. Ben-Ezra, J Johnson, DA Rossi, J Cook, N Wu, A. (1991) Effect of fixation on the amplification of nucleic acids from paraffin embedded material by the polymerase chain reaction J. Histochem. Cytochem., 39, pp. 351 - 354.
3. Craven, RA Banks, RE. (2001) Laser capture microdissection and proteomics: Possibilities and limitations Proteomics, 1, pp. 1200 - 1204.
4. Eltoum, IA Siegal, GP Frost, AR. (2002) Microdissection of histologic sections: Past, present, and future Adv. Anat. Pathol., 9, pp. 316 - 322.
5. Emmert-Buck, MR Bonner, RF Smith, PD Chuaqui, RF Zhuang, Z Goldstein, SR Weiss, RA Liotta, LA. (1996) Laser capture microdissection Science, 274, pp. 998 - 1001.
6. Giercksky, HE Thorstensen, L Qvist, H Nesland, JM Lothe, RA. (1997) Comparison of genetic changes in frozen biopsies and microdissected archival material from the same colorectal liver metastases Diagn. Mol. Pathol., 6, pp. 318 - 325.
7. Hirose, Y Aldape, K Takahashi, M Berger, MS Feuerstein, BG. (2001) Tissue microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) is an effective method to analyze genetic aberrations in invasive tumors J. Mol. Diagn., 3, pp. 62 - 67.
8. Huang, Q Schantz, SP Rao, PH Mo, J McCormick, SA Chaganti, RS. (2000) Improving degenerate oligonucleotide primed PCR-comparative genomic hybridization for analysis of DNA copy number changes in tumors Genes Chromosomes Cancer, 28, pp. 395 - 403.
9. Huang, Q Yu, GP McCormick, SA Mo, J Datta, B Mahimkar, M Lazarus, P Schaffer, AA Desper, R Schantz, SP. (2002) Genetic differences detected by comparative genomic hybridization in head and neck squamous cell carcinomas from different tumor sites: Construction of oncogenetic trees for tumor progression Genes Chromosomes Cancer, 34, pp. 224 - 233.
10. Kallioniemi, A Kallioniemi, OP Sudar, D Rutovitz, D Gray, JW Waldman, F Pinkel, D. (1992) Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors Science, 258, pp. 818 - 821.
11. Kallioniemi, OP Kallioniemi, A Piper, J Isola, J Waldman, FM Gray, JW Pinkel, D. (1994) Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors Genes Chromosomes Cancer, 10, pp. 231 - 243.
12. Lee, JY Dong, SM Kim, SY Yoo, NJ Lee, SH Park, WS. (1998) A simple, precise and economical microdissection technique for analysis of genomic DNA from archival tissue sections Virchows Arch., 433, pp. 305 - 309.
13. Maitra, A Gazdar, AF. (2001) Tissue microdissection and processing Cancer Treat Res., 106, pp. 63 - 84.
14. Moskaluk, CA Kern, SE. (1997) Microdissection and polymerase chain reaction amplification of genomic DNA from histological tissue sections Am. J. Pathol., 150, pp. 1547 - 1552.
15. Shen, KL Yang, LS Hsieh, HF Chen, CJ Yu, JC Tsai, NM Harn, HJ. (2000) Microsatellite alterations on human chromosome 11 in in situ and invasive breast cancer: A microdissection microsatellite analysis and correlation with p53, ER (estrogen receptor), and PR (progesterone receptor) protein immunoreactivity J. Surg. Oncol., 74, pp. 100 - 107.
16. Speicher, MR du Manoir, S Schrock, E Holtgreve-Grez, H Schoell, B Lengauer, C Cremer, T Ried, T. (1993) Molecular cytogenetic analysis of formalin fixed, paraffin embedded solid tumors by comparative genomic hybridization after universal DNA-amplification Hum. Mol. Genet., 2, pp. 1907 - 1914.
17. Su, JM Perlaky, L Li, XN Leung, HC Antalffy, B Armstrong, D Lau, CC. (2004) Comparison of ethanol versus formalin on preservation of histology and RNA in laser capture microdissected brain tissues Brain Pathol., 14, pp. 175 - 182.
18. Telenius, H Carter, NP Bebb, CE Nordenskjold, M Ponder, BA Tunnacliffe, A. (1992) Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer Genomics, 13, pp. 718 - 725.
19. Thompson-Hehir, J Davies, MP Green, JA Halliwell, N Joyce, KA Salisbury, J Sibson, DR Vergote, I Walker, C. (2000) Novel polymerase chain reaction approach for full-coding p53 mutation detection in microdissected archival tumors Diagn. Mol. Pathol., 9, pp. 110 - 119.
20. Weber, RG Scheer, M Born, IA Joos, S Cobbers, JM Hofele, C Reifenberger, G Zoller, JE Lichter, P. (1998) Recurrent chromosomal imbalances detected in biopsy material from oral premalignant and malignant lesions by combined tissue microdissection, universal DNA amplification, and comparative genomic hybridization Am. J. Pathol., 153, pp. 295 - 303.
21. Williams, C Ponten, F Moberg, C Soderkvist, P Uhlen, M Ponten, J Sitbon, G Lundeberg, J. (1999) A high frequency of sequence alterations is due to formalin fixation of archival specimens Am. J. Pathol., 155, pp. 1467 - 1471.
22. Zhuang, Z Bertheau, P Emmert-Buck, MR Liotta, LA Gnarra, J Linehan, WM Lubensky, IA. (1995) A microdissection technique for archival DNA analysis of specific cell populations in lesions < mn in size Am. J. Pathol., 146, pp. 620 - 625.