Improved protocol for PCR detection of mycobacterium leprae in buffered formalin-fixed skin biopsies [2]

International Journal of Leprosy and Other Mycobacterial Diseases

Volumn 72, Issue 2, 2004, Pages 175-178

  Singh, H B ^b   Katoch, V M ^c   Natrajan, M ^c   Sharma, V D ^c   Chauhan, D S ^c   Lavania, Mallika ^c   Sharma, Pragya ^c   Sharma, Mohini ^c   Katoch, K ^a,b,c   Benara, S ^d   Singh, Padam ^c  

^a NATIONAL JALMA INSTITUTE FOR LEPROSY AND OTHER MYCOBACTERIAL DISEASES   (India)

^b Inst Leprosy Mycobacterial Dis   (India)

^c INDIAN COUNCIL OF MEDICAL RESEARCH   (India)

^d Inst for Res in Med Stat (ICMR)

Author keywords

[No Author keywords available]

Indexed keywords

FORMALDEHYDE;

BORDERLINE LEPROSY; CONTROLLED STUDY; GENE AMPLIFICATION; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; INDIA; LEPROMATOUS LEPROSY; LETTER; MAJOR CLINICAL STUDY; MYCOBACTERIUM LEPRAE; NONHUMAN; POLYMERASE CHAIN REACTION; SKIN BIOPSY; TECHNOLOGY; TUBERCULOID LEPROSY;

BIOPSY, NEEDLE; DNA, BACTERIAL; FORMALDEHYDE; HUMANS; LEPROSY; MYCOBACTERIUM LEPRAE; POLYMERASE CHAIN REACTION; SKIN; TISSUE FIXATION;

EID: 3442901053     PISSN: 0148916X     EISSN: None     Source Type: Journal    
DOI: 10.1489/1544-581X(2004)072<0175:IPFPDO>2.0.CO;2     Document Type: Letter

References (11)

1. DE WIT, M. Y. L., FABER, W. R., KRIEG, S. R., DOUGLAS, J. T., LUCAS, S. B., MONTREEWASUVAT, N., PATTYN, S. R., HUSSAIN, R., PONNIGHAUS, J. M., HARTSKEERL, R. A., and KLASTER, P. R. Application of polymerase chain reaction for the detection of Mycobacterium leprae in skin tissues. J. Clin. Microbiol. 29 (1991) 906-910.
2. FIALLO, P., WILLIAMS, D. L., CHAN, G. P., and GILLIS, T. P. Effects of fixation on polymerase chain reaction detection of Mycobacterium leprae. J. Clin. Microbiol. 30 (1992) 3095-3098.
3. HARTSKEERL, R. A., DE WIT, M. Y. L., and KLASTER. P. R. Polymerase chain reaction for the detection of Mycobacterium leprae. J. Gen. Microbiol. 135 (1989) 2355-2364.
4. JAMIL, S., WILSON, S. M., HACKEL, M., HUSSAIN, R., and STOKER, N. G. A colorimetric PCR method for the detection of Mycobacterium leprae in skin biopsies from leprosy patients. Int. J. Lepr. Other Mycobact. Dis. 62 (1994) 512-526.
5. KATOCH, V. M. New investigative techniques in leprosy. In: Dermatology Update. Mumbai: Bhalani Publishing House, 1998, pp. 165-174.
6. MARCHETTI, G., GORI, A., CATOZZI, L., VAGO, L., NEBULONI, M., ROSSI, M. C., ESPOSTI, A. D., BANDEPLA. A., and FRANZETTI, F. Evaluation of PCR in detection of M. tuberculosis from formalin-fixed, paraffin-embedded tissues: Comparison of four amplification assays. J. Clin. Microbiol. 36 (1998) 1512-1517.
7. SHARMA, R. K., KATOCH, K., SHIVANNAVAR, C. T., SHARMA, V. D., NATRAJAN, M., BHATIA, A. S., SAXENA, N., and KATOCH, V. M. Detection of M. leprae by gene amplification combined ethidiumbromide staining and probe hybridization. Int. J. Lepr. Other Mycobact. Dis. 64 (1996) 409-416.
8. SINGH, H. B., KATOCH, K., NATRAJAN, M., SHARMA, R. K., GUPTA, U. D., SHARMA, V. D., SINGH, D., CHAUHAN, D. S., SRIVASTAVA, K., and KATOCH, V. M. Effect of treatment on PCR positivity in multibacillary leprosy patients treated with conventional and newer drugs ofloxacin and minocycline. Acta. Leprol. 11 (1999) 179-182.
9. VAN EMBDEN, J. D. A., CAVE, M. D., CRAWFORD, J. T., DALE, J., EISENACH, K. D., GICQUEL, B., HERMANS MARTIN, C., MEDAM, R., SHINNICK, T. M., and SMALL, P. M. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting recommendations for standardized methodology. J. Clin. Microbiol. 31 (1993) 406-409.
10. WILLIAMS, D. L., GILLIS, T. P., FIALLO, P., JOB, C. K., GELBER, R. H., HILL, C., and IZUMI, S. Detection of Mycobacterium leprae and the potential of monitoring anti-leprosy drug therapy directly from skin biopsies by polymerase chain reaction. Mol. Cell. Probes 6 (1992) 401-410.
11. WOODS, S. A., and COLE, S. T. A rapid method for detection of potentially viable Mycobacterium leprae in human biopsies; a novel application of PCR. FEMS Microbiol. Letts. 65 (1989) 305-310.