Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis

Journal of Clinical Microbiology

Volumn 40, Issue 11, 2002, Pages 4298-4300

  Woodford, Neil ^a   Tysall, Luke ^a   Auckland, Cressida ^a   Stockdale, Mark W ^a   Lawson, Andrew J ^a   Walker, Rachel A ^a   Livermore, David M ^a  

^a CPHL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

OXAZOLIDINONE DERIVATIVE; RIBOSOME DNA;

AMPLICON; ANALYTIC METHOD; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATE; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; GENE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ANTI-BACTERIAL AGENTS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, RIBOSOMAL; DRUG RESISTANCE, BACTERIAL; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; HUMANS; MICROBIAL SENSITIVITY TESTS; MUTATION; OXAZOLIDINONES; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, RIBOSOMAL;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; POSIBACTERIA;

EID: 0036841967     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.11.4298-4300.2002     Document Type: Article

References (11)

1. Cockerill, F. R, III., and T. F. Smith. 2002. Rapid-cycle real-time PCR: A revolution for clinical microbiology. ASM News 68:77-83.
2. Gibson, J. R., N. A. Saunders, B. Burke, and R. J. Owen. 1999. Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori. J. Clin. Microbiol. 37:3746-3748.
3. Jones, R. N., D. J. Biedenbach, and T. R. Anderegg. 2002. In vitro evaluation of AZD2563, a new oxazolidinone, tested against unusual gram-positive species. Diagn. Microbiol. Infect. Dis. 42:119-122.
4. Jones, R. N., P. Della-Latta, L. V. Lee, and D. J. Beidenbach. 2002. Linezolid-resistant Enterococcus faecium isolated from a patient without prior exposure to an oxazolidinone: Report from the SENTRY Antimicrobial Surveillance Program. Diagn. Microbiol. Infect. Dis. 42:137-139.
5. Kloss, P., L. Xiong, D. L. Shinabarger, and A. S. Mankin. 1999. Resistance mutations in 23S rRNA identify the site of action of the protein synthesis inhibitor linezolid in the ribosomal peptidyl transferase center. J. Mol. Biol. 294:93-101.
6. Livermore, D. M. 2000. Quinupristin/dalfopristin and linezolid: Where, when, which and whether to use? J. Antimicrob. Chemother. 46:347-350.
7. Prystowsky, J., F. Siddiqui, J. Chosay, D. L. Shinabarger, J. Millichap, L. R. Peterson, and G. A. Noskin. 2001. Resistance to linezolid: Characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci. Antimicrob. Agents Chemother. 45:2154-2156.
8. Tan, T. Y., S. Corden, R. Barnes, and B. Cookson. 2001. Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR. J. Clin. Microbiol. 39:4529-4531.
9. Tsiodras, S., H. S. Gold, G. Sakoulas, G. M. Eliopoulos, C. Wennersten, L. Venkataraman, R. C. Moellering, and M. J. Ferraro. 2001. Linezolid resistance in a clinical isolate of Staphylococcus aureus. Lancet 358:207-208.
10. Walker, R. A., N. Saunders, A. J. Lawson, E. A. Lindsay, M. Dassama, L. R. Ward, M. J. Woodward, R. H. Davies, E. Liebana, and E. J. Threlfall. 2001. Use of a LightCycler gyrA mutation assay for rapid identification of mutations conferring decreased susceptibility to ciprofloxacin in multiresistant Salmonella enterica serotype Typhimurium DT104 isolates. J. Clin. Microbiol. 39:1443-1448.
11. Woodford, N. 2002. Glycopeptides, streptogramins and oxazolidinones: Long-term solutions or castles in the sand? Contin. Prof. Dev. Infect. 3:4-8.