Amplification of six putative RD1 genes of Mycobacterium tuberculosis for cloning and expression in Escherichia coli and purification of expressed proteins

Medical Principles and Practice

Volumn 17, Issue 5, 2008, Pages 378-384

  Amoudy, Hanady A ^a   Mustafa, Abu S ^a  

^a KUWAIT UNIVERSITY   (Kuwait)

Author keywords

Cloning and expression; Mycobacterium tuberculosis; Protein purification; RD1 genes

Indexed keywords

DNA; HYBRID PROTEIN; PROTEIN ORF11; PROTEIN ORF14; PROTEIN ORF15;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RD1 GENE;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; MYCOBACTERIUM TUBERCULOSIS; OPEN READING FRAMES; RECOMBINANT FUSION PROTEINS;

EID: 49449113504     PISSN: 10117571     EISSN: None     Source Type: Journal    
DOI: 10.1159/000141501     Document Type: Article

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