Detection of a lysozyme inhibitor in Proteus mirabilis by a new reverse zymogram method

Applied and Environmental Microbiology

Volumn 74, Issue 15, 2008, Pages 4978-4981

  Callewaert, Lien ^a   Vanderkelen, Lise ^a   Deckers, Daphne ^a   Aertsen, Abram ^a   Robben, Johan ^b,d   Michiels, Chris W ^a,c  

^a LEUVEN FOOD SCIENCE AND NUTRITION RESEARCH CENTRE LFORCE   (Belgium)

^b HASSELT UNIVERSITY   (Belgium)

^c Laboratory of Food Microbiology   (Belgium)

^d UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; FOOD PRODUCTS;

HEN EGG-WHITE LYSOZYME; LYSOZYME INHIBITOR; PERIPLASMIC PROTEINS; PROTEUS MIRABILIS;

ENZYMES;

ENZYME INHIBITOR; LYSOZYME INHIBITOR; LYSOZYME INHIBITOR IVY;

BACTERIUM; DETECTION METHOD; ENZYME; INHIBITOR; PROTEIN;

ARTICLE; DRUG DETERMINATION; ESCHERICHIA COLI; NONHUMAN; PROTEUS MIRABILIS; ZYMOGRAPHY;

AMINO ACID SEQUENCE; ENZYME INHIBITORS; ENZYME PRECURSORS; MOLECULAR SEQUENCE DATA; MURAMIDASE; OVALBUMIN; PROTEUS MIRABILIS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROTEUS MIRABILIS;

EID: 49449092855     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00219-08     Document Type: Article

References (15)

1. Abergel, C., V. Monchois, D. Byrne, S. Chenivesse, F. Lembo, J.-C. Lazzaroni, and J.-M. Claverie. 2007. Structure and evolution of the Ivy protein family, unexpected lysozyme inhibitors in gram-negative bacteria. Proc. Natl. Acad. Sci. USA 104:6394-6399.
2. Callewaert, L., A. Aertsen, D. Deckers, K. G. A. Vanoirbeek, L. Vanderkelen, J. M. Van Herreweghe, B. Masschalck, D. Nakimbugwe, J. Robben, and C. W. Michiels. 7 March 2008, posting date. A new family of lysozyme inhibitors contributing to lysozyme tolerance in gram-negative bacteria. PLoS Pathog. 4:e1000019. doi:10.1371/journal.ppat.1000019.
3. Callewaert, L., B. Masschalck, D. Deckers, D. Nakimbugwe, M. Atanassova, A. Aertsen, and C. W. Michiels. 2005. Purification of Ivy, a lysozyme inhibitor from Escherichia coli, and characterisation of its specificity for various lysozymes. Enzyme Microb. Technol. 37:205-211.
4. Deckers, D., B. Masschalck, A. Aertsen, L. Callewaert, C. G. M. Van Tiggelen, M. Atanassova, and C. W. Michiels. 2004. Periplasmic lysozyme inhibitor contributes to lysozyme resistance in Escherichia coli. Cell. Mol. Life Sci. 61:1229-1237.
5. Deckers, D., D. Vanlint, L. Callewaert, A. Aertsen, and C. W. Michiels. 2008. Role of the lysozyme inhibitor Ivy in growth or survival of Escherichia coli and Pseudomonas aeruginosa bacteria in hen egg white and in human saliva and breast milk. Appl. Environ. Microbiol. 74:4434-4439.
6. Heukeshoven, J., and R. Dernick. 1988. Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels. Electrophoresis 9:28-32.
7. Ito, Y., A. Yoshikawa, I. Hotani, S. Fukuda, K. Sugimura, and T. Imoto. 1999. Amino acid sequences of lysozymes newly purified from invertebrates imply wide distribution of a novel class in the lysozyme family. Eur. J. Biochem. 259:456-461.
8. Jollès, P. 1996. Lysozymes: model enzymes in biochemistry and biology. Birkhäuser Verlag, Basel, Switzerland.
9. Kaufmann, E., N. Geisler, and K. Weber. 1984. SDS-PAGE strongly over-estimates the molecular masses of the neurofilament proteins. FEBS Lett. 170:81-84.
10. Laemmli, M. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
11. Monchois, V., C. Abergel, J. Sturgis, S. Jeudy, and J.-M. Claverie. 2001. Escherichia coli ykfE ORFan gene encodes a potent inhibitor of c-type lysozyme. J. Biol. Chem. 276:18437-18441.
12. Revington, M., A. Semesi, A. Yee, C. H. Arrowsmith, and G. S. Shaw. 2006. The solution structure of the protein ydhA from Escherichia coli. J. Biomol. NMR 35:295-300.
13. Snoek-van Beurden, P. A. M., and J. W. Von Den Hoff. 2005. Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors. BioTechniques 38:73-83.
14. Tatusov, R. L., N. D. Fedorova, J. D. Jackson, A. R. Jacobs, B. Kiryutin, E. V. Koonin, D. M. Krylov, R. Mazumder, S. L. Mekhedov, A. N. Nikolskaya, B. S. Rao, S. Smirnov, A. V. Sverdlov, S. Vasudevan, Y. I. Wolf, J. J. Yin, and D. A. Natale. 2003. The COG database: an updated version includes eukaryotes. BMC Bioinformatics 4:41.
15. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.