Introduction of cytochrome b mutations in Saccharomyces cerevisiae by a method that allows selection for both functional and non-functional cytochrome b proteins

Biochimica et Biophysica Acta - Bioenergetics

Volumn 1777, Issue 9, 2008, Pages 1147-1156

  Ding, Martina G ^a   Butler, Christine A ^b   Saracco, Scott A ^b   Fox, Thomas D ^b   Godard, François ^c   di Rago, Jean Paul ^c   Trumpower, Bernard L ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

^b Department of Obstetrics Gynecology   (United States)

^c Institut de Biochimie et Genetique Cellulaires   (France)

Author keywords

Biolistic transformation; Cytochrome b; Cytochrome bc1 complex; Mutations; Yeast

Indexed keywords

CYCLOOXYGENASE 2; CYTOCHROME B;

ARG8 GENE; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; GENE; GENE EXPRESSION; MITOCHONDRION; MUTATIONAL ANALYSIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 49349116795     PISSN: 00052728     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbabio.2008.04.029     Document Type: Article

References (28)

1. Ding M.G., di Rago J.-P., and Trumpower B.L. Investigating the Qn site of the cytochrome bc1 complex in Saccharomyces cerevisiae with mutants resistant to ilicicolin H, a novel Qn site inhibitor. J. Biol. Chem. 281 (2006) 36036-36043
2. Bonnefoy N., and Fox T.D. Genetic transformation of Saccharomyces cerevisiae mitochondria. Methods Enzymol. 350 5 (2001) 97-111
3. Okamoto K., Perlman P.S., and Butow R.A. The sorting of mitochondrial DNA and mitochondrial proteins in zygotes: preferential transmission of mitochondrial DNA to the medial bud. J. Cell Biol. 142 (1998) 613-623
4. Dujon B., Slonimski P.P., and Weill L. Mitochondrial genetics. IX. A model for the recombination and segregation of mitochondrial genes in Saccharomyces cerevisiae. Genetics 78 (1974) 415-437
5. Dujon B. The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance (1981), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 505
6. Rak M., Tetaud E., Godard F., Sagot I., Salin B., Duvézin-Caubet S., Slonimski P.P., Rytka J., and di Rago J.-P. Yeast cells lacking the mitochondrial gene encoding the ATP synthase subunit 6 exhibit a selective loss of complex IV and unusual mitochondrial morphology. J. Biol. Chem. 282 (2007) 10853-10864
7. Brasseur G., Saribas A.S., and Daldal F. A compilation of mutations located in the cytochrome b subunit of the bacterial and mitochondrial bc1 complex. Biochim. Biophys. Acta 1275 (1996) 61-69
8. Wong L.-J.C. Pathogenic mitochondrial DNA mutations in protein-coding genes. Muscle Nerve 36 (2007) 279-293
9. Steele D.F., Butler C.A., and Fox T.D. Expression of a recoded nuclear gene inserted into yeast mitochondrial DNA is limited by mRNA-specific translational activation. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 5253-5257
10. Fiumera H.L., Broadley S.A., and Fox T.D. Translocation of mitochondrially synthesized Cox2 domains from the matrix to the intermembrane space. Mol. Cell Biol. 27 (2007) 4664-4673
11. Labouesse M., Netter P., and Schroeder R. Molecular basis of the 'box effect', a maturase deficiency leading to the absence of splicing of two introns located in two split genes of yeast mitochondrial DNA. Eur. J. Biochem. 144 (1984) 85-93
12. Labouesse M. The yeast mitochondrial leucyl-tRNA synthetase is a splicing factor for the excision of several group I introns. Mol. Gen. Genet. 224 (1990) 209-221
13. 1 complex. J. Biol. Chem. 277 (2002) 49397-49402
14. Séraphin B., Boulet A., Simon M., and Faye G. Construction of a yeast strain devoid of mitochondrial introns and its use to screen nuclear genes involved in mitochondrial splicing. Proc. Natl. Acad. Sci U. S. A. 84 (1987) 6810-6814
15. Conde J., and Fink G.R. A mutant of Saccharomyces cerevisiae defective for nuclear fusion. Proc. Natl. Acad. Sci. U. S. A. 73 (1976) 3651-3655
16. Bonnefoy N., Bsat N., and Fox T.D. Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is controlled by the nucleotide sequence specifying the pre-Cox2p leader peptide. Mol. Cell Biol. 21 (2001) 2359-2372
17. Berden J.A., and Slater E.C. The reaction of antimycin with a cytochrome b preparation active in reconstitution of the respiratory chain. Biochim. Biophys. Acta 216 (1970) 237-249
18. 1. J. Biol. Chem. 247 (1972) 1012-1019
19. Fox T.D., Sanford J.C., and McMullin T.W. Plasmids can stably transform yeast mitochondria lacking endogenous mtDNA. Proc. Natl. Acad. Sci. U. S. A. 85 (1988) 7288-7299
20. Hill P., Kessl J., Fisher N., Meshnick S., Trumpower B.L., and Meunier B. Recapitulation in Saccharomyces cerevisiae of cytochrome b mutations conferring resistance to atovaquone in Pneumocystis jiroveci. Antimicrob. Agents Chemother. 47 (2003) 2725-2731
21. Brown T.A., and Trumpower B.L. Strain-dependent variation in carbon source regulation of nucleus-encoded mitochondrial proteins of Saccharomyces cerevisiae. J. Bacteriol. 177 (1995) 1380-1382
22. 1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment. Structure 8 (2000) 669-684
23. di Rago J.-P., and Colson A.-M. Molecular basis for resistance to antimycin and diuron, Q-Cycle inhibitors acting at the Qi site in the mitochondrial ubiquinol-cytochrome c reductase in Saccharomyces cerevisiae. J. Biol. Chem. 263 (1988) 12564-12570
24. di Rago J.-P., Perea J., and Colson A.-M. Isolation and RNA sequence analysis of cytochrome b mutants resistant to funiculosin, a center i inhibitor of the mitochondrial ubiquinol-cytochrome c reductase in Saccharomyces cerevisiae. FEBS Lett. 263 (1990) 93-98
25. 1 complex with a hydroxyquinone anion Qo site inhibitor bound. J. Biol. Chem. 278 (2003) 31303-31311
26. 1 complex and confer anti-malarial drug resistance in Saccharomyces cerevisiae. J. Biol. Chem. 280 (2005) 17142-17148
27. Wenz T., Hellwig P., MacMillan F., Meunier B., and Hunte C. Probing the role of E272 in quinol oxidation of mitochondrial complex III. Biochemistry 45 (2006) 9042-9052
28. 1 complex. J. Biol. Chem. 281 (2006) 30925-30932