Phospho-proteomic approach to identify new targets of leucine deprivation in muscle cells

Analytical Biochemistry

Volumn 381, Issue 1, 2008, Pages 148-150

  Talvas, Jérémie ^a   Obled, Alain ^a   Sayd, Thierry ^b   Chambon, Christophe ^b   Mordier, Sylvie ^a   Fafournoux, Pierre ^a  

^a INRA and University of Auvergne   (France)

^b UR370 QuaPA   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; BISMUTH COMPOUNDS; DESORPTION; ELECTROPHORESIS; MASS SPECTROMETRY; MUSCLE; PHOSPHORYLATION;

EUKARYOTIC TRANSLATION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INITIATION FACTORS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; MOLECULAR CHAPERONES; PHOSPHORYLATED PROTEINS; PROTEOMIC APPROACHES; TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS (2D-PAGE);

PROTEINS;

CHAPERONE; DEAD BOX PROTEIN; GLUCOSE REGULATED PROTEIN; INITIATION FACTOR 4B; LEUCINE; PHOSPHOPROTEIN;

ANIMAL CELL; ARTICLE; CELL STIMULATION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOUSE; MUSCLE CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MALNUTRITION; PROTEIN PHOSPHORYLATION; PROTEOMICS;

EUKARYOTA;

EID: 49249124901     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.05.038     Document Type: Article

References (18)

1. Morandell S., Stasyk T., Grosstessner-Hain K., Roitinger E., Mechtler K., Bonn G.K., and Huber L.A. Phosphoproteomics strategies for the functional analysis of signal transduction. Proteomics 6 (2006) 4047-4056
2. Cohen P. Protein kinases: the major drug targets of the twenty-first century?. Nat. Rev. Drug Discov. 1 (2002) 309-315
3. Reinders J., and Sickmann A. State-of-the-art in phosphoproteomics. Proteomics 5 (2005) 4052-4061
4. Mann M., Ong S.E., Gronborg M., Steen H., Jensen O.N., and Pandey A. Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome. Trends Biotechnol. 20 (2002) 261-268
5. Chen Z., Southwick K., and Thulin C.D. Initial analysis of the phosphoproteome of chinese hamster ovary cells using electrophoresis. J. Biomol. Tech. 15 (2004) 249-256
6. Stasyk T., Morandell S., Bakry R., Feuerstein I., Huck C.W., Stecher G., Bonn G.K., and Huber L.A. Quantitative detection of phosphoproteins by combination of two-dimensional difference gel electrophoresis and phosphospecific fluorescent staining. Electrophoresis 26 (2005) 2850-2854
7. Pandey A., Fernandez M.M., Steen H., Blagoev B., Nielsen M.M., Roche S., Mann M., and Lodish H.F. Identification of a novel immunoreceptor tyrosine-based activation motif-containing molecule, STAM2, by mass spectrometry and its involvement in growth factor and cytokine receptor signaling pathways. J. Biol. Chem. 275 (2000) 38633-38639
8. Maurin A.C., Jousse C., Averous J., Parry L., Bruhat A., Cherasse Y., Zeng H., Zhang Y., Harding H.P., Ron D., and Fafournoux P. The GCN2 kinase biases feeding behavior to maintain amino acid homeostasis in omnivores. Cell Metab. 1 (2005) 273-277
9. Wullschleger S., Loewith R., and Hall M.N. TOR signaling in growth and metabolism. Cell 124 (2006) 471-484
10. Talvas J., Obled A., Fafournoux P., and Mordier S. Regulation of protein synthesis by leucine starvation involves distinct mechanisms in mouse C2C12 myoblasts and myotubes. J. Nutr. 136 (2006) 1466-1471
11. Yan J.X., Wait R., Berkelman T., Harry R.A., Westbrook J.A., Wheeler C.H., and Dunn M.J. A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. Electrophoresis 21 (2000) 3666-3672
12. Neuhoff V., Arold N., Taube D., and Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9 (1988) 255-262
13. Guy G.R., Philip R., and Tan Y.H. Analysis of cellular phosphoproteins by two-dimensional gel electrophoresis: applications for cell signaling in normal and cancer cells. Electrophoresis 15 (1994) 417-440
14. Wang J.Y., Chambon C., Lu C.D., Huang K.W., Vivares C.P., and Texier C. A proteomic-based approach for the characterization of some major structural proteins involved in host-parasite relationships from the silkworm parasite Nosema bombycis (Microsporidia). Proteomics 7 (2007) 1461-1472
15. Ni M., and Lee A.S. ER chaperones in mammalian development and human diseases. FEBS Lett. 581 (2007) 3641-3651
16. Freiden P.J., Gaut J.R., and Hendershot L.M. Interconversion of three differentially modified and assembled forms of BiP. EMBO J. 11 (1992) 63-70
17. Raught B., Peiretti F., Gingras A.C., Livingstone M., Shahbazian D., Mayeur G.L., Polakiewicz R.D., Sonenberg N., and Hershey J.W. Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases. EMBO J. 23 (2004) 1761-1769
18. Gingras A.C., Raught B., and Sonenberg N. eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation. Annu. Rev. Biochem. 68 (1999) 913-963