Quantitative detection of phosphoproteins by combination of two-dimensional difference gel electrophoresis and phosphospecific fluorescent staining

Electrophoresis

Volumn 26, Issue 14, 2005, Pages 2850-2854

  Stasyk, Taras ^a   Morandell, Sandra ^a   Bakry, Rania ^b   Feuerstein, Isabel ^b   Huck, Christian W ^b   Stecher, Guenther ^b   Bonn, Guenther K ^b   Huber, Lukas A ^a  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

^b UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

Fluorescence; Phosphoproteome; Proteomics; Two dimensional difference gel electrophoresis

Indexed keywords

ELECTROPHORESIS; GELS; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PROTEINS;

EPIDERMAL GROWTH FACTORS; FLUORESCENCE DETECTION; FLUORESCENT STAINING; MAMMARY EPITHELIAL CELLS; PHOSPHOPROTEOMES; PHOSPHORYLATED PROTEINS; PROTEOMICS; QUANTITATIVE DETECTION; SUBCELLULAR FRACTIONATION; TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS;

FLUORESCENCE;

CYTOKERATIN; EPIDERMAL GROWTH FACTOR; FLUORESCENT DYE; MITOGEN ACTIVATED PROTEIN KINASE; PHOSPHOPROTEIN;

ACCURACY; ANIMAL CELL; ARTICLE; BREAST EPITHELIUM; CELL FRACTIONATION; DIFFERENCE GEL ELECTROPHORESIS; ENDOSOME; FLUORESCENCE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEOMICS; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 23144451918     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200500026     Document Type: Article

References (11)

1. Görg, A., Weiss, W., Dunn, M. J., Proteomics 2004, 4, 3665-3685.
2. Stasyk, T., Huber, L. A., Proteomics 2004, 4, 3704-3716.
3. Patton, W. F., Beechem, J. M., Curr. Opin. Chem. Biol. 2002, 6, 63-69.
4. Ünlü, M., Morgan, M. E., Minden, J. S., Electrophoresis 1997, 18, 2071-2077.
5. Berggren, K. N., Schulenberg, B., Lopez, M. F., Steinberg, T. H., et al., Proteomics 2002, 2, 486-498.
6. Rabilloud, T., Strub, J. M., Luche, S., van Dorsselaer, A., Lunardi, J., Proteomics 2001, 1, 699-704.
7. Lamanda, A., Zahn, A., Roder, D., Langen, H., Proteomics 2004, 4, 599-608.
8. Steinberg, T. H., Agnew, B. J., Gee, K. R., Leung, W. Y., et al., Proteomics 2003, 3, 1128-1144.
9. Fialka, I., Pasquali, C., Lottspeich, F., Ahorn, H., Huber, L. A., Electrophoresis 1997, 18, 2582-2590.
10. Takihara, Y., Matsuda, Y., Irie, K., Matsumoto, K., Hara, J., Exp. Cell Res. 2000, 260, 96-104.
11. Stensballe, A., Jensen, O. N., Rapid Commun. Mass Spectrom. 2004, 18, 1721-1730.