Conformation and side chains environments of recombinant human lnterleukin-1 receptor antagonist (rh-IL-1ra) probed by Raman, Raman optical activity, and UV-resonance Raman spectroscopy

Journal of Pharmaceutical Sciences

Volumn 97, Issue 6, 2008, Pages 2228-2241

  Wen, Zai Qing ^a   Cao, Xiaolin ^a   Vance, Aylin ^a  

^a AMGEN INC   (United States)

Author keywords

Conformation; Free sulfhydryl; Protein structure; Raman; Raman optical activity; rh IL lra; Side chain structure; Tryptophan; UV resonance Raman spectroscopy

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); CATION; CYSTEINE; INTERLEUKIN 1 RECEPTOR BLOCKING AGENT; RECOMBINANT HUMAN INTERLEUKIN 1 RECEPTOR ANTAGONIST; TRYPTOPHAN; UNCLASSIFIED DRUG; AROMATIC AMINO ACID; DISULFIDE; IL1RN PROTEIN, HUMAN; RECOMBINANT PROTEIN;

ARTICLE; CONFORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DISULFIDE BOND; HYDROGEN BOND; MOLECULAR INTERACTION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; OPTICAL ROTATION; RAMAN SPECTROMETRY; STRUCTURE ANALYSIS; TITRIMETRY; ULTRAVIOLET SPECTROSCOPY; X RAY DIFFRACTION; CHEMICAL STRUCTURE; CHEMISTRY; CRYSTALLIZATION; HUMAN; METHODOLOGY; PHARMACEUTICS; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; SOLUTION AND SOLUBILITY; ULTRAVIOLET SPECTROPHOTOMETRY;

AMINO ACIDS, AROMATIC; CRYSTALLIZATION; CYSTEINE; DISULFIDES; HUMANS; HYDROGEN BONDING; INTERLEUKIN 1 RECEPTOR ANTAGONIST PROTEIN; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SOLUTIONS; SPECTROPHOTOMETRY, ULTRAVIOLET; SPECTRUM ANALYSIS, RAMAN; TECHNOLOGY, PHARMACEUTICAL;

EID: 49249123111     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.21191     Document Type: Article

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