A novel method for determination of the affinity of protein: Protein interactions in homogeneous assays

Journal of Biomolecular Screening

Volumn 13, Issue 7, 2008, Pages 674-682

  Newton, Philip ^a   Harrison, Paula ^a   Clulow, Stephen ^a  

^a MEDIMMUNE   (United States)

Author keywords

Affinity measurement; Assay development; Cheng Prusoff; Homogeneous time resolved fluorescence (HTRF )

Indexed keywords

BUFFER; CD134 ANTIGEN; OX40 LIGAND; RADIOLIGAND; REAGENT; RECOMBINANT RECEPTOR; TRACER; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND RECEPTOR; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND RECEPTOR 4;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; BINDING SITE; BIOTINYLATION; COMPARATIVE STUDY; COMPETITIVE INHIBITION; CONCENTRATION RESPONSE; DRUG SCREENING; FLUORESCENCE; HIGH THROUGHPUT SCREENING; HOMOGENEOUS TIME RESOLVED FLUORESCENCE; IC 50; LIGAND BINDING; LINEAR SYSTEM; MATHEMATICAL COMPUTING; MATHEMATICAL MODEL; MOLECULAR INTERACTION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING;

BIOTIN; BIOTINYLATION; BUFFERS; DRUG EVALUATION, PRECLINICAL; HUMANS; INHIBITORY CONCENTRATION 50; KINETICS; LIGANDS; OX40 LIGAND; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEINS; RECEPTORS, TNF-RELATED APOPTOSIS-INDUCING LIGAND; RECOMBINANT PROTEINS; TNF-RELATED APOPTOSIS-INDUCING LIGAND;

EID: 49249106287     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057108321086     Document Type: Article

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