Quadruplex real-time PCR assay using allele-specific scorpion primers for detection of mutations conferring clarithromycin resistance to Helicobacter pylori

Journal of Clinical Microbiology

Volumn 46, Issue 7, 2008, Pages 2320-2326

  Burucoa, Christophe ^a,b   Garnier, Martine ^a,b   Silvain, Christine ^a,b   Fauchère, Jean Louis ^a,b  

^a UNIVERSITÉ DE POITIERS   (France)

^b POITIERS UNIVERSITY HOSPITAL   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CLARITHROMYCIN; RNA 23S; ANTIINFECTIVE AGENT; PRIMER DNA;

ACCURACY; ALLELISM; ANALYTIC METHOD; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOPSY; CONTROLLED STUDY; DEVICE; EPSILOMETER TEST; FLUORESCENCE; GENE MUTATION; GENE SEQUENCE; HELICOBACTER INFECTION; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SCORPION; SENSITIVITY AND SPECIFICITY; SINGLE NUCLEOTIDE POLYMORPHISM; WILD TYPE; ALLELE; COMPARATIVE STUDY; DRUG EFFECT; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MISSENSE MUTATION; POLYMERASE CHAIN REACTION; STOMACH MUCOSA; VALIDATION STUDY;

HELICOBACTER PYLORI; SCORPIONES;

ALLELES; ANTI-BACTERIAL AGENTS; BIOPSY; CLARITHROMYCIN; DNA PRIMERS; DRUG RESISTANCE, BACTERIAL; FLUORESCENCE; GASTRIC MUCOSA; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; MICROBIAL SENSITIVITY TESTS; MUTATION, MISSENSE; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SENSITIVITY AND SPECIFICITY;

EID: 49049113736     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.02352-07     Document Type: Article

References (27)

1. Alm, R. A., L. S. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. Dejonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nackelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jiang, D. E. Taylor, G. F. Vovis, and T. J. Trust. 1999. Genomic sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397:176-180.
2. Burucoa, C., C. Landron, M. Garnier, J. L. Fauchère, R. Khan, and M. Rahman. 2005. T2182C mutation is not associated with clarithromycin resistance in Helicobacter pylori. Antimicrob. Agents Chemother. 49:868-870. (Letter.)
3. Chisholm, S. A., R. Owen, E. L. Teare, and S. Saverymuttu. 2001. PCR-based diagnosis of Helicobacter pylori infection and real-time determination of clarithromycin resistance directly from human gastric biopsy samples. J. Clin. Microbiol. 39:1217-1220.
4. Dore, M. P., G. Leandro, G. Realdi, A. R. Sepulveda, and D. Y. Graham. 2000. Effect of pre-treatment antibiotic resistance to metronidazole and clarithromycin on outcome of Helicobacter pylori therapy: a meta-analytical approach. Dig. Dis. Sci. 45:68-76.
5. Dunn, B. E., H. Cohen, and M. J. Blaser. 1997. Helicobacter pylori. Clin. Microbiol. Rev. 10:720-741.
6. Elviss, N. C., A. J. Lawson, and R. J. Owen. 2004. Application of 3′-mismatched reverse primer PCR compared with real-time PCR and PCR-RFLP for the rapid detection of 23S rDNA mutations associated with clarithromycin resistance in Helicobacter pylori. Int. J. Antimicrob. Agents 23:349-355.
7. Fontana, C., M. Favaro, S. Minelli, A. A. Criscuolo, A. Pietroiusti, A. Galante, and C. Favalli. 2002. New site of modification of 23S rRNA associated with clarithromycin resistance of Helicobacter pylori clinical isolates. Antimicrob. Agents Chemother. 46:3765-3769.
8. Gibson, J. R., N. A. Saunders, B. Burke, and R. J. Owen. 1999. Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori. J. Clin. Microbiol. 37:3746-3748.
9. Grignon, B., J. Tankovic, F. Megraud, Y. Glupczynski, M. O. Husson, M. C. Conroy, J. P. Emond, J. Loudergue, J. Raymond, and J. L. Fauchere. 2002. Validation of diffusion methods for macrolide susceptibility testing of Helicobacter pylori. Microb. Drug Resist. 8:61-66.
10. Houben, M. H., D. van de Beek, E. F. Hensen, A. J. Craen, E. A. Rauws, and G. N. Tygat. 1999. A systematic review of Helicobacter pylori eradication therapy: the impact of antimicrobial resistance on eradication rate. Aliment. Pharmacol. Ther. 13:1047-1055.
11. Hultén, K., A. Gibreel, O. Sköld, and L. Engstrand. 1997. Macrolide resistance in Helicobacter pylori: mechanism and stability in strains from clarithromycin-treated patients. Antimicrob. Agents Chemother. 41:2550-2553.
12. Lascols, C., D. Lamarque, J.-M. Costa, C. Copie-Bergman, J.-M. Le Glaunec, L. Deforges, C.-J. Soussy, J.-C. Petit, J.-C. Delchier, and J. Tankovic. 2003. Fast and accurate quantitative detection of Helicobacter pylori and identification of clarithromycin resistance mutations in H. pylori isolates from gastric biopsy specimens by real-time PCR. J. Clin. Microbiol. 41:4573-4577.
13. Malfertheiner, P., F. Megraud, C. O'Morain, F. Bazzoli, E. El-Omar, D. Graham, R. Hunt, T. Rokkas, N. Vakil, and E. J. Kuipers. 14 December 2006, posting date. Current concepts in the management of Helicobacter pylori infection. The Maastricht III Consensus Report. Gut. doi:10.1136/gut.2006. 101634.
14. Matsumura, M., Y. Hikiba, K. Ogura, G. Togo, I. Tsukuda, K. Ushikawa, Y. Shiratori, and M. Omata. 2001. Rapid detection of mutations in the 23S rRNA gene of Helicobacter pylori that confers resistance to clarithromycin treatment to the bacterium. J. Clin. Microbiol. 39:691-695.
15. Megraud, F. 2004. H. pylori antibiotic resistance: prevalence, importance and advantages in testing. Gut 53:1374-1384.
16. Megraud, F., and P. Lehours. 2007. Helicobacter pylori detection and antimicrobial susceptibility testing. Clin. Microbiol. Rev. 20:280-322.
17. Ng, C. T., C. A. Gilchrist, A. Lane, S. Roy, R. Haque, and E. R. Houpt. 2005. Multiplex real-time PCR assay using Scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples. J. Clin. Microbiol. 43:1256-1260.
18. Occhialini, A., M. Urdaci, F. Doucet-Populaire, C. M. Bébéar, H. Lamouliatte, and F. Mégraud. 1997. Macrolide resistance in Helicobacter pylori: rapid detection of point mutations and assays of macrolide binding to ribosomes. Antimicrob. Agents Chemother. 41:2724-2728.
19. Oleastro, M., A. Ménard, A. Santos, H. Lamouliatte, L. Monteiro, P. Barthélémy, and F. Mégraud. 2003. Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori. J. Clin. Microbiol. 41:397-402.
20. Raymond, J., C. Burucoa, O. Pietrini, M. Bergeret, A. Decoster, A. Wann, C. Dupont, and N. Kalach. 2007. Prevalence and coexistence of different mutations conferring clarithromycin resistance in Helicobacter pylori strains isolated from French children. Helicobacter 12:157-163.
21. Schabereiter-Gurtner, C., A. M. Hirschl, B. Dragosics, P. Hufnagl, S. Puz, Z. Kovách, M. Rotter, and A. Makristathis. 2004. Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens. J. Clin. Microbiol. 42:4512-4518.
22. Taylor, D. E., Z. Ge, D. Purych, T. Lo, and K. Hiratsuka. 1997. Cloning and sequence analysis of two copies of a 23S rRNA gene from Helicobacter pylori and association of clarithromycin resistance with 23S rRNA mutations. Antimicrob. Agents Chemother. 41:2621-2628.
23. Trebesius, K., K. Panthel, S. Strobel, K. Vogt, G. Faller, T. Kirchner, M. Kist, J. Heesemann, and R. Haas. 2000. Rapid and specific detection of Helicobacter pylori macrolide resistance in gastric tissue by fluorescent in situ hybridisation. Gut 46:608-614.
24. van Doorn, L.-J., Y. Glupzynski, J. G. Kusters, F. Mégraud, P. Midolo, N. Maggi-Solcà, D. M. M. Queiroz, N. Nouhan, E. Stet, and W. G. V. Quint. 2001. Accurate prediction of macrolide resistance in Helicobacter pylori by a PCR line probe assay for detection of mutations in the 23S rRNA gene: multicenter validation study. Antimicrob. Agents Chemother. 45:1500-1504.
25. Versalovic, J., D. Shortridge, K. Kibler, M. V. Griffy, J. Beyer, R. K. Flamm, S. K. Tanaka, D. Y. Graham, and M. F. Go. 1996. Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori. Antimicrob. Agents Chemother. 40:477-480.
26. Whitcombe, D., J. Theaker, S. P. Guy, T. Brown, and S. Little. 1999. Detection of PCR products using self-probing amplicons and fluorescence. Nat. Biotechnol. 17:804-807.
27. Xia, Q. F., S. X. Xu, D. S. Wang, Y. A. Wen, X. Qin, S. Y. Qian, Z. L. Zhan, H. M. Wang, Y. Z. Lin, and Z. G. Tu. 12 January 2007, posting date. Development of a novel quantitative real-time assay using duplex scorpion primer for detection of Chlamydia trachomatis. Exp. Mol. Pathol. doi:10.1016/j.yexmp.2006.11.005.