Highly efficient and low-cost method to isolate human blood monocytes with high purity

Journal of Immunological Methods

Volumn 337, Issue 1, 2008, Pages 78-80

  Stögbauer, Fabian ^a   Neumeier, Markus ^a   Weigert, Johanna ^a   Wanninger, Josef ^a   Grandl, Margot ^a   Lehle, Karla ^a   Schmitz, Gerd ^a   Aslanidis, Charalampos ^a   Schäffler, Andreas ^a   Schölmerich, Jürgen ^a   Buechler, Christa ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Blood monocytes; CD14 MicroBeads; Purity

Indexed keywords

CD14 ANTIGEN;

ARTICLE; CELL DIFFERENTIATION; CELL ISOLATION; CELL POPULATION; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MONOCYTE; PRIORITY JOURNAL;

ADULT; ANTIGENS, CD14; FEMALE; FLOW CYTOMETRY; HUMANS; IMMUNOMAGNETIC SEPARATION; MALE; MICROSPHERES; MONOCYTES;

EID: 48949115628     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.05.008     Document Type: Article

References (7)

1. Bernardo J., Billingslea A.M., Ortiz M.F., Seetoo K.F., Macauley J., and Simons E.R. Adherence-dependent calcium signaling in monocytes: induction of a CD14-high phenotype, stimulus-responsive subpopulation. J. Immunol. Methods. 209 (1997) 165
2. Buechler C., Ritter M., Quoc C.D., Agildere A., and Schmitz G. Lipopolysaccharide inhibits the expression of the scavenger receptor Cla-1 in human monocytes and macrophages. Biochem. Biophys. Res. Commun. 262 (1999) 251
3. Buechler C., Ullrich H., Ritter M., Porsch-Oezcueruemez M., Lackner K.J., Barlage S., Friedrich S.O., Kostner G.M., and Schmitz G. Lipoprotein (a) up-regulates the expression of the plasminogen activator inhibitor 2 in human blood monocytes. Blood 97 (2001) 981
4. Kaplan M.J., Lewis E.E., Shelden E.A., Somers E., Pavlic R., McCune W.J., and Richardson B.C. The apoptotic ligands TRAIL, TWEAK, and Fas ligand mediate monocyte death induced by autologous lupus T cells. J. Immunol. 169 (2002) 6020
5. Kehrl J.H. Hematopoietic lineage commitment: role of transcription factors. Stem. Cells. 13 (1995) 223
6. Repnik U., Knezevic M., and Jeras M. Simple and cost-effective isolation of monocytes from buffy coats. J. Immunol. Methods. 278 (2003) 283
7. Seta N., and Kuwana M. Human circulating monocytes as multipotential progenitors, Keio. J. Med. 56 (2007) 41