Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii

Brazilian Journal of Microbiology

Volumn 39, Issue 2, 2008, Pages 314-320

  Zhu, Yanping ^a   Pan, Jieru ^a   Qiu, Junzhi ^a   Guan, Xiong ^a  

^a FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY   (China)

Author keywords

Chitinase; Cloning; Homology modeling; Overexpression; Verticillium lecanii

Indexed keywords

ALEYRODIDAE; APHIDIDAE; ESCHERICHIA COLI; FUNGI; HEXAPODA; TORRUBIELLA CONFRAGOSA;

EID: 48849108207     PISSN: 15178382     EISSN: 16784405     Source Type: Journal    
DOI: 10.1590/S1517-83822008000200022     Document Type: Article

References (18)

1. Bendtsen, J.D.; Nielsen, H.; Heijne, G. von; Brunak, S. (2004). Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340, 783-795.
2. Bogo, M.R.; Rota, C.A.; Pinto, J.H.; Ocampos, M.; Correa, C.T.; Vainstein, M.H.; Schrank, A. (1998). A chitinase encoding gene (chit1 gene) from the entomopathogen Metarhizium anisopliae: isolation and characterization of genomic and full-length cDNA. Curr. Microbiol. 37, 221-225.
3. Charnley, A.K.; St. Leger, R.J. (1991). The role of cuticle-degrading enzymes in fungal pathogenesis in insects. In: Cole, E.T., Hoch, H.C. (eds). Fungal spore disease initiation in plants and animals. Plenum Press, NewYork, London, p. 267-287.
4. Chomczynski, P.; Sacchi, N. (1981). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chlorform extraction. Anal. Biochem. 162, 156-159.
5. Fang, W.G.; Leng, B.; Xiao, Y.H.; Y. Pei et al. (2005). Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl. Environ. Microb. 71, 363-370.
6. Gasteiger, E.; Hoogland, C.; Gattiker, A.; Duvaud, S.; Wilkins, M.R.; Appel, R.D.; Bairoch, A. (2005). Protein identification and analysis tools on the ExPASy Server. In: John, M., Walker. (Eds). The proteomics protocols handbook. Humana Press, p. 571-607.
7. Harper, M.; Huang, H.C. (1986). Evaluation of the entomophagous fungus Verticillium lecanii (Moniliales: Moniliaceae) as a control agent for insects. Environ. Entomol. 15, 281-284.
8. Harper, A.M.; Huang, H.C. (1988). Insects killed by the fungus Verticillium lecanii, Canadex No. 621. Agriculture Canada, Ottawa, Ontario.
9. Hollis, T.; Monzingo, A.F.; Bortone, K.; Ernst, S.; Cox, R.; Robertus, J.D. (2000). The X-ray structure of a chitinase from the pathogenic fungus Coccidioides immitis. Protein. Sci. 9, 544-551.
10. Lu, Z.X.; Laroche, A.; Huang, H.C. (2005). Isolation and characterization of chitinases from Verticillium lecanii. Can. J. Microbiol. 51, 1045-1055.
11. Milner, R.J. (1997). Prospects for biopesticides for aphid control. Entomophaga. 42, 227-239.
12. Orikoshi, H.; Baba, N.; Nakayama, S.; Kashu, H.; Miyamoto, K.; Yasuda, M.; Inamori, Y.; Tsujibo, H. (2003). Molecular analysis of the gene encoding a novel cold-adapted chitinase (ChiB) from a marine bacterium, Alteromonas sp. strain O-7. J. Bacteriol. 185, 1153-1160.
13. Reader, U.; Broda, P. (1985). Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1, 17-20.
14. Sambrook, J.; Fritsch, E.F.; Maniatis, T. (1989). Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
15. Schwede, T.; Kopp, J.; Guex, N.; Peitsch, M.C. (2003). SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res. 31, 3381-3385.
16. St. Leger, R.J.; Cooper, R.M.; Charnley, A.K. (1986). Cuticle degrading enzymes of entomopathogenic fungi: regulation of production of chitinolytic enzymes. J. Gen. Microbiol. 132, 1509-1517.
17. Terwisscha van Scheltinga, A.C.; Hennig, M.; Dijkstra, B.W. (1996). The 1.8 Å resolution structure of hevamine a plant chitinase/ lysozyme and analysis of the conserved sequence and structure motifs of glycosyl hydrolase family 18. J. Mol. Biol. 262, 243-257.
18. Wattanalai, R.; Wiwat, C.; Boucias, D.G.; Tartar, A. (2004). Chitinase gene of the dimorphic mycopathogen, Nomuraea rileyi. J. Invertebr. Pathol. 85, 54-57.