Phosphorylation regulates the assembly of chloroplast import machinery

Journal of Experimental Botany

Volumn 59, Issue 9, 2008, Pages 2309-2316

  Oreb, Mislav ^a   Höfle, Anja ^a   Mirus, Oliver ^a   Schleiff, Enrico ^a,b  

^a Department of Biosciences ^*  (Germany)

^b Molecular Plant Sciences   (Germany)

Author keywords

GTPase; Membrane complex dynamics; Phosphorylation; Plastids; Protein complex assembly; Protein translocation; TOC

Indexed keywords

ARABIDOPSIS PROTEIN; GUANOSINE TRIPHOSPHATASE; MEMBRANE PROTEIN; TOC159 PROTEIN, ARABIDOPSIS; TOC34 PROTEIN, ARABIDOPSIS;

ARABIDOPSIS; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; CHLOROPLAST; DIMERIZATION; GENETICS; METABOLISM; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; CHLOROPLASTS; DIMERIZATION; GTP PHOSPHOHYDROLASES; MEMBRANE PROTEINS; MODELS, MOLECULAR; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT;

EID: 48649104944     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/ern095     Document Type: Article

References (34)

1. Allen JF. 2005. Photosynthesis: the processing of redox signals in chloroplasts. Current Biology 15, R929-R932.
2. Aronsson H, Combe J, Patel R, Jarvis P. 2006. In vivo assessment of the significance of phosphorylation of the Arabidopsis chloroplast protein import receptor, atToc33. FEBS Letters 580, 649-655.
3. Becker T, Jelic M, Vojta A, Radunz A, Soll J, Schleiff E. 2004. Preprotein recognition by the Toc complex. EMBO Journal 23, 520-530.
4. Chen D, Schnell DJ. 1997. Insertion of the 34 kDa chloroplast protein import component, IAP34, into the chloroplast outer membrane is dependent on its intrinsic GTP-binding capacity. Journal of Biological Chemistry 272, 6614-6620.
5. Chen K, Chen X, Schnell DJ. 2000. Initial binding of preproteins involving the Toc159 receptor can be bypassed during protein import into chloroplasts. Plant Physiology 122, 813-822.
6. Constan D, Patel R, Keegstra K, Jarvis P. 2004. An outer envelope membrane component of the plastid protein import apparatus plays an essential role in Arabidopsis. The Plant Journal 38, 93-106.
7. Ertel F, Mirus O, Bredemeier R, Moslavac S, Becker T, Schleiff E. 2005. The evolutionarily related beta-barrel polypeptide transporters from Pisum sativum and Nostoc PCC7120 contain two distinct functional domains. Journal of Biological Chemistry 280, 28281-28289.
8. Fiser A, Do RK, Sali A. 2000. Modeling of loops in protein structures. Protein Science 9, 1753-1773.
9. Fulgosi H, Soll J. 2002. The chloroplast protein import receptors Toc34 and Toc159 are phosphorylated by distinct protein kinases. Journal of Biological Chemistry 277, 8934-8940.
10. Gerdes L, Bals T, Klostermann E, Karl M, Philippar K, Hünken M, Soll J, Schünemann D. 2006. A second thylakoid membrane-localized Alb3/OxaI/YidC homologue is involved in proper chloroplast biogenesis in Arabidopsis thaliana. Journal of Biological Chemistry 281, 16632-16642.
11. Glavy JS, Krutchinsky AN, Cristea IM, Berke IC, Boehmer T, Blobel G, Chait BT. 2007. Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex. Proceedings of the National Academy of Sciences, USA 104, 3811-2816.
12. Gutensohn M, Fan E, Frielingsdorf S, Hanner P, Hou B, Hust B, Klösgen RB. 2006. Toc, Tic, Tat et al.: structure and function of protein transport machineries in chloroplasts. Journal of Plant Physiology 163, 333-347.
13. Ivanova Y, Smith MD, Chen K, Schnell DJ. 2004. Members of the Toc159 import receptor family represent distinct pathways for protein targeting to plastids. Molecular Biology of the Cell 15, 3379-3379.
14. Jarvis P, Chen LJ, Li H, Peto CA, Fankhauser C, Chory J. 1998. An Arabidopsis mutant defective in the plastid general protein import apparatus. Science 282, 100-103.
15. Jackson-Constan D, Keegstra K. 2001. Arabidopsis genes encoding components of the chloroplastic protein import apparatus. Plant Physiology 125, 1567-1576.
16. Jelic M, Soll J, Schleiff E. 2003. Two Toc34 homologues with different properties. Biochemistry 42, 5906-5916.
17. Jelic M, Sveshnikova N, Motzkus M, Hörth P, Soll J, Schleiff E. 2002. The chloroplast import receptor Toc34 functions as preprotein regulated GTPase. Biological Chemistry 383, 1875-1883.
18. Jung J, Lee B. 2000. Protein structure alignment using environmental profiles. Protein Engineering 13, 535-543.
19. Kessler F, Schnell DJ. 2006. The function and diversity of plastid protein import pathways: a multilane GTPase highway into plastids. Traffic 7, 248-257.
20. Kikuchi S, Hirohashi T, Nakai M. 2006. Characterization of the preprotein translocon at the outer envelope membrane of chloroplasts by blue native PAGE. Plant and Cell Physiology 47, 363-371.
21. König P, Oreb M, Höfle A, Kaltofen S, Rippe K, Sinning I, Schleiff E, Tews I. 2008. The GTPase cycle of the chloroplast import receptor Toc34: implications from monomeric and dimeric structures. Structure (in press).
22. Krieger E, Darden T, Nabuurs S, Finkelstein A, Vriend G. 2004. Making optimal use of empirical energy functions: force field parameterization in crystal space. Proteins 57, 678-683.
23. Lopez-Juez E. 2007. Plastid biogenesis, between light and shadows. Journal of Experimental Botany 58, 11-26.
24. Oreb M, Reger K, Schleiff E. 2006. Chloroplast protein import: reverse genetic approaches. Current Genome 7, 235-244.
25. Oreb M, Tews I, Schleiff E. 2008. Policing Tic 'n' Toc, the doorway to chloroplasts. Trends in Cell Biology (in press).
26. Oreb M, Zoryan M, Vojta A, Maier UG, Eichacker LA, Schleiff E. 2007. Phospho-mimicry mutant of atToc33 affects early development of Arabidopsis thaliana. FEBS Letters 581, 5945-5951.
27. Sali A, Blundell TL. 1993. Comparative protein modelling by satisfaction of spatial restraints. Journal of Molecular Biology 234, 779-815.
28. Schleiff E, Eichacker LA, Eckart K, Becker T, Mirus O, Stahl T, Soll J. 2003b. Prediction of the plant beta-barrel proteome: a case study of the chloroplast outer envelope. Protein Science 12, 748-759.
29. Schleiff E, Soll J, Küchler M, Kühlbrandt W, Harrer R. 2003a. Characterization of the translocon of the outer envelope of chloroplasts. Journal of Cell Biology 160, 541-551.
30. Smith MD, Hiltbrunner A, Kessler F, Schnell DJ. 2002. The targeting of the atToc159 preprotein receptor to the chloroplast outer membrane is mediated by its GTPase domain and is regulated by GTP. Journal of Cell Biology 159, 833-843.
31. Sun YJ, Forouhar F, Li HM, Tu SL, Yeh YH, Kao S, Shr KL, Chou CC, Chen C, Hsiao CD. 2002. Crystal structure of pea Toc34, a novel GTPase of the chloroplast protein translocon. Nature Structural Biology 9, 95-100.
32. Sveshnikova N, Soll J, Schleiff E. 2000. Toc34 is a preprotein receptor regulated by GTP and phosphorylation. Proceedings of the National Academy of Sciences, USA 97, 4973-4978.
33. Wallas TR, Smith MD, Sanchez-Nieto S, Schnell DJ. 2003. The roles of toc34 and toc75 in targeting the toc159 preprotein receptor to chloroplasts. Journal of Biological Chemistry 278, 44289-44297.
34. Wang X, Johnsson N. 2005. Protein kinase CK2 phosphorylates Sec63p to stimulate the assembly of the endoplasmic reticulum protein translocation apparatus. Journal of Cell Science 118, 723-732.