An Arabidopsis mutant defective in the plastid general protein import apparatus

Science

Volumn 282, Issue 5386, 1998, Pages 100-103

  Jarvis, Paul ^a   Chen, Lih Jen ^b   Li, Hsou Min ^b   Peto, Charles A ^a   Fankhauser, Christian ^a   Chory, Joanne ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b INSTITUTE OF MOLECULAR BIOLOGY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARTICLE; CELL MEMBRANE; CHLOROPLAST; DEVELOPMENT; GENE TRANSLOCATION; NONHUMAN; PHENOTYPE; PLASTID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TRANSPORT; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BIOLOGICAL TRANSPORT; CHLOROPHYLL; CHLOROPLASTS; GENE EXPRESSION REGULATION, PLANT; INTRACELLULAR MEMBRANES; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; SEQUENCE ALIGNMENT;

EID: 0032476027     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5386.100     Document Type: Article

References (32)

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23. The methods used during the in-organelle analysis of plastid protein import were as described [S. E. Perry, H.-m. Li, K. Keegstra, Methods Cell Biol. 34, 327 (1991)].
24. The Toc33 genomic construct comprised a 6.5-kb Xba I restriction fragment of BAC T7I23, which was inserted into the plant transformation vector pPZP221 [P. Hajdukiewicz, Z. Svab, P. Maliga, Plant Mol. Biol. 25, 989 (1994)]. Overexpression constructs comprised Toc33 and Toc34 cDNA clones, which were inserted into a pPZP221-based plant expression vector that carried the cauliflower mosaic virus 355 promoter and a pea ribulose 1,5-bisphosphate carboxylase/oxygenase terminator (pCHf2) [C. Fankhauser, K. Hanson, J. Chory, unpublished data].
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31. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, The; V, Val; W, Trp; and Y, Tyr.
32. We thank J. Lutes for technical assistance; D. Weigel, M. Surpin, and R. Larkin for comments on the manuscript; and G. Armstrong and K. Apel (Eidgenössische Technische Hochschule, Zürich) for providing the POR antiserum. Supported by U.S. Department of Energy grant ER 13993 (J.C.), by Academia Sinica and National Science Council of Taiwan grant NSC 87-2312-B-001-018 (H.-m.L.), and by Human Frontier Science Program fellowships (P.J. and C.F.). J.C. is an associate investigator of the Howard Hughes Medical Institute.