The chloroplast import receptor Toc34 functions as preprotein-regulated GTPase

Biological Chemistry

Volumn 383, Issue 12, 2002, Pages 1875-1883

  Jelic, Marko ^a   Sveshnikova, Natalia ^b   Motzkus, Michael ^b   Hörth, Patric ^c   Soll, Jürgen ^a   Schleiff, Enrico ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF KIEL   (Germany)

^c UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Chloroplast import; GTP binding; Phosphorylation; Preprotein recognition

Indexed keywords

CHLOROPLAST IMPORT RECEPTOR TOC34; ENVELOPE PROTEIN; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; PROTEIN PRECURSOR; PURINE; SERINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; CHLOROPLAST; CONTROLLED STUDY; DOWN REGULATION; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN HYDROLYSIS; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; REGULATORY MECHANISM;

CHLOROPLASTS; CROSS-LINKING REAGENTS; ENZYMES, IMMOBILIZED; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GTP PHOSPHOHYDROLASES; GUANOSINE TRIPHOSPHATE; HYDROLYSIS; MASS SPECTROMETRY; MEMBRANE PROTEINS; MEMBRANES; NUCLEOTIDES; PEPTIDES; PHOSPHOAMINO ACIDS; PHOSPHORYLATION; PHOSPHOSERINE; PLANT PROTEINS; PRECIPITIN TESTS; SEPHAROSE;

EID: 12244310188     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2002.211     Document Type: Article

References (39)

1. Albert, S., Will, E. and Galwitz, D. (1999). Identification of the catalytic domains and their functionally critical arginine residues of two yeast GTPase-activating proteins specific for Ypt/Rab transport GTPases. EMBO J. 18, 5216-5225.
2. Bauer, J., Chen, K., Hiltbunner, A., Wherli, E., Eugster, M., Schnell, D. and Kessler, F. (2000). The major protein import receptor of plastids is essential for chloroplast biogenesis. Nature 403, 203-207.
3. Bölter, B., May, T. and Soll, J. (1998). A protein import receptor in pea chloroplasts, Toc86, is only a proteolytic fragment of a larger polypetide. FEES Lett. 441, 59-62.
4. Bourne, H. R., Sanders, D. A. and McCormick, F. (1991). The GT-Pase superfamily: conserved structure and molecular mechanism. Nature 349, 117-127.
5. Caliebe, A., Grimm, R., Kaiser, G., Lübeck, J., Soll, J. and Heins, L. (1997). The chloroplastic protein import machinery contains a Rieske-type iron-sulfur cluster and a mononuclear iron-binding protein. EMBO J. 16, 7342-7350.
6. Cherfils, J. and Chardin, P. (1999). GEFs: structural basis for their activation of small GTP-binding proteins. Trends Biol. Sci. 24, 306-311.
7. Deltrot, S., Atanassova, R. and Maurousset, L. (2000). Regulation of sugar, amino acid and peptide plant membrane transporters. Biochim. Biophys. Acta 1465, 281-306.
8. Downward, J., Riehl, R., Wu, L. and Weinberg, R. A. (1990). Identification of a nucleotide exchange promoting activity for p21-ras. Proc. Natl. Acad. Sci. USA 87, 5998-6002.
9. Flügge, U. I. and Hinz, G. (1986). Energy dependence of protein translocation into chloroplasts. Eur. J. Biochem. 160, 563-570.
10. Gruss, O., Feick, P., Frank, R. and Dobberstein, B. (1999). Phosphorylation of components of the ER translocation site. Eur. J. Biochem. 260, 785-793.
11. Gutensohn, M., Schulz, B., Nicolay, P. and Flügge, U.-I. (2000). Functional analysis of the two Arabidopsis homologues of Toc34, a component of the chloroplast protein import apparatus. Plant J. 23, 771-783.
12. Hardie, D. G. (1999). Plant protein serine/threonine kinases: classification and functions. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 97-131.
13. Hiltbrunner, A., Bauer, J., Vidi, P. A., Infanger, S., Weibel, R, Hohwy, M. and Kessler, F. (2001). Targeting of an abundant cytosolic form of the protein import receptor atToc159 to the outer chloroplast membrane. J. Cell Biol. 154, 309-316.
14. Hirsch, S., Muckel, E., Heemeyer, F., von Heijne, G. and Soll, J. (1994). A receptor component of the chloroplast protein translocation machinery. Science 266, 1989-1992.
15. Hwang, Y. W. and Miller, D. L. (1987). A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein. J. Biol. Chem. 262, 13081-13085.
16. Keegstra, K. and Cline, K. (1999). Protein import and routing systems of chloroplasts. Plant Cell 11, 557-570.
17. Kessler, F., Blobel, G., Patel, H. A. and Schnell, D. J. (1994). Identification of two GTP-binding proteins in the chloroplast protein import machinery. Science 266, 1035-1039.
18. Kouranov, A. and Schnell, D. J. (1997). Analysis of the interactions of preproteins with the import machinery over the course of protein import into chloroplasts. J. Cell Biol. 139, 1677-1685.
19. May, T. and Soll, J. (1998). Positive charges determine the tropology and functionality of the transmembrane domain in the chloroplastic outer envelope protein Toc34. J. Cell Biol. 141, 895-904.
20. Olsen, L. J. and Keegstra, K. (1992). The binding of precursor proteins to chloroplasts requires nucleoside triphosphates in the intermembrane space. J. Biol. Chem. 267, 433-439.
21. Olsen, L. J., Theg, S. M., Selman, B. R. and Keegstra, K. (1989). ATP is requierd for the binding of precursor proteins to chloroplasts. J. Biol. Chem. 264, 6724-6729.
22. Pain, N. (1996). Initiation of protein synthesis in eukaryotic cells. Eur. J. Biochem 236, 747-771.
23. Robinson, P. J., Sontag, J. M., Liu, J. P., Fykse, E. M., Slaughter, C., McMahon, H. and Sudhof, T. C. (1993). Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminal. Nature 365, 163-166.
24. 2+ is not catalytically required in the intrinsic and kirromycin-stimulatted GTPase action of Thermus thermophilus EF-Tu. J. Biol. Chem. 276, 18728-18733.
25. Scheffzek, K., Ahmadian, M. R. and Wittinghofer, A. (1998). GT-Pase-activating proteins: helping hands to complement an active site. Trends Biol. Sci. 23, 257-262.
26. Schindler, C., Hracky, R. and Soll, J. (1987). Protein transport in chloroplasts: ATP is prerequisite. Z. Naturforsch. 42c, 103-108.
27. Schleiff, E. and Soll, J. (2000). Travelling of proteins through membranes: translocation into chloroplasts. Planta 211, 449-456.
28. Schleiff, E., Soll, J., Sveshnikova, N., Tien, R., Wright, S., Dabney-Smith, C., Subramanian, C. and Bruce, B. D. (2002). Structural and GTP/GDP requirements for transit peptide recognition by the cytosolic domain of the chloroplast outer envelope receptor, Toc34. Biochemistry 41, 1934-1946.
29. Schmidt, G., Lenzen, C., Simon, I., Deuter, R., Cool, R. H., Goody, R. S. and Wittinghofer, A. (1996). Biochemical and biological consequences of changing the specificity of p21ras from guanosine to xanthosine nucleotides. Oncogene 12, 87-96.
30. Schnell, D., Blobel, G., Keegstra, K., Kessler, F., Ko, K. and Schnell, J. (1997). A consensus nomenclature for the protein-import components of the chloroplast envelope. Trends Cell Biol. 7, 303-304.
31. Seedorf, M., Waegemann, K. and Soll, J. (1995). A constituent of the chloroplast import complex represents a new type of GTP-binding protein. Plant J. 7, 401-411.
32. Soll, J., Fischer, I. and Keegstra, K. (1988). A guanosine 5′-triphosphate-dependent protein kinase is localized in the outer envelope of pea chloroplast. Planta 176, 488-496.
33. Su, Q., Schild, C., Schumann, P. and Boschetti, A. (2001). Effect of precursor protein phosphorylation on import into isolated chloroplasts from Chlamydomonas. Eur. J. Biochem. 268, 2315-2321.
34. Sun, Y.-J., Forouhar, F., Li, H.-m., Tu, S.-L., Yeh, Y.-H., Kao, S., Shr, H.-L., Chou, C.-C., Chen, C. and Hsiao, C.-D. (2002). Crystal structure of pea Toc34, a novel GTPase of the chloroplast protein translocon. Nature Struct. Biol. 9, 95-100.
35. Sveshnikova, N., Soll, J. and Schleiff, E. (2000). Toc34 is a preprotein receptor regulated by GTP and phosphorylation. Proc. Natl. Acad. Sci. USA 97, 4973-4978.
36. Waegemann, K. and Soll, J. (1996). Phosphorylation of the transit sequence of chloroplast precursor proteins. J. Biol. Chem. 271, 6545-6554.
37. Wilm, M. and Mann, M. (1996). Analytical properties of the nanoelectrospray ion source. Anal. Chem. 68, 1-8.
38. Young, M. E., Keegstra, K. and Froehlich, J. E. (1999). GTP promotes the formation of early-import intermediates but is not required during the translocation step of protein import into chloroplast. Plant Phys. 121, 237-243.
39. Zhang, B., Wang, Z. X. and Zheng, Y. (1997). Characterisation of the interactions between the small GTPase Cdc42 and its GTPase activating proteins and putative effectors. J. Biol. Chem. 272, 21999-22007.