Application of high-performance tangential flow filtration (HPTFF) to the purification of a human pharmaceutical antibody fragment expressed in Escherichia coli

Biotechnology and Bioengineering

Volumn 100, Issue 5, 2008, Pages 964-974

  Lebreton, Bénédicte ^a   Brown, Arick ^a   Van Reis, Robert ^a  

^a GENENTECH INC   (United States)

Author keywords

High performance tangential flow filtration; Pharmaceutical recombinant proteins; Process development; Protein purification

Indexed keywords

ANTIBODY FRAGMENTS; BUFFER PH; E. COLI; HIGH PERFORMANCE TANGENTIAL FLOW FILTRATION; MOLECULAR WEIGHT CUT OFFS; PHARMACEUTICAL RECOMBINANT PROTEINS; POSITIVELY CHARGED; PROCESS DEVELOPMENT; PROTEIN PURIFICATION; RECOMBINANT PROTEINS; SINGLE UNIT OPERATION; TANGENTIAL FLOW FILTRATION;

CONCENTRATION (PROCESS); ESCHERICHIA COLI; IONIC STRENGTH; POROUS MATERIALS; PROTEINS; PURIFICATION; STEADY FLOW;

FILTRATION;

ANTIBODY; CELL PROTEIN; RECOMBINANT PROTEIN; DRUG; MONOCLONAL ANTIBODY;

ANALYTIC METHOD; ARTICLE; CELL MEMBRANE; CHROMATOGRAPHY; ESCHERICHIA COLI; FILTRATION; HIGH PERFORMANCE TANGENTIAL FLOW FILTRATION; HOST CELL; IONIC STRENGTH; MOLECULAR WEIGHT; NONHUMAN; PH; PROTEIN PURIFICATION; EQUIPMENT; EQUIPMENT DESIGN; FLOW INJECTION ANALYSIS; GENETICS; INSTRUMENTATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROFLUIDICS; ULTRAFILTRATION;

ESCHERICHIA COLI;

ANTIBODIES, MONOCLONAL; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; ESCHERICHIA COLI; FLOW INJECTION ANALYSIS; MICROFLUIDICS; PHARMACEUTICAL PREPARATIONS; ULTRAFILTRATION;

EID: 48449091706     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21842     Document Type: Article

References (25)

1. Aranha-Creado H, Peterson J, Huang PY. 1998. Clearance of murine leukaemia virus from monoclonal antibody solutions by a hydrophilic PVDF microporous membrane filter. Biologicals 26:167-172.
2. Belfort G. 1997. US Patent #5,626,758. Coiled membrane filtration system.
3. Blatt WF, Dravid A, Michaels AS, Nelsen L. 1970. Solute polarization and cake formation in membrane ultrafiltration. Causes, consequences, and control techniques. In: Flinn JE, editor. Membrane science and technology. New York: Plenum Press, pp. 47-97.
4. Brough H, Antoniou C, Carter J, Jakubik J, Xu Y, Lutz H. 2002. Performance of a novel Viresolve" NFR virus filter. Biologicals 18:782-795.
5. Burns DB, Zydney AL. 1999. Effect of solution pH on protein transport through ultrafiltration membranes. Biotech Bioeng 64:27-37.
6. Charlton HR, Relton JM, Slater NKH. 1999. Characterisation of generic monoclonal antibody harvesting system for adsorption of DNA by depth filters and various membranes. Bioseparation 8:281-291.
7. Cheang B, Zydney A. 2003. Separation of a-lactabulmin and b-lactoglobulin using membrane filtration. Biotech Bioeng 83:201-209.
8. Gehlert G, Luque S, Belfort G. 1998. Flux enhancement due to secondary flow in a helical tubular module: 14 ultra and microfiltration of polysaccharides, proteins, and yeast suspensions. Biotechnol Prog 14:931-942.
9. Mehta A, Zydney A. 2006. Effect of membrane charge on flow and protein transport during ultrafiltration. Biotechnol Prog 22:484-492.
10. Millesime L, Dulieu J, Chaufer B. 1996. Fractionation of proteins with modified membranes. Bioseparation 6:135-145.
11. Mulherkar P, van Reis R. 2004. Flex test: A fluorescent dextran test for UF membrane characterization. J Memb Sci 236:171-182.
12. Nakao S, Osada H, Kurada H, Tsuru T, Kimura S. 1988. Separation of proteins by charged ultrafiltration membranes. Desalination 70:191-205.
13. Nystrom M, Aimar P, Luque S, Kulovaara M, Metsamuuronen S. 1998. Fractionation of model proteins using their physicochemical properties. Colloids Surf A Physicochemical Eng Aspects 138:185-205.
14. Pujar NS, Zydney AL. 1998. Electrostatic effects on protein partitioning in size-exclusion chromatography and membrane filtration. J Chromatogr A 796:229-238.
15. Rabiller-Baudry M, Chaufer B, Lucas D, Michel F. 2001. J Memb Sci 184:137-148.
16. Roper DK, Lightfoot LN. 1995. Separation of biomolecules using adsorptive membranes. J Chromatogr A 702:3-26.
17. Saksena S, Zydney AL. 1994. Effect of solution pH and ionic strength on the separation of albumin from immunoglobulins (IgG) by selective filtration. Biotech Bioeng 43:960-968.
18. Santarelli X, Domergue F, Clofent-Sanchez G, Dabadie M, Grissley R, Cassagne C. 1998. Characterization and application of new macroporous membrane ion exchangers. J Chromatogr B 706:13-22.
19. van Reis R. 1993. US Patent #5,256,294. Tangential Flow Filtration Process and Apparatus.
20. van Reis R. 1996. US Patent #5,490,937. Tangential flow filtration process and apparatus.
21. van Reis R, Saksena S. 1997. Optimization diagram for membrane separations. J Membrane Sci 129:19-29.
22. van Reis R, Zydney AL. 1999. Protein ultrafiltration. In: Flickinger MC, Drew SW, editors. Encyclopedia of bioprocess technology: Fermentation, biocatalysis, and bioseparation. New York: John Wiley & Sons, Inc. pp. 2197-2214.
23. wall ultrafiltration process control. J Membrane Sci 130:123-140.
24. van Reis R, Brake JM, Charkoudian J, Burns DB, Zydney AL. 1999. High performance tangential flow filtration using charged membranes. J Membrane Sci 159:133-142.
25. Zeman L, Zydney AL. 1996. Microfiltration and ultrafiltration: Principles and applications. New York: Marcel Dekker.