The crystal structure of [Fe]-hydrogenase reveals the geometry of the active site

Science

Volumn 321, Issue 5888, 2008, Pages 572-575

  Shima, Seigo ^a   Pilak, Oliver ^a   Vogt, Sonja ^a   Schick, Michael ^a   Stagni, Marco S ^b   Meyer Klaucke, Wolfram ^b   Warkentin, Eberhard ^c   Thauer, Rudolf K ^a   Ermler, Ulrich ^c  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON MONOXIDE; CYANIDE; CYSTEINE; HYDROGEN; IRON HYDROGENASE; LIGAND; NITROGEN; PLATINUM; PYRIDINOL; SULFUR; THIOL DERIVATIVE;

CATALYSIS; CRYSTAL STRUCTURE; CYANIDE; ENZYME ACTIVITY; GEOMETRY; LIGAND;

ARTICLE; CHEMICAL BOND; CLOSTRIDIUM PASTEURIANUM; CONTROLLED STUDY; CRYSTAL STRUCTURE; DESULFOVIBRIO DESULFURICANS; DESULFOVIBRIO GIGAS; ENZYME ACTIVE SITE; ENZYME DEGRADATION; ENZYME STRUCTURE; EVOLUTION; GEOMETRY; METHANOCOCCUS JANNASCHII; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL;

APOENZYMES; BINDING SITES; CARBON MONOXIDE; CATALYTIC DOMAIN; COENZYMES; CRYSTALLOGRAPHY, X-RAY; CYANIDES; DIMERIZATION; EVOLUTION, MOLECULAR; HOLOENZYMES; HYDROGEN; HYDROGENASE; IRON; LIGANDS; METHANE; METHANOCOCCALES; MODELS, MOLECULAR; OXIDATION-REDUCTION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY;

EID: 48249129653     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1158978     Document Type: Article

References (33)

1. D. M. Heinekey, W. J. Oldham, Chem. Rev. 93, 913 (1993).
2. G. J. Kubas, Catal. Lett. 104, 79 (2005).
3. C. Tard et al., Nature 433, 610 (2005).
4. T. Liu, M. Y. Darensbourg, J. Am. Chem. Soc. 129, 7008 (2007).
5. A. K. Justice et al., Inorg. Chem. 46, 1655 (2007).
6. S. Ogo et al., Science 316, 585 (2007).
7. X. Hu, B. S. Brunschwig, J. C. Peters, J. Am. Chem. Soc. 129, 8988 (2007).
8. P. M. Vignais, B. Billoud, J. Meyer, FEMS Microbiol. Rev. 25, 455 (2001).
9. A. Volbeda et al., Nature 373, 580 (1995).
10. E. Garcin et al., Structure 7, 557 (1999).
11. H. Ogata et al., J. Am. Chem. Soc. 124, 11628 (2002).
12. A. Volbeda et al., J. Biol. Inorg. Chem. 10, 239 (2005).
13. J. W. Peters, W. N. Lanzilotta, B. J. Lemon, L. C. Seefeldt, Science 282, 1853 (1998).
14. Y. Nicolet, C. Piras, P. Legrand, C. E. Hatchikian, J. C. Fontecilla-Camps, Structure 7, 13 (1999).
15. B. J. Lemon, J. W. Peters, Biochemistry 38, 12969 (1999).
16. Y. Nicolet et al., J. Am. Chem. Soc. 123, 1596 (2001).
17. Z. Chen et al., Biochemistry 41, 2036 (2002).
18. M. Frey, ChemBioChem 3, 153 (2002).
19. A. Silakov, E. J. Reijerse, S. P. J. Albracht, E. C. Hatchikian, W. Lubitz, J. Am. Chem. Soc. 129, 11447 (2007).
20. S. Shima, R. K. Thauer, Chem. Rec. 7, 37 (2007).
21. G. Buurman, S. Shima, R. K. Thauer, FEBS Lett. 485, 200 (2000).
22. E. J. Lyon et al., Eur. J. Biochem. 271, 195 (2004).
23. S. Shima et al., Angew. Chem. Int. Ed. 43, 2547 (2004).
24. E. J. Lyon et al., J. Am. Chem. Soc. 126, 14239 (2004).
25. M. Korbas et al., J. Biol. Chem. 281, 30804 (2006).
26. S. Shima, E. J. Lyon, R. K. Thauer, B. Mienert, B. Bill, J. Am. Chem. Soc. 127, 10430 (2005).
27. O. Pilak et al., J. Mol. Biol. 358, 798 (2006).
28. See supporting material on Science Online.
29. J. M. Rawson, R. E. P. Winpenny, Coord. Chem. Rev. 139, 313 (1995).
30. S. Wolfe, N. Weinberg, Y. Hsieh, Theor. Chem. Acc. 118, 265 (2007).
31. A. Böck, P. W. King, M. Blokesch, M. C. Posewitz, Adv. Microb. Physiol. 51, 1 (2006).
32. S. Vogt, E. J. Lyon, S. Shima, R. K. Thauer, J. Biol. Inorg. Chem. 13, 97 (2008).
33. 2 project). We thank H. Michel for continuous support, and the staff of beamline PXII at the Swiss Light Source for help during data collection. Coordinates of [Fe]-hydrogenase and [Fe]-hydrogenase complexed with cyanide have been deposited in the Research Collaboratory for Structural Bioinformatics Protein Data Bank under accession codes 3DAG and 3DAF.