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It should be noted that the most detailed ENDOR and Mössbauer experiments have been performed on hydrogenase II from C. pasteurianum of which no crystal structure is available. Up to now, however, no follow-up studies of any kind have been published on hydrogenase II. Also, we were not able to find a gene encoding for this hydrogenase in the genome databases e.g, UNI-PROT, Therefore, it cannot be completely excluded that hydrogenase II is an artifact, e.g. a partly denatured hydrogenase I enzyme. Since in the meantime hydrogenase I of C. pasteurianum has been characterized very well, it may be worthwhile to repeat the ENDOR and Mössbauer experiments on this enzyme to verify the reported 57Fe hyperfine values
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