Isolation of monocytes from leukapheretic products for large-scale GMP-grade generation of cytomegalovirus-specific T-cell lines by means of an automated elutriation device

Transfusion

Volumn 48, Issue 8, 2008, Pages 1644-1649

  Perseghin, Paolo ^a   D'Amico, Giovanna ^b   Dander, Erica ^b   Gaipa, Giuseppe ^b   Dassi, Maria ^b   Biagi, Ettore ^b   Biondi, Andrea ^b  

^a SAN GERARDO HOSPITAL   (Italy)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA INTERFERON; GAMMA INTERFERON; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERLEUKIN 1BETA; INTERLEUKIN 4; POLYINOSINIC POLYCYTIDYLIC ACID; TUMOR NECROSIS FACTOR ALPHA; VIRUS PROTEIN;

ARTICLE; AUTOMATION; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL DIFFERENTIATION; CELL FRACTIONATION; CELL ISOLATION; CELL LINE; CELL SEPARATION; CELL STIMULATION; CYTOMEGALOVIRUS; DENDRITIC CELL; DEVICE; HUMAN; HUMAN CELL; LARGE SCALE PRODUCTION; LEUKAPHERESIS; MONOCYTE; SEPARATION TECHNIQUE;

ANTIGEN PRESENTATION; ANTIGENS, VIRAL; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CELL LINE; CYTOMEGALOVIRUS INFECTIONS; DENDRITIC CELLS; EPITOPES; HUMANS; IMMUNOMAGNETIC SEPARATION; LEUKAPHERESIS; MONOCYTES;

EID: 48249125852     PISSN: 00411132     EISSN: 15372995     Source Type: Journal    
DOI: 10.1111/j.1537-2995.2008.01756.x     Document Type: Article

References (16)

1. Fong L, Hou Y, Rivas A, Benike C, Yuen A, Fisher GA, Davis MM, Engleman EG. Altered peptide ligand vaccination with fltr3 ligand expanded dendritic cells for tumor immunotherapy. Proc Natl Acad Sci U S A 2001 98/ 15 : 8809 14.
2. Berger TG, Strasser E, Smith R, Carste C, Schuler-Thurner B, Kaempgen E, Schuler G. Efficient elutriation of monocytes within a closed system (Elutra™) for clinical-scale generation of dendritic cells. J Immunol Methods 2005 298 : 61 72.
3. Trived D, Williams RY, O'Reilly RJ, Koehne G. Generation of CMV-specific T lymphocytes using protein-spanning pools of pp65-derived overlapping pentadecapeptides for adoptive immunotherapy. Blood 2005 105 : 2793 801.
4. Schwanke U, Nabereit A, Moog R. Isolation of monocytes from whole blood-derived buffy coats by continuous counter-flow elutriation. J Clin Apheresis 2006 21 : 153 7.
5. Perseghin P, Dassi M, Balduzzi A, Rovelli A, Bonanomi S, Uderzo C. Mononuclear cell collection in patients undergoing extra-corporeal photo-chemotherapy for acute and chronic graft-vs.-host-disease (GvHD): comparison between COBE Spectra version 4.7 and 6.0 (AutoPBSC). J Clin Apheresis 2002 17 : 65 71.
6. Dander E, Li Pira G, Biagi E, Perseghin P, Renoldi G, Gaipa G, Introna M, Marin V, Manca F, Biondi A, D'Amico G. Characterization of migratory activity and cytokine profile of helper and cytotoxic CMV-specific T-cell lines expanded by a selective peptide library. Exp Hematol 2008 36 : 473 85.
7. Thurner B, Roder C, Dieckeman D, Heuer M, Kruse M, Glaser A, Keikavoussi P, Kampgen E, Bender A, Schuler G. Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical application. J Immunol Methods 1999 223 : 1 15.
8. Suen Y, Lee SM, Aono F, Hou S, Loudovaris M, Ofstein G, Bender JG. Comparison of monocyte enrichement by immunomagnetic depletion or adherence for the clinical-scale generation of DC. Cytotherapy 2001 3 : 365 75.
9. Padley DJ, Dietz AB, Gastineau DA, Vuk-Pavlovic S. Mature myeloid dendritic cells for clinical use prepared from CD14+ cells isoalted by immunomagnetic adsorption. J Hematother Stem Cell Res 2001 10 : 427 9.
10. Rouard H, Leon A, De Reys S, Taylor L, Logan J, Marquet J, Jouault H, Loper K, Maison P, Delfau-Larue MH, Beaujean F, Farcet JP, Noga SJ. A closed and single-use system for monocyte enrichment: potential for dendritic cell generation for clinical application. Transfusion 2003 43 : 481 7.
11. Ramadan G, Konings S, Kurup VP, Keever-Taylor CA. Generation of Aspergillus and CMV- specific T-cell responses using autologous fast DC. Cytotherapy 2004 6 : 223 34.
12. Grübe M, Melenhorst JJ, Barrett AJ. An APC for every occasion: induction and expansion of human Ag-specific CD4 and CD8 T cells using cellular and non-cellular APC. Cytotherapy 2004 6 : 440 9.
13. D'Amico G, Bonamino M, Dander E, Marin V, Basso G, Balduzzi A, Biagi E, Biondi A. T cells stimulated by CD40L positive leukemic blasts-pulsed dendritic cells meet optimal functional requirements for adoptive T-cell therapy. Leukemia 2006 20 : 2015 24.
14. ten Brinke A, Karsten ML, Dieker MC, Zwaginga JJ, Vrielink H, van Hams MS. Generation of dendritic cells for immunotherapy is minimally impaired by granulocytes in the monocytes preparation. Immunobiology 2006 211 : 633 40.
15. Erdmann M, Dörrie J, Schaft N, Strasser E, Hendelmeier M, Kämpgen E, Schuler G, Schuler-Thurner B. Effective clinical-scale production of dendritic cell vaccines by monocyte elutriation directly in medium, subsequent culture in bags and final antigen loading using peptides or RNA transfection. J Immunther 2007 30/ 6 : 663 74.
16. Sinyoung K, Hyun Ok K, Eun-Jung B, Youjeong C, Han-Soo K, Min-Geul L. Monocyte enrichment from leukapheresis products by using the Elutra cell separator. Transfusion 2007 47 : 2290 6.