Characterisation and identification of arbuscular mycorrhizal fungi species by PCR/RFLP analysis of the rDNA internal transcribed spacer (ITS)

Annals of Microbiology

Volumn 58, Issue 2, 2008, Pages 341-344

  Do Espírito Santo Mergulhão, Adália Cavalcanti ^a   Da Silva, Márcia Vanusa ^b   Do Vale Barreto Figueiredo, Márcia ^a   Burity, Hélio Almeida ^a   Maia, Leonor Costa ^b  

^a Laboratorio de Biologia do Solo   (Brazil)

^b FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

Author keywords

AM fungi; Glomus and paraglomus species; ITS

Indexed keywords

ARBUSCULAR; FUNGI; GLOMUS; GLOMUS ETUNICATUM; GLOMUS INTRARADICES; GLOMUS MOSSEAE; PARAGLOMUS; PARAGLOMUS OCCULTUM;

EID: 47949127643     PISSN: 15904261     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf03175340     Document Type: Article

References (21)

1. Antoniolli Z.I., Schachtman D.P., Ophel-Keller K., Smith S.E. (2000). Variation in rDNA ITS sequences in Glomus mosseae and Gigaspora margarita spores from a permanent pasture. Mycol. Res., 104: 708-715.
2. Buscot F., Munch J.C., Charcosset J.Y., Gardes M., Nehls U., Hampp R. (2000). Recent advances in exploring physiology and biodiversity of ectomycorrhizas highlight the functioning of these symbioses in ecosystems. FEMS Microbiol. Rev., 24: 601-614.
3. Dickie L.A., FitzJohn R.G. (2007). Using terminal restriction fragment length polymorphism (T-RFLP) to identify mycorrhizal fungi: a methods review. Mycorrhiza, 17: 259-270.
4. Camper H. Leuchtmann A. (2007). Taxon-specific PCR primers to detect two inconspicuous arbuscular mycorrhizal fungi from temperate agricultural grassland. Mycorrhiza, 17: 145-152.
5. Gerdemann J.W., Nicolson T.H. (1963). Spore of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc., 46: 235-244.
6. Grifoni A., Bazzicalupo M., Di Seri C., Fancelli S., Fani R. (1995). Identification of Azospirillum strais by restriction fragment lenght polymorphism of 16S rDINA and of the histidine operon. FEMS Microbiol. Lett., 127: 85-91.
7. Harney S.K., Edwards F.S., Allen M.F. (1997). Identification of arbuscular mycorrhizal fungi from Artemisia californica using the polimerase chain reaction. Mycologia, 89: 547-550.
8. Hempel S., Renker C., Buscot F. (2007). Differences in the species composition of arbuscular mycorrhizal fungi in spore, root and soil communities in a grassland ecosystem. Environ. Microbiol., 9: 1930-1938.
9. Hildebrandt U., Janetta K., Ouziad F., Renne B., Nawrath K., Bothe H. (2001). Arbuscular mycorrhizal colonization of halophytes in Central European salt marshes. Mycorrhiza, 10: 175-183.
10. Horton T.R., Bruns T.D. (2001). The molecular revolution in ectomycorrhizal ecology: peeking into the black-box. Mol. Ecol., 10: 1855-1871.
11. Jenkins W.R. (1964). A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Dis. Rep., 48: 692.
12. Lanfranco, L., Delpero, M., Bonfante P. (1999). Intrasporal variability of ribosomal sequences in the endomycorrhizal fungus Gigaspora margarita. Mol. Ecol., 8: 37-45.
13. Lanfranco L., Bianciotto V., Lumini E., Souza M., Morton J.B., Bonfante P.A. (2001). Combined morphological and molecular approach to characterize isolates of arbuscular mycorrhizal fungi in Gigaspora (Glomales). New Phytol., 152: 169-179.
14. Redecker D., Thierfelder H., Walker C., Werner D. (1997). Restriction analysis of PCR-amplified internal transcribed spacers of ribosomal DNA as a tool for species identification in different genera of the order Glomales. Appl. Environ. Microbiol., 63: 1756-1761.
15. Redecker D., Morton J.B., Bruns T.D. (2000). Ancestral lineages of arbuscular mycorrhizal fungi (Glomales). Mol. Phylogenet. Evol., 14: 276-284.
16. Renker, C., Heinrichs, J., Kaidorf, M., Buscot, F. (2003). Combining nested PCR and restriction digest of the internal transcribed spacer region to characterize arbuscular mycorrhizal fungi on roots from the field. Mycorrhiza, 13: 191-198.
17. Renker, C., Weißhuhn, K., Kellner, H., Buscot, F. (2006). Rationalizing molecular analysis of field-collected roots for assessing diversity of arbuscular mycorrhizal fungi: to pool, or not to pool, that is the question. Mycorrhiza, 16: 525-531.
18. Sanders J.R., Alt M., Groope K., Boiler T., Wiemken A. (1995). Identification of ribosomal DNA polymorphisms among and within spores of the Glomales: application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytol., 130: 419-427.
19. rd edn., Synergistic Publ., Gainesville.
20. Smith S.E., Read D.J. (1997). Mycorrhizal Symbiosis, 2nd edn., Academic Press, London, UK.
21. White T.J., Bruns T., Lee S., Taylor J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., Eds, PCR Protocols: A Guide to Methods and Applications, Academic, San Diego, pp. 315-322.