Cytotoxic and phenotypic effects of uranium and lead on osteoblastic cells are highly dependent on metal speciation

Toxicology

Volumn 250, Issue 1, 2008, Pages 62-69

  Milgram, S ^a   Carriere M ^a   Thiebault, C ^a   Malaval, L ^b   Gouget, B ^a  

^a CNRS   (France)

^b UNIVERSITÉ JEAN MONNET   (France)

Author keywords

Cytotoxicity; Lead; Osteoblasts; Phenotypic effects; Speciation; Uranium

Indexed keywords

LEAD; OSTEOCALCIN; SIALOPROTEIN; URANIUM;

ANIMAL CELL; ARTICLE; BONE MINERALIZATION; CONTROLLED STUDY; CYTOTOXICITY; INTOXICATION; NONHUMAN; OSSIFICATION; OSTEOBLAST; PHENOTYPE; PRIORITY JOURNAL;

ANIMALS; CALCIFICATION, PHYSIOLOGIC; CELL LINE; CELL LINE, TUMOR; HUMANS; LEAD; OSTEOBLASTS; OSTEOCALCIN; OSTEOGENESIS; PHENOTYPE; RATS; SIALOGLYCOPROTEINS; URANIUM COMPOUNDS;

RATTUS;

EID: 47849087932     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tox.2008.06.003     Document Type: Article

References (40)

1. Aleo M.F., Bettoni F., Boniotti J., Morandini F., Giuliani R., Steimberg N., Apostoli P., and Mazzoleni G. A comparative in vitro study of the toxic potency of five inorganic lead compounds on a rat liver epithelial cell line (REL). Toxicol. In vitro 20 (2006) 874-881
2. Ansoborlo E., Prat O., Moisy P., Den Auwer C., Guilbaud P., Carriere M., Gouget B., Duffield J., Doizi D., Vercouter T., Moulin C., and Moulin V. Actinide speciation in relation to biological processes. Biochimie 88 (2006) 1605-1618
3. Bagchi D., and Preuss H.G. Effects of acute and chronic oval exposure of lead on blood pressure and bone mineral density in rats. J. Inorg. Biochem. 99 (2005) 1155-1164
4. Barry P.S. A comparison of concentrations of lead in human tissues. Br. J. Ind. Med. 32 (1975) 119-139
5. Berridge M.V., and Tan A.S. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch. Biochem. Biophys. 303 (1993) 474-482
6. Bianco P., Riminucci M., Bonucci E., Termine J., and Robey P. Bone sialoprotein (BSP) secretion and osteoblast differentiation: relationship to bromodeoxyuridine incorporation, alkaline phosphatase, and matrix deposition. J. Histochem. Cytochem. 41 (1993) 183-191
7. Bion L. BASSIST: an applied thermodynamic database for radionuclides chemistry. Radiochim. Acta 91 (2003) 633-638
8. Carmouche J.J., Puzas J.E., Zhang X., Tiyapatanaputi P., Cory-Slechta D.A., Gelein R., Zuscik M., Rosier R.N., Boyce B.F., O'Keefe R.J., and Schwarz E.M. Lead exposure inhibits fracture healing and is associated with increased chondrogenesis, delay in cartilage mineralization, and a decrease in osteoprogenitor frequency. Environ. Health Perspect. 113 (2005) 749-755
9. Carriere M., Avoscan L., Collins R., Carrot F., Khodja H., Ansoborlo E., and Gouget B. Influence of uranium speciation on normal rat kidney (NRK-52E) proximal cell cytotoxicity. Chem. Res. Toxicol. 17 (2004) 446-452
10. Carriere M., Thiebault C., Milgram S., Avoscan L., Proux O., and Gouget B. Citrate does not change uranium chemical speciation in cell culture medium but increases its toxicity and accumulation in NRK-52E cells. Chem. Res. Toxicol. 19 (2006) 1637-1642
11. Chipperfield A.R., and Taylor D.M. The binding of americium and plutonium to bone glycoproteins. Eur. J. Biochem. 17 (1970) 581-585
12. Cohen Jr. M.M. The new bone biology: pathologic, molecular, and clinical correlates. Am. J. Med. Genet. A 140 (2006) 2646-2706
13. Craft E.S., Abu-Qare A.W., Flaherty M.M., Garofolo M.C., Rincavage H.L., and Abou-Donia M.B. Depleted and natural uranium: chemistry and toxicological effects. J. Toxicol. Environ. Health B Crit. Rev. 7 (2004) 297-317
14. Dowd T.L., Rosen J.F., and Gupta R.K. 31P NMR and saturation transfer studies of the effect of Pb2+ on cultured osteoblastic bone cells. J. Biol. Chem. 265 (1990) 20833-20838
15. Dowd T.L., Rosen J.F., Mints L., and Gundberg C.M. The effect of Pb2+ on the structure and hydroxyapatite binding properties of osteocalcin. Biochim. Biophys. Acta 1535 (2001) 153-163
16. Duffus J.H. Heavy metals. A meaningless term?. Pure Appl. Chem. 74 (2002) 793-807
17. Fukuda S., Ikeda M., Chiba M., and Kaneko K. Clinical diagnostic indicators of renal and bone damage in rats intramuscularly injected with depleted uranium. Radiat. Prot. Dosimetry 118 (2006) 307-314
18. Goering P.L. Lead-protein interactions as a basis for lead toxicity. Neurotoxicology 14 (1993) 45-60
19. Klein R.F., and Wiren K.M. Regulation of osteoblastic gene expression by lead. Endocrinology 132 (1993) 2531-2537
20. Landrigan P.J., and Todd A.C. Lead poisoning. West. J. Med. 161 (1994) 153-159
21. Leggett R.W. The behavior and chemical toxicity of U in the kidney: a reassessment. Health Phys. 57 (1989) 365-383
22. Leggett R.W. Basis for the ICRP's age-specific biokinetic model for uranium. Health Phys. 67 (1994) 589-610
23. Long G.J., Rosen J.F., and Pounds J.G. Lead impairs the production of osteocalcin by rat osteosarcoma (ROS 17/2.8) cells. Toxicol. Appl. Pharmacol. 106 (1990) 270-277
24. Miller A.C., Blakely W.F., Livengood D., Whittaker T., Xu J., Ejnik J.W., Hamilton M.M., Parlette E., John T.S., Gerstenberg H.M., and Hsu H. Transformation of human osteoblast cells to the tumorigenic phenotype by depleted uranium-uranyl chloride. Environ. Health Perspect. 106 (1998) 465-471
25. Miller A.C., Brooks K., Stewart M., Anderson B., Shi L., McClain D., and Page N. Genomic instability in human osteoblast cells after exposure to depleted uranium: delayed lethality and micronuclei formation. J. Environ. Radioact. 64 (2003) 247-259
26. Miller A.C., Stewart M., Brooks K., Shi L., and Page N. Depleted uranium-catalyzed oxidative DNA damage: absence of significant alpha particle decay. J. Inorg. Biochem. 91 (2002) 246-252
27. 1): influence of uranium speciation. Toxicol. Lett. 104 (1999) 249-256
28. Mistry P., Lucier G.W., and Fowler B.A. High-affinity lead binding proteins in rat kidney cytosol mediate cell-free nuclear translocation of lead. J. Pharmacol. Exp. Ther. 232 (1985) 462-469
29. Muller D., Houpert P., Cambar J., and Henge-Napoli M.-H. Role of the sodium-dependent phosphate co-transporters and of the phosphate complexes of uranyl in the cytotoxicity of uranium in LLC-PK1 cells. Toxicol. Appl. Pharmacol. 214 (2006) 166-177
30. Ong C.N., and Lee W.R. Distribution of lead-203 in human peripheral blood in vitro. Br. J. Ind. Med. 37 (1980) 78-84
31. Potula V., Kleinbaum D., and Kaye W. Lead exposure and spine bone mineral density. J. Occup. Environ. Med. 48 (2006) 556-564
32. Pounds J.G., Long G.J., and Rosen J.F. Cellular and molecular toxicity of lead in bone. Environ. Health Perspect. 91 (1991) 17-32
33. Sauk J.J., Smith T., Silbergeld E.K., Fowler B.A., and Somerman M.J. Lead inhibits secretion of osteonectin/SPARC without significantly altering collagen or Hsp47 production in osteoblast-like ROS 17/2.8 cells. Toxicol. Appl. Pharmacol. 116 (1992) 240-247
34. Schanne F.A., Dowd T.L., Gupta R.K., and Rosen J.F. Lead increases free Ca2+ concentration in cultured osteoblastic bone cells: simultaneous detection of intracellular free Pb2+ by 19F NMR. Proc. Natl. Acad. Sci. U.S.A. 86 (1989) 5133-5135
35. Schwartz J., Angle C., and Pitcher H. Relationship between childhood blood lead levels and stature. Pediatrics 77 (1986) 281-288
36. Tasat D.R., Orona N.S., Mandalunis P.M., Cabrini R.L., and Ubios A.M. Ultrastructural and metabolic changes in osteoblasts exposed to uranyl nitrate. Arch. Toxicol. 81 (2007) 319-326
37. Thiebault C., Carriere M., Milgram S., Simon A., Avoscan L., and Gouget B. Uranium induces apoptosis and is genotoxic to normal rat kidney (NRK-52E) proximal cells. Toxicol. Sci. 98 (2007) 479-487
38. Ubios A.M., Guglielmotti M.B., Steimetz T., and Cabrini R.L. Uranium inhibits bone formation in physiologic alveolar bone modeling and remodeling. Environ. Res. 54 (1991) 17-23
39. Van der Lee, J., De Windt, L., 2002. CHESS Tutorial and cookbook/version 3.0. Users Manuel LHM/RD/02/13, Ecole des Mines de Paris, Fontainebleau.
40. 2(2+) to apotransferrin: can this protein account for entry of uranium into human cells?. Biochemistry 46 (2007) 2215-2226