Comparison of PaprE, PamyE, and PP43 promoter strength for β-galactosidae and staphylokinase expression in Bacillus subtilis

Biotechnology and Bioprocess Engineering

Volumn 13, Issue 3, 2008, Pages 313-318

  Kim, June Hyung ^a   Hwang, Bum Yeol ^b   Roh, Jiwon ^a   Lee, Jong Ki ^a   Kim, Kwang ^a   Wong, Sui Lam ^c   Yun, Hyungdon ^d   Lee, Sun Gu ^e   Kim, Byung Gee ^b  

^a Dong A University   (South Korea)

^b Seoul National University   (South Korea)

^c UNIVERSITY OF CALGARY   (Canada)

^d Yeungnam University   (South Korea)

^e Pusan National University   (South Korea)

Author keywords

galactosidase; Bacillus subtilis; Expression system; Promoter; Staphylokinase

Indexed keywords

ALKALINE PROTEINASE; AMYLASE; BETA GALACTOSIDASE; CYTIDINE; STAPHYLOKINASE;

ARTICLE; BACILLUS SUBTILIS; CHROMOSOME; CONTROLLED STUDY; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENETIC TRANSCRIPTION; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 47849086308     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-007-0102-0     Document Type: Article

References (29)

1. Kim, S. H., B. H. Shin, Y. H. Kim, S. W. Nam, and S. J. Jeon (2007) Cloning and expression of a full-length glutamate decarboxylase gene from Lactobacillus brevis BH2. Biotechnol. Bioprocess Eng. 12: 707-712.
2. Shi, F., Z. Xu, and P. Cen (2006) Optimization of γ-polyglutamic acid production by Bacillus subtilis ZJU-7 using a surface-response methodology. Biotechnol. Bioprocess Eng. 11: 251-257.
3. Jung, Y. J., J. S. Yoo, Y. S. Lee, I. H. Park, S. H. Kim, S. C. Lee, M. Yasuda, S. Y. Chung, and Y. L. Choi (2007) Purification and characterization of thermostable β-1,3-1,4 glucanase from Bacillus sp. A8-8. Biotechnol. Bioprocess Eng. 12: 265-270.
4. Kerovuo, J., N. von Weymarn, M. Povelainen, S. Auer, and A. Miasnikov (2000) A new efficient expression system for Bacillus and its application to production of recombinant phytase. Biotechnol. Lett. 22: 1311-1317.
5. Bron, S., P. Bosma, M. Van Belkum, and E. Luxen (1987) Stability function in the Bacillus subtilis plasmid pTA1060. Plasmid 18: 8-15.
6. Nagarajan, V., R. Ramaley, H. Albertson, and M. Cheu (1993) Secretion of streptividine from Bacillus subtilis. Appl. Environ. Microbiol. 59: 3894-3898.
7. Kontinen, V. P. and M. Sarvas (1993) The PrsA lipoprotein is essential for protein secretion in Bacillus subtilis and sets a limit for high-level secretion. Mol. Microbiol. 8: 727-737.
8. Wu, X. C., W. Lee, L. Tran, and S. L. Wong (1991) Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases. J. Bacteriol. 173: 4952-4958.
9. Lee, J. and S. J. Parulekar (1993) Enhanced production of α-amylase in fed-batch cultures of Bacillus subtilis TN106[pAT5]. Biotechnol. Bioeng. 42: 1142-1150.
10. Letouvet-Pawlak, B., C. Monnier, S. Barray, D. A. Hodgson, and J. F. Guespin-Michel (1990) Comparison of beta-galactosidase production by two inducible promoters in Myxococcus xanthus. Res. Microbiol. 141: 425-435.
11. Mumberg, D., R. Muller, and M. Funk (1995) Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122.
12. Nacken, V., T. Achstetter, and E. Degryse (1996) Probing the limits of expression levels by varying promoter strength and plasmid copy number in Saccharomyces cerevisiae. Gene 175: 253-260.
13. Ye, R., J. H. Kim, B. G. Kim, S. Szarka, E. Sihota, and S. L. Wong (1999) High-level secretory production of intact, biologically active staphylokinase from Bacillus subtilis. Biotechnol. Bioeng. 62: 87-96.
14. Broadwell, A. H., L. Baumann, and P. Baumann (1990) The 42- and 51-kilodalton mosquitocidal proteins of Bacillus sphaericus 2362: construction of recombinants with enhanced expression and in vivo studies of processing and toxicity. J. Bacteriol. 172: 2217-2223.
15. Nicholson, W. L., Y. K. Park, T. M. Henkin, M. Won, M. J. Weickert, J. A. Gaskell, and G. H. Chambliss (1987) Catabolite repression-resistant mutations of the Bacillus subtilis alpha-amylase promoter affect transcription levels and are in an operator-like sequence. J. Mol. Biol. 198: 609-618.
16. Wang, P. Z. and R. H. Doi (1984) Overlapping promoters transcribed by Bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases. J. Biol. Chem. 259: 8619-8625.
17. Wang, J. J., K. Rojanatavorn, and J. C. H. Shih (2004) Increased production of Bacillus keratinase by chromosomal integration of multiple copies of the kerA gene. Biotechnol. Bioeng. 87: 459-464.
18. Li, Y., F. Lu, T. Sun, and L. Du (2007) Expression of ksdD gene encoding 3-ketosteroid-delta1-dehydrogenase from Arthrobacter simplex in Bacillus subtilis. Lett. Appl. Microbiol. 44: 563-568.
19. Nicholson, W. L. and P. Setlow (1990) Sporulation, germination and outgrowth. pp. 391-450. In: C. Harwood and S. Cutting (eds.). Molecular Biological Methods for Bacillus. John Wiley & Sons, Ltd., Chichester, UK.
20. Ferrari, E., D. J. Henner, M. Perego, and J. A. Hoch (1988) Transcription of Bacillus subtilis subtilisin and expression of subtilisin in sporulation mutants. J. Bacteriol. 170: 289-295.
21. Song, B. H. and J. Neuhard (1989) Chromosomal location, cloning and nucleotide sequence of the Bacillus subtilis cdd gene encoding cytidine/deoxycytidine deaminase. Mol. Gen. Genet. 216: 462-468.
22. Collen, D. and H. R. Lijnen (1994) Staphylokinase, a fibrin-specific plasminogen activator with therapeutic potential? Blood 84: 680-686.
23. Collen, D. and F. van de Werf (1993) Coronary thrombolysis with recombinant staphylokinase in patients with evolving myocardial infarction. Circulation 87: 1850-1853.
24. Tanaka, T., M. Kawata, Y. Nagami, and H. Uchiyama (1987) prtR enhances the mRNA level of the Bacillus subtilis extracellular proteases. J. Bacteriol. 169: 3044-3050.
25. Henner, D. J., E. Ferrari, M. Perego, and J. A. Hoch (1988) Location of the targets of the hpr-97, sacU32(Hy), and sacQ36(Hy) mutations in upstream regions of the subtilisin promoter. J. Bacteriol. 170: 296-300.
26. McCready, P. M. and R. H. Doi (1992) Bacillus subtilis SenS exerts its activity through a site in the 5′ flanking region of the aprE promoter. J. Gen. Microbiol. 138: 2069-2074.
27. Ogura, M., M. Kawata-Mukai, M. Itaya, K. Takio, and T. Tanaka (1994) Multiple copies of the proB gene enhance degS-dependent extracellular protease production in Bacillus subtilis. J. Bacteriol. 176: 5673-5680.
28. Henkin, T. M., F. J. Grundy, W. L. Nicholson, and G. H. Chambliss (1991) Catabolite repression of alpha-amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacl and galR repressors. Mol. Microbiol. 5: 575-584.
29. Kim, J. H., S. L. Wong, and B. G. Kim (2001) Optimization of staphylokinase production in Bacillus subtilis using inducible and constitutive promoters. Biotechnol. Bioprocess Eng. 6: 167-172.