Expression of ksdD gene encoding 3-ketosteroid-Δ1- dehydrogenase from Arthrobacter simplex in Bacillus subtilis

Letters in Applied Microbiology

Volumn 44, Issue 5, 2007, Pages 563-568

  Li, Y ^a   Lu, F ^a   Sun, T ^a   Du, L ^a  

^a TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

3 ketosteroid 1 dehydrogenase; Arthrobacter simplex; Bacillus subtilis; Expression; Steroid transformation

Indexed keywords

BACTERIOLOGY; GENE ENCODING; GENE EXPRESSION;

3-KETOSTEROID-Δ1-DEHYDROGENASE; ARTHROBACTER SIMPLEX; B.SUBTILIS; BACILLUS SUBTILIS; ENZYMES ACTIVITY; EXPRESSION; GENES ENCODING; RECOMBINANT CELLS; SDS-PAGE ANALYSIS; STEROID TRANSFORMATION;

ENZYME ACTIVITY;

3 KETOSTEROID DELTA1 DEHYDROGENASE; ANDROST 1 ENE 3,17 DIONE; STEROID REDUCTASE; UNCLASSIFIED DRUG;

ENZYME ACTIVITY; GENE ENCODING; MOLECULAR WEIGHT; PROTEINS;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; STEROID;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; BACTERIAL STRAIN; ENZYME ACTIVITY; GENE; GENE EXPRESSION; KSDD GENE; MOLECULAR WEIGHT; NONHUMAN; PIMELOBACTER SIMPLEX; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SPECTROPHOTOMETRY; STATISTICAL SIGNIFICANCE;

BACILLUS SUBTILIS; PIMELOBACTER SIMPLEX;

EID: 34247325673     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02134.x     Document Type: Article

References (20)

1. Choi, K.P., Molnar, I.J., Yamashita, M. Murooka, Y. (1995a) Purification and characterization of the 3-ketosteroid-delta 1-dehydrogenase of Arthrobacter simplex produced in Streptomyces lividans. Biochem (Tokyo) 5, 1043 1049.
2. Choi, K.P., Molnár, I. Murooka, Y. (1995b) Secretory overproduction of Arthrobacter simplex 3-ketosteroid Δl-dehydrogenase by Streptomyces lividans with a multi-copy shuttle vector. Appl Microbiol Biotechnol 43, 1044 1049.
3. Cirilo, G.M., Jorge, R.L. Pedro, J.N. (1993) Synthesis and 1H and 13C Nuclear Magnetic Resonance of 16-Methylene-17α-Hydroxypregna-1,4,9(11)- Triene-3,20-Dione. Steroids 58, 396 399.
4. David, E.D., Gunnar, V.H., Nordlund, P. Jan-Willem, L.G. (2001) Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Letters 507, 220 224.
5. Doi, R.H., Wong, S.L. Kawamura, F. (1998) Potential use of Bacillus subtilis for secretion and production of foreign proteins. Trends Biotechnol 4, 232 235.
6. Ehrlich, S.D. (1977) Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc Natl Acad Sci USA 70, 1680 1682.
7. Ehrlich, S.D., Niaudet, B. Michel, B. (1982) Use of plasmids from Staphylococcus aureus for cloning of DNA in Bacillus subtilis. Curr Top Microbiol Immunol 96, 19 29.
8. Goeddle, D.V. (1990) Expression in B. subtilis. Meth Enzymol 85, 199 228.
9. Heng, C., Chen, Z., Du, L. Lu, F. (2005) Expression and secretion of an acid-stable a-amylase gene in Bacillus subtilis by SacB promoter and signal peptide. Biotechnol Lett 27, 1731 1736.
10. Itagaki, E., Wakabayashi, T. Hatta, T. (1990) Purification and characterization of 3-ketosteroid-Δ1-dehydrogenase from Nocardia corallina. Biochim Biophys Acta 1038, 60 67.
11. Kaufmann, G., Thole, H., Kraft, R. Atrat, P. (1992) Steroid-1- dehydrogenase of Rhodococcus erythropolis: purification and N-terminal amino acid sequence. J Steroid Biochem Mol Biol 4, 297 301.
12. Lee, J.J., Hahm, K.S., Lee, K.Y. Lee, S.T. (1997) Characterization of an endoxylanase produced by an isolated strain of Bacillus sp. J Microbiol Biotechnol 7, 114 120.
13. Morii, S., Fujii, C., Miyoshi, T., Iwami, M. Itagaki, E. (1998) 3-Ketosteroid-D1-dehydrogenase of Rhodococcus rhodochrous: sequencing of the genomic DNA and hyperexpression, purification, and characterization of the recombinant enzyme. J Biochem 124, 1026 1032.
14. Plesiat, P., Grandguillot, M., Harayama, S., Vragar, S. Michel-Briand, Y. (1991) Cloning, seqencing, and expession of Pseudomonas testosteroni gene encoding 3-oxosteroid Δ1-Dehydrogenase. J Bacteriol 9, 7219 7227.
15. Sambrook, J., Fritsch, E.F. Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. New York : Cold Spring Harbor Laboratory Press.
16. Spizizen, J. (1958) Transformation of biochemically deficient strain of B. subtilis by deoxyribonucleate. Proc Nat Acad Sci USA 44, 1072.
17. Sukhodol'skaya, G.V., Donova, M.V., Nikolaeva, V.M., Koshcheenko, K.A., Dovbnya, D.V., Khomutov, S.M. Gulevskaya, S.A. (2000) Method for Obtaining 1,2-Dehydroderivatives of 4-delta-3-Ketosteroids, RF Patent 2156302.
18. Van Der Geize, R., Hessels, G.I., Van Gerwen, R., Vrijbloed, J.W., Van Der Meijden, P. Dijkhuizen, L. (2000) Targeted Disruption of the kstD Gene Encoding a 3-Ketosteroid-Δ1-Dehydrogenase Isoenzyme of Rhodococcus erythropolis Strain SQ1. Appl Environ Microbiol 5, 2029 2036.
19. Wong, S.L., Kawamura, F. Doi, R.H. (1986) Use of the Bacillus subtilis subtilisin signal peptide for efficient secretion of TEM b-lactamase during growth. J Bacteriol 168, 1005 1009.
20. Ye, R., Kim, J.H., Kim, B.G., Szarka, S., Sihota, E. Wong, S.L. (1999) High level production of intact, biologically active staphylokinase from Bacillus subtilis. Biotechnol Bioeng 62, 87 96.