Conjugated autoxidizable triene (CAT) assay: A novel spectrophotometric method for determination of antioxidant capacity using triacylglycerol as ultraviolet probe

Analytical Biochemistry

Volumn 380, Issue 2, 2008, Pages 282-290

  Laguerre, Mickaël ^a   López Giraldo, Luis J ^a   Lecomte, Jérôme ^a   Baréa, Bruno ^a   Cambon, Emmanuelle ^a   Tchobo, Paul Fidèle ^a   Barouh, Nathalie ^a   Villeneuve, Pierre ^a  

^a UMR IATE   (France)

Author keywords

Antioxidant capacity; Azo initiator; Chain breaker; Conjugated triene; Phenolic compounds; Retarder; Tung oil; Ultraviolet spectrophotometry

Indexed keywords

DRYING OILS; EMULSIFICATION; EMULSIONS; FLAVONOIDS; GLYCEROL; MOLECULES; PHENOLS; PROBES; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY;

ANTIOXIDANT CAPACITY; AZOINITIATOR; CONJUGATED TRIENE; PHENOLIC COMPOUNDS; RETARDER; TUNG OIL; ULTRAVIOLET SPECTROPHOTOMETRY;

ANTIOXIDANTS;

1,1 DIPHENYL 2 PICRYLHYDRAZYL; ANTIOXIDANT; CHLOROGENIC ACID; EPICATECHIN; FATTY ACID; FLUORESCEIN; GALLIC ACID; LINOLEIC ACID; LINOLENIC ACID; OLEIC ACID; OXYGEN RADICAL; PALMITIC ACID; PEROXY RADICAL; PHENOL DERIVATIVE; REACTIVE OXYGEN METABOLITE; ROSMARINIC ACID; STEARIC ACID; TOCOPHEROL; TRIACYLGLYCEROL; TROLOX C; TUNG OIL; VEGETABLE OIL;

ALEURITES; ANALYTIC METHOD; ANTIOXIDANT ACTIVITY; ARTICLE; CHEMICAL STRUCTURE; FATTY ACID ANALYSIS; GAS CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; KINETICS; LIPID OXIDATION; MEASUREMENT; NONHUMAN; OXIDATION; PHYTOCHEMISTRY; PLANT SEED; PRIORITY JOURNAL; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 47749141455     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.06.006     Document Type: Article

References (26)

1. Frankel E.N., Kanner J., and Kinsella J.E. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet 341 (1993) 454-457
2. Hertog M.G.L., Feskens E.J., Hollman P.C., Katan M.B., and Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 342 (1993) 1007-1011
3. Laguerre M., Lecomte J., and Villeneuve P. Evaluation of the ability of antioxidants to counteract lipid oxidation: existing methods, new trends and challenges. Prog. Lipid Res. 46 (2007) 244-282
4. Blois M.S. Antioxidant determination by the use of a stable radical. Nature 181 (1958) 1199-2000
5. Brand-Williams W., Cuvelier M.E., and Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm. Wiss. Technol. 28 (1995) 25-30
6. Ou B., Hampsch-Woodill M., and Prior R.L. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J. Agric. Food Chem. 49 (2001) 4619-4626
7. Tubaro F., Micossi E., and Ursini F. The antioxidant capacity of complex mixtures by kinetic analysis of crocin bleaching inhibition. J. Am. Oil Chem. Soc. 73 (1996) 173-179
8. Naguib Y.M.A. A fluorometric method for measurement of peroxyl radical scavenging activities of lipophilic antioxidants. Anal. Biochem. 265 (1998) 290-298
9. Naguib Y.M.A. Antioxidant activities of astaxanthin and related carotenoids. J. Agric. Food. Chem. 48 (2000) 1150-1154
10. Halliwell B., and Gutteridge J.M. Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol. 186 (1990) 1-85
11. Turon F., Bachain P., Caro Y., Pina M., and Graille J. A direct method for regiospecific analysis of triacylglycerols using alpha-monoacylglycerols. Lipids 37 (2002) 817-821
12. AFNOR, Corps gras d'origine animale et végétale, Détermination de la teneur en tocophérols et tocotriènols: Corps gras, graines oléagineuses, produits dérivés, Vol. 1, Corps gras et produits dérivés, AFNOR, Paris, 1997, pp. 1-10.
13. Brauer R.W., and Steadman L.T. The autoxidation of β-eleostearic acid: the application of the spectrophotometer to the study of the course and the kinetics of the reaction. J. Am. Chem. Soc. 66 (1944) 563-569
14. Holman R.T., Lundberg W.O., and Burr G.O. Spectrophotometric studies of the oxidation of fats: III. Ultraviolet absorption spectra of oxidized octadecatrienoic acids. J. Am. Chem. Soc. 67 (1945) 1390-1394
15. Niki E., Saito T., Kawakami A., and Kamiya Y. Inhibition of methyl linoleate in solution by vitamin E and vitamin C. J. Biol. Chem. 259 (1984) 4177-4182
16. Zhu Q.Y., Huang Y., and Chen Z.-Y. Interaction between flavonoids and α-tocopherol in human low density lipoprotein. J. Nutr. Biochem. 11 (2000) 14-21
17. Yuji H., Weiss J., Villeneuve P., López Giraldo L.J., Figueroa-Espinoza M.-C., and Decker E.A. Ability of surface-active antioxidants to inhibit lipid oxidation in oil-in-water emulsion. J. Agric. Food Chem. 55 (2007) 11052-11056
18. Decker E.A., Warner K., Richards M.P., and Shahidi F. Measuring antioxidant effectiveness in food. J. Agric. Food Chem. 53 (2005) 4303-4310
19. Cao G., Alessio H.M., and Cutler R.G. Oxygen-radical absorbance capacity assay for antioxidants. Free Radic. Biol. Med. 14 (1993) 303-311
20. Villaño D., Fernández-Pachón M.S., Troncoso A.M., and García-Parrilla M.C. Comparison of antioxidant activity of wine phenolic compounds and metabolites in vitro. Anal. Chim. Acta 538 (2005) 391-398
21. Wright J.S., Johnson E.R., and DiLabio G.A. Predicting the activity of phenolic antioxidants: theoretical method, analysis of substituent effects, and application to major families of antioxidants. J. Am. Chem. Soc. 123 (2001) 1173-1183
22. Santos R.B., and Simoes J.A.M. Energetics of the O-H bond in phenol and substituted phenols: a critical evaluation of literature data. J. Phys. Chem. Ref. Data 27 (1998) 707-739
23. Huang D., Ou B., Hampsch-Woodill M., Flanagan J.A., and Prior R.L. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J. Agric. Food Chem. 50 (2002) 4437-4444
24. Porter W., Black E.D., and Drolet A.M. Use of polyamide oxidative fluorescence test on lipid emulsions: contrast in relative effectiveness of antioxidants in bulk versus dispersed systems. J. Agric. Food Chem. 37 (1989) 615-624
25. Frankel E.N., and Meyer A.S. The problems of using one dimensional methods to evaluate multifunctional food and biological antioxidants. J. Sci. Food Agric. 80 (2000) 1925-1941
26. Halliwell B. How to characterize a biological antioxidant. Free Radic. Res. Commun. 9 (1990) 1-32