Ligand-independent assembly of purified soluble magic roundabout (Robo4), a tumor-specific endothelial marker

Protein Expression and Purification

Volumn 61, Issue 1, 2008, Pages 78-82

  Yoshikawa, Mai ^a   Mukai, Yohei ^a   Okada, Yoshiaki ^a   Yoshioka, Yasuo ^a   Tsunoda, Shin ichi ^b   Tsutsumi, Yasuo ^a,b   Okada, Naoki ^a   Aird, William C ^c   Doi, Takefumi ^a   Nakagawa, Shinsaku ^a  

^a OSAKA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF BIOMEDICAL INNOVATION   (Japan)

^c BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

Angiogenesis; Magic roundabout; Multimer; Robo4; Vessel marker

Indexed keywords

CELL SURFACE RECEPTOR; LIGAND; PRIMER DNA; ROBO4 PROTEIN, HUMAN; TUMOR MARKER;

ARTICLE; CELL LINE; GEL CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; NEOVASCULARIZATION (PATHOLOGY); NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLUBILITY; WESTERN BLOTTING;

BASE SEQUENCE; BLOTTING, WESTERN; CELL LINE; CHROMATOGRAPHY, GEL; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; LIGANDS; NEOVASCULARIZATION, PATHOLOGIC; RECEPTORS, CELL SURFACE; SOLUBILITY; TUMOR MARKERS, BIOLOGICAL;

MAMMALIA;

EID: 47749104480     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.05.006     Document Type: Article

References (23)

1. Huminiecki L., and Bicknell R. In silico cloning of novel endothelial-specific genes. Genome Res. 10 (2000) 1796-1806
2. Huminiecki L., Gorn M., Suchting S., Poulsom R., and Bicknell R. Magic roundabout is a new member of the roundabout receptor family that is endothelial specific and expressed at sites of active angiogenesis. Genomics 79 (2002) 547-552
3. Andrews W., Barber M., Hernadez-Miranda L.R., Xian J., Rakic S., Sundaresan V., Rabbitts T.H., Pannell R., Rabbitts P., Thompson H., Erskine L., Murakami F., and Parnavelas J.G. The role of Slit-Robo signaling in the generation migration, and morphological differentiation of cortical interneurons. Dev. Biol. 313 (2008) 648-658
4. Challa A.K., Beattie C.E., and Seeger M.A. Identification and characterization of roundabout orthologs in zebrafish. Mech. Dev. 101 (2001) 249-253
5. Bedell V.M., Yeo S.Y., Park K.W., Chung J., Seth P., Shivalingappa V., Zhao J., Obara T., Sukhatme V.P., Drummond I.A., Li D.Y., and Ramchandran R. Roundabout4 is essential for angiogenesis in vivo. Proc. Natl. Acad. Sci. USA 102 (2005) 6373-6378
6. Grone J., Doebler O., Loddenkemper C., Hotz B., Buhr H.J., and Bhargava S. Robo1/Robo4: differential expression of angiogenic markers in colorectal cancer. Oncol. Rep. 15 (2006) 1437-1443
7. Suchting S., Heal P., Tahtis K., Stewart L.M., and Bicknell R. Soluble Robo4 receptor inhibits in vivo angiogenesis and endothelial cell migration. FASEB J. 19 (2005) 121-123
8. Seth P., Lin Y., Hanai J., Shivalingappa V., Duyao M.P., and Sukhatme V.P. Magic roundabout a tumor endothelial marker: expression and signaling. Biochem. Biophys. Res. Commun. 332 (2005) 533-541
9. Park K.W., Morrison C.M., Sorensen L.K., Jones C.A., Rao Y., Chien C.B., Wu J.Y., Urness L.D., and Li D.Y. Robo4 is a vascular-specific receptor that inhibits endothelial migration. Dev. Biol. 261 (2003) 251-267
10. Wang B., Xiao Y., Ding B.B., Zhang N., Yuan X., Gui L., Qian K.X., Duan S., Chen Z., Rao Y., and Geng J.G. Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity. Cancer Cell 4 (2003) 19-29
11. Gorn M., Anige M., Burkholder I., Muller B., Scheffler A., Edler L., Boeters I., Panse J., Schuch G., Hossfeld D.K., and Laack E. Serum levels of Magic Roundabout protein in patients with advanced non-small cell lung cancer (NSCLC). Lung Cancer 49 (2005) 71-76
12. Yuan W., Zhou L., Chen J.H., Wu J.Y., Rao Y., and Ornitz D.M. The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance. Dev. Biol. 212 (1999) 290-306
13. Fujiwara M., Ghazizadeh M., and Kawanami O. Potential role of the Slit/Robo signal pathway in angiogenesis. Vasc. Med. 11 (2006) 115-121
14. Legg J.A., Herbert J.M., Clissold P., and Bicknell R. Slits and roundabouts in cancer, tumour angiogenesis and endothelial cell migration. Angiogenesis (2008)
15. Morlot C., Thielens N.M., Ravelli R.B., Hemrika W., Romijn R.A., Gros P., Cusack S., and McCarthy A.A. Structural insights into the Slit-Robo complex. Proc. Natl. Acad. Sci. USA 104 (2007) 14923-14928
16. Kaur S., Castellone M.D., Bedell V.M., Konar M., Gutkind J.S., and Ramchandran R. Robo4 signaling in endothelial cells implies attraction guidance mechanisms. J. Biol. Chem. 281 (2006) 11347-11356
17. Liu C., Dalby B., Chen W., Kilzer J.M., and Chiou H.C. Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells. Mol. Biotechnol. (2008)
18. P. Hawley-Nelson, V. Ciccarone, M.L. Moore, Transfection of cultured eukaryotic cells using cationic lipid reagents, in: F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl (Eds.), Current Protocols in Molecular Biology, John Wiley & Sons, Inc., New York, 2008 (Chapter 9, Unit 9.4).
19. Werner R.G., Noe W., Kopp K., and Schluter M. Appropriate mammalian expression systems for biopharmaceuticals. Arzneimittelforschung 48 (1998) 870-880
20. Werner R.G., Kopp K., and Schlueter M. Glycosylation of therapeutic proteins in different production systems. Acta Paediatr. Suppl. 96 (2007) 17-22
21. Gaudry J.P., Arod C., Sauvage C., Busso S., Dupraz P., Pankiewicz R., and Antonsson B. Purification of the extracellular domain of the membrane protein GlialCAM expressed in HEK and CHO cells and comparison of the glycosylation. Protein Expr. Purif. 58 (2008) 94-102
22. Ruch C., Skiniotis G., Steinmetz M.O., Walz T., and Ballmer-Hofer K. Structure of a VEGF-VEGF receptor complex determined by electron microscopy. Nat. Struct. Mol. Biol. 14 (2007) 249-250
23. Mac Gabhann F., and Popel A.S. Dimerization of VEGF receptors and implications for signal transduction: a computational study. Biophys. Chem. 128 (2007) 125-139