Transfection of cultured eukaryotic cells using cationic lipid reagents

Current Protocols in Molecular Biology

Volumn , Issue SUPPL. 81, 2008, Pages

  Hawley Nelson, Pamela ^a   Ciccarone, Valentina ^b   Moore, Meredith L ^c  

^a CENTOCOR INC   (United States)

^b MacroGenics Inc   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Cationic lipid; Cell culture; Luciferase; Stable gene expression; Tansfection; Transgene; Transient gene expression

Indexed keywords

CATIONIC LIPID; CELL DNA; LIPID; REAGENT; SINGLE STRANDED RNA; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; VIRUS DNA; CATION; DNA; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; LIPOFECTAMINE; 1,2 DIOLEOYL 3 TRIMETHYLAMMONIOPROPANE; ANTIBIOTIC G 418; CATIONIC LIPID REAGENT;

ARTICLE; CELL ADHESION; CELL CULTURE; CELL GROWTH; CELL LINEAGE; CELL SUSPENSION; EUKARYOTIC CELL; GENE EXPRESSION; GENE TECHNOLOGY; GENE THERAPY; GENETIC TRANSFECTION; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; ANIMAL; BACULOVIRUS; CELL STRAIN 3T3; CHEMISTRY; CYTOLOGY; HUMAN; INSECT; LEUKEMIA CELL LINE; METABOLISM; METHODOLOGY; MOUSE; BACULOVIRIDAE; CELL CULTURE TECHNIQUE; CLINICAL PROTOCOL; COMPLEX FORMATION; DNA TRANSFECTION; GENE SILENCING; INCUBATION TEMPERATURE; INCUBATION TIME; MOLECULAR STABILITY; PROCESS OPTIMIZATION; PROTEIN SYNTHESIS; RNA TRANSFECTION; SCALE UP; SURFACE AREA;

EUKARYOTA; HEXAPODA;

ANIMALS; BACULOVIRIDAE; CATIONS; CELL ADHESION; CELLS, CULTURED; DNA; EUKARYOTIC CELLS; HUMANS; INDICATORS AND REAGENTS; INSECTS; JURKAT CELLS; LIPIDS; MICE; NIH 3T3 CELLS; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 39049090083     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb0904s81     Document Type: Article

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