Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry

Biomedical Chromatography

Volumn 20, Issue 5, 2006, Pages 429-433

  Deventer, Koen ^a   Van Eenoo, P ^a   Delbeke, F T ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

17 trenbolone; 3 hydroxystanozolol; Anabolic steroids; Doping analysis; Gestrinone; Stanozolol

Indexed keywords

ENZYMATIC HYDROLYSIS; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIPIDS; MASS SPECTROMETERS; MASS SPECTROMETRY;

17Α-TRENBOLONE; 3′-HYDROXYSTANOZOLOL; ANABOLIC STEROID; DOPING ANALYSIS; ELECTROSPRAY ION TRAP; GESTRINONE; ION-TRAP MASS SPECTROMETRY; LIQUID CHROMATOGRAPHY ELECTROSPRAY; STANOZOLOL; TRENBOLONE;

ELECTROSPRAY IONIZATION;

17ALPHA TRENBOLONE; 3' HYDROXYSTANOZOLOL; ANABOLIC AGENT; ETHER; FLUOXYMESTERONE; GESTRINONE; SEX HORMONE; TETRAHYDROGESTRINONE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DOPING; DRUG DETERMINATION; ELECTROSPRAY MASS SPECTROMETRY; GAS CHROMATOGRAPHY; HUMAN; HYDROLYSIS; ION TRAP MASS SPECTROMETRY; LIQUID CHROMATOGRAPHY; LIQUID LIQUID EXTRACTION; MASS SPECTROMETRY; SCREENING TEST; URINALYSIS; VALIDATION PROCESS;

EID: 33646767075     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.579     Document Type: Article

References (15)

1. Ayotte C, Goudreault D and Charlebois A. Testing for natural and synthetic anabolic agents in human urine. Journal of Chromatography B 1996; 687: 3.
2. Bruins AP. Mechanistic aspects of electrospray ionization. Journal of Chromatography A 1998; 794: 345.
3. Catlin DH, Sekera MH, Ahrens BD, Starcevic B, Chang YC and Hatton CK. Tetrahydrogestrinone: discovery, synthesis and detection in urine. Rapid Communications in Mass Spectrometry 2004; 18: 1245.
4. Choi MH, Chung BC, Lee W, Lee UC and Kim Y. Determination of anabolic steroids by gas chromatography negative-ion chemical ionization mass spectrometry and gas chromatography negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization. Rapid Communications in Mass. Spectrometry 1999; 13: 376.
5. Deventer K, Van Eenoo P, Mikulčíková P, Van Thuyne W and Delbeke FT. Quantitative analysis of androst-4-ene-3,6,17-trione and metabolites in human urine after the administration of a food supplement by liquid chromatography/ion trap-mass spectrometry. Journal of Chromatography B (in press).
6. Eurachem Working Group. A Laboratory Guide to Method Validation and Related Topics. Eurachem: Teddington, 1998.
7. Guan F, Uboh C, Soma L, Hess A, Lu Y and Tang DS. Sensitive liquid chromatographic/tandem mass spectrometric method for the determination of beclomethasone dipropionate and its metabolites in equine plasma and urine. Journal of Mass Spectrometry 2003; 38: 823.
8. Leinonen A, Kuuranne T and Kostiainen R. Liquid chromatography/mass spectrometry in anabolic steroid analysis-optimization and comparison of three ionization techniques: electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization. Journal of Mass Spectrometry 2002; 37: 693.
9. Leinonen A, Kuuranne T, Kotiaho T and Kostiainen R. Screening of free 17-alkyl-substituted anabolic steroids in human urine by liquid chromatography-electrospray ionization tandem mass spectrometry. Steroids 2004; 69: 101-109.
10. Saugy M, Cardis C, Robinson N and Schweizer C. Test methods: anabolics. Best Practice and Research Clinical Endocrinology and Metabolism 2000; 14: 111.
11. Schänzer W and Donike M. Metabolism of anabolic steroids in man: synthesis and use of reference substances for identification of anabolic steroid metabolites. Analytica Chimica Acta 1993; 275: 23.
12. Schänzer W, Opfermann G and Donike M. Metabolism of stanozolol:identification and synthesis of urinary metabolites. Journal of Steroid Biochemistry 1990; 36: 153.
13. Van de Wiele M, De Wasch K, Vercammen J, Courtheyn D, de Brabander H and Impens S. Determination of 16β-hydroxystanozolol in urine and faeces by liquid chromatography-multiple mass spectrometry. Journal of Chromatography A 2000; 964: 205.
14. WADA. The World Anti-doping Code. Technical document-TD2004MRPL. WADA, 2004.
15. WADA. The World Anti-doping Code, The 2005 Prohibited List. International Standard. WADA, 2005.