Immunofluorometric activity-based probe analysis of active KLK6 in biological fluids

Biological Chemistry

Volumn 389, Issue 6, 2008, Pages 747-756

  Oikonomopoulou, Katerina ^a,d   Hansen, Kristina K ^b   Baruch, Amos ^c   Hollenberg, Morley D ^b   Diamandis, Eleftherios P ^a,d  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF CALGARY   (Canada)

^c KAI Pharmaceuticals   (United States)

^d UNIVERSITY OF TORONTO   (Canada)

Author keywords

Activity based probes; Ascites fluid; Cerebrospinal fluid; Human kallikrein related peptidases; Serine proteinases; Trypsin like activity

Indexed keywords

BIOLOGICAL MARKER; ENZYME PRECURSOR; KALLIKREIN 6; THERMOLYSIN;

ASCITES FLUID ANALYSIS; BODY FLUID; CANCER CELL CULTURE; CEREBROSPINAL FLUID ANALYSIS; CONFERENCE PAPER; DISEASE MARKER; ENZYME ACTIVITY; IMMUNOASSAY; IMMUNOREACTIVITY; OVARY CANCER; PRIORITY JOURNAL; PROTEIN PURIFICATION; QUANTITATIVE STUDY; SUPERNATANT;

ASCITES; BODY FLUIDS; CELL LINE, TUMOR; ENZYME ACTIVATION; ENZYME PRECURSORS; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; KALLIKREINS; STAINING AND LABELING; THERMOLYSIN;

EID: 47549118861     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC.2008.086     Document Type: Conference Paper

References (56)

1. Anisowicz, A., Sotiropoulou, G., Stenman, G., Mok, S.C., and Sager, R. (1996). A novel protease homolog differentially expressed in breast and ovarian cancer. Mol. Med. 2, 624-636.
2. Baruch, A., Jeffery, D.A., and Bogyo, M. (2004). Enzyme activity - it's all about image. Trends Cell Biol. 14, 29-35.
3. Bayes, A., Tsetsenis, T., Ventura, S., Vendrell, J., Aviles, F.X., and Sotiropoulou, G. (2004). Human kallikrein 6 activity is regulated via an autoproteolytic mechanism of activation/inactivation. Biol. Chem. 385, 517-524.
4. Bernett, M.J., Blaber, S.I., Scarisbrick, I.A., Dhanarajan, P., Thompson, S.M., and Blaber, M. (2002). Crystal structure and biochemical characterization of human kallikrein 6 reveals that a trypsin-like kallikrein is expressed in the central nervous system. J. Biol. Chem. 277, 24562-24570.
5. Blaber, S.I., Scarisbrick, I.A., Bernett, M.J., Dhanarajan, P., Seavy, M.A., Jin, Y., Schwartz, M.A., Rodriguez, M., and Blaber, M. (2002). Enzymatic properties of rat myelencephalon-specific protease. Biochemistry 41, 1165-1173.
6. Blaber, S.I., Ciric, B., Christophi, G.P., Bernett, M.J., Blaber, M., Rodriguez, M., and Scarisbrick, I.A. (2004). Targeting kallikrein 6 proteolysis attenuates CNS inflammatory disease. FASEB J. 18, 920-922.
7. Blaber, S.I., Yoon, H., Scarisbrick, I.A., Juliano, M.A., and Blaber, M. (2007). The autolytic regulation of human kallikrein-related peptidase 6. Biochemistry 46, 5209-5217.
8. Borgoño, C.A. and Diamandis, E.P. (2004). The emerging roles of human tissue kallikreins in cancer. Nat. Rev. Cancer 4, 876-890.
9. Borgoño, C.A., Michael, I.P., and Diamandis, E.P. (2004). Human tissue kallikreins: physiologic roles and applications in cancer. Mol. Cancer Res. 2, 257-280.
10. Christopoulos, T.K. and Diamandis, E.P. (1992). Enzymatically amplified time-resolved fluorescence immunoassay with terbium chelates. Anal. Chem. 64, 342-346.
11. Diamandis, E.P., Yousef, G.M., Luo, L.Y., Magklara, A., and Obiezu, C.V. (2000a). The new human kallikrein gene family: implications in carcinogenesis. Trends Endocrinol. Metab. 11, 54-60.
12. Diamandis, E.P., Yousef, G.M., Petraki, C., and Soosaipillai, A.R. (2000b). Human kallikrein 6 as a biomarker of Alzheimer's disease. Clin. Biochem. 33, 663-667.
13. Diamandis, E.P., Yousef, G.M., Soosaipillai, A.R., Grass, L., Porter, A., Little, S., and Sotiropoulou, G. (2000c). Immunofluorometric assay of human kallikrein 6 (zyme/protease M/neurosin) and preliminary clinical applications. Clin. Biochem. 33, 369-375.
14. Dumont, D., Noben, J.P., Raus, J., Stinissen, P., and Robben, J. (2004). Proteomic analysis of cerebrospinal fluid from multiple sclerosis patients. Proteomics 4, 2117-2124.
15. Ferguson, R.A., Yu, H., Kalyvas, M., Zammit, S., and Diamandis, E.P. (1996). Ultrasensitive detection of prostate specific antigen by a time-resolved immunofluorometric assay and the Immulite® immunochemiluminescent third generation assay: potential applications in prostate and breast cancers. Clin. Chem. 42, 675-684.
16. Gomis-Ruth, F.X., Bayes, A., Sotiropoulou, G., Pampalakis, G., Tsetsenis, T., Villegas, V., Avilés, F.X., and Coll, M. (2002). The structure of human prokallikrein 6 reveals a novel activation mechanism for the kallikrein family. J. Biol. Chem. 277, 27273-27281.
17. Hansen, K.K., Sherman, P.M., Cellars, L., Andrade-Gordon, P., Pan, Z., Baruch, A., Wallace, J.L., Hollenberg, M.D., and Vergnolle, N. (2005). A major role for proteolytic activity and proteinase-activated receptor-2 in the pathogenesis of infectious colitis. Proc. Natl. Acad. Sci. USA 102, 8363-8368.
18. Hansen, K.K., Oikonomopoulou, K., Li, Y., and Hollenberg, M.D. (2007). Proteinases, proteinase-activated receptors (PARs) and the pathophysiology of cancer and diseases of the cardiovascular, musculoskeletal, nervous and gastrointestinal systems. Naunyn-Schmiedeberg's Arch. Pharmacol. In press, doi: 10.1007/s00210-007-0194-2.
19. Heisler, L.E., Evangelou, A., Lew, A.M., Trachtenberg, J., Elsholtz, H.P., and Brown, T.J. (1997). Androgen-dependent cell cycle arrest and apoptotic death in PC-3 prostatic cell cultures expressing a full-length human androgen receptor. Mol. Cell. Endocrinol. 126, 59-73.
20. Hollenberg, M.D., Oikonomopoulou, K., Hansen, K.K., Saifeddine, M., Rithwik Ramachandran, R., and Diamandis, E.P. (2007). Kallikreins and proteinase-mediated signaling: proteinase-activated receptors (PARs) and the pathophysiology of inflammatory diseases and cancer. Biol. Chem. 389, 643-651.
21. 1-antichymotrypsin. Clin. Chem. 49, 746-751.
22. Iwata, A., Maruyama, M., Akagi, T., Hashikawa, T., Kanazawa, I., Tsuji, S., and Nukina, N. (2003). Alpha-synuclein degradation by serine protease neurosin: implication for pathogenesis of synucleinopathies. Hum. Mol. Genet. 12, 2625-2635.
23. Kuramitsu, Y. and Nakamura, K. (2006). Proteomic analysis of cancer tissues: shedding light on carcinogenesis and possible biomarkers. Proteomics 6, 5650-5661.
24. Law, R.H., Zhang, Q., McGowan, S., Buckle, A.M., Silverman, G.A., Wong, W., Rosado, C.J., Langendorf, C.G., Pike, R.N., Bird, P.I., and Whisstock, J.C. (2006). An overview of the serpin superfamily. Genome Biol. 7, 216.
25. Little, S.P., Dixon, E.P., Norris, F., Buckley, W., Becker, G.W., Johnson, M., Dobbins, J.R., Wyrick, T., Miller, J.R., MacKellar, W., et al. (1997). Zyme, a novel and potentially amyloidogenic enzyme cDNA isolated from Alzheimer's disease brain. J. Biol. Chem. 272, 25135-25142.
26. Luo, L.Y. and Jiang, W. (2006). Inhibition profiles of human tissue kallikreins by serine protease inhibitors. Biol. Chem. 387, 813-816.
27. Luo, L.Y., Soosaipillai, A., Grass, L., and Diamandis, E.P. (2006) Characterization of human kallikreins 6 and 10 in ascites fluid from ovarian cancer patients. Tumour Biol. 27, 227-234.
28. Magklara, A., Mellati, A.A., Wasney, G.A., Little, S.P., Sotiropoulou, G., Becker, G.W., and Diamandis, E.P. (2003). Characterization of the enzymatic activity of human kallikrein 6: autoactivation, substrate specificity, and regulation by inhibitors. Biochem. Biophys. Res. Commun. 307, 948-955.
29. Matsui, H., Kimura, A., Yamashiki, N., Moriyama, A., Kaya, M., Yoshida, I., Takagi, N., and Takahashi, T. (2000). Molecular and biochemical characterization of a serine proteinase predominantly expressed in the medulla oblongata and cerebellar white matter of mouse brain. J. Biol. Chem. 275, 11050-11057.
30. Meier, N., Dear, T.N., and Boehm, T. (1999). A novel serine protease overexpressed in the hair follicles of nude mice. Biochem. Biophys. Res. Commun. 258, 374-378.
31. Mitsui, S., Okui, A., Uemura, H., Mizuno, T., Yamada, T., Yamamura, Y., and Yamaguchi, N. (2002). Decreased cerebrospinal fluid levels of neurosin (KLK6), an aging-related protease, as a possible new risk factor for Alzheimer's disease. Ann. NY Acad. Sci. 977, 216-223.
32. Nagahara, H., Mimori, K., Utsunomiya, T., Barnard, G.F., Ohira, M., Hirakawa, K., and Mori, M. (2005). Clinicopathologic and biological significance of kallikrein 6 overexpression in human gastric cancer. Clin. Cancer Res. 11, 6800-6806.
33. Ogawa, K., Yamada, T., Tsujioka, Y., Taguchi, J., Takahashi, M., Tsuboi, Y., Fujino, Y., Nakajima, M., Yamamoto, T., Akatsu, H., Mitsui, S., and Yamaguchi, N. (2000). Localization of a novel type trypsin-like serine protease, neurosin, in brain tissues of Alzheimer's disease and Parkinson's disease. Psychiatry Clin. Neurosci. 54, 419-426.
34. Oikonomopoulou, K., Hansen, K.K., Saifeddine, M., Tea, I., Blaber, M., Blaber, S.I., Scarisbrick, I., Andrade-Gordon, P., Cottrell, G.S., Bunnett, N.W., et al. (2006a). Proteinase-activated receptors, targets for kallikrein signaling. J. Biol. Chem. 281, 32095-32112.
35. Oikonomopoulou, K., Hansen, K.K., Saifeddine, M., Vergnolle, N., Tea, I., Blaber, M., Blaber, S.I., Scarisbrick, I., Diamandis, E.P., and Hollenberg, M.D. (2006b). Kallikrein-mediated cell signalling: targeting proteinase-activated receptors (PARs). Biol. Chem. 387, 817-824.
36. Oikonomopoulou, K., Hansen, K.K., Saifeddine, M., Vergnolle, N., Tea, I., Diamandis, E.P., and Hollenberg, M.D. (2006c) Proteinase-mediated cell signalling: targeting proteinase-activated receptors (PARs) by kallikreins and more. Biol. Chem. 387, 677-685.
37. Okui, A., Kominami, K., Uemura, H., Mitsui, S., and Yamaguchi, N. (2001). Characterization of a brain-related serine protease, neurosin (human kallikrein 6), in human cerebrospinal fluid. Neuroreport 12, 1345-1350.
38. Ossovskaya, V.S. and Bunnett, N.W. (2004). Protease-activated receptors: contribution to physiology and disease. Physiol. Rev. 84, 579-621.
39. Pan, Z., Jeffery, D.A., Chehade, K., Beltman, J., Clark, J.M., Grothaus, P., Bogyo, M., and Baruch, A. (2006). Development of activity-based probes for trypsin-family serine proteases. Bioorg. Med. Chem. Lett. 16, 2882-2885.
40. Petraki, C.D., Karavana, V.N., Revelos, K.I., Luo, L.Y., and Diamandis, E.P. (2002). Immunohistochemical localization of human kallikreins 6 and 10 in pancreatic islets. Histochem. J. 34, 313-322.
41. Petraki, C.D., Karavana, V.N., Skoufogiannis, P.T., Little, S.P., Howarth, D.J., Yousef, G.M., and Diamandis, E.P. (2001). The spectrum of human kallikrein 6 (zyme/protease M/neurosin) expression in human tissues as assessed by immunohistochemistry. J. Histochem. Cytochem. 49, 1431-1441.
42. Ramachandran, R. and Hollenberg M.D. (2008) Proteinases and signalling: pathophysiological and therapeutic implications via PARs and more. Br. J. Pharmacol. 153, S263-S282.
43. Sadaghiani, A.M., Verhelst, S.H., and Bogyo, M. (2007). Tagging and detection strategies for activity-based proteomics. Curr. Opin. Chem. Biol. 11, 20-28.
44. Sauter, E.R., Lininger, J., Magklara, A., Hewett, J.E., and Diamandis, E.P. (2004). Association of kallikrein expression in nipple aspirate fluid with breast cancer risk. Int. J. Cancer 108, 588-591.
45. Scarisbrick, I.A., Blaber, S.I., Lucchinetti, C.F., Genain, C.P., Blaber, M., and Rodriguez, M. (2002). Activity of a newly identified serine protease in CNS demyelination. Brain 125, 1283-1296.
46. Scarisbrick, I.A., Towner, M.D., and Isackson, P.J. (1997). Nervous system-specific expression of a novel serine protease: regulation in the adult rat spinal cord by excitotoxic injury. J. Neurosci. 17, 8156-8168.
47. Shaw, J.L. and Diamandis, E.P. (2007). Distribution of 15 human kallikreins in tissues and biological fluids. Clin. Chem. 53, 1423-1432.
48. Shaw, J.L., Smith, C.R., and Diamandis, E.P. (2007). Proteomic analysis of human cervico-vaginal fluid. J. Proteome Res. 6, 2859-2865.
49. Shimizu-Okabe, C., Yousef, G.M., Diamandis, E.P., Yoshida, S., Shiosaka, S., and Fahnestock, M. (2001). Expression of the kallikrein gene family in normal and Alzheimer's disease brain. Neuroreport 12, 2747-2751.
50. Steinhoff, M., Buddenkotte, J., Shpacovitch, V., Rattenholl, A., Moormann, C., Vergnolle, N., Luger, T.A., and Hollenberg, M.D. (2005). Proteinase-activated receptors: transducers of proteinase-mediated signaling in inflammation and immune response. Endocr. Rev. 26, 1-43.
51. Yamashiro, K., Tsuruoka, N., Kodama, S., Tsujimoto, M., Yamamura, Y., Tanaka, T., Nakazato, H., and Yamaguchi, N. (1997). Molecular cloning of a novel trypsin-like serine protease (neurosin) preferentially expressed in brain. Biochim. Biophys. Acta 1350, 11-14.
52. Yousef, G.M., Obiezu, C.V., Luo, L.Y., Magklara, A., Borgoño, C.A., Kishi, T., Memari, N., Michael, P., Sidiropoulos, M., Kurlender, L., et al. (2005). Human tissue kallikreins: from gene structure to function and clinical applications. Adv. Clin. Chem. 39, 11-79.
53. Yousef, G.M., Borgoño, C.A., Popalis, C., Yacoub, G.M., Polymeris, M.E., Soosaipillai, A., and Diamandis, E.P. (2004). Insilico analysis of kallikrein gene expression in pancreatic and colon cancers. Anticancer Res. 24, 43-51.
54. Yousef, G.M., Luo, L.Y., Scherer, S.W., Sotiropoulou, G., and Diamandis, E.P. (1999). Molecular characterization of Zyme/Protease M/Neurosin (PRSS9), a hormonally regulated kallikrein-like serine protease. Genomics 62, 251-259.
55. Yuan, X. and Desiderio, D.M. (2005). Proteomics analysis of prefractionated human lumbar cerebrospinal fluid. Proteomics 5, 541-550.
56. Zarghooni, M., Soosaipillai, A., Grass, L., Scorilas, A., Mirazimi, N., and Diamandis, E.P. (2002). Decreased concentration of human kallikrein 6 in brain extracts of Alzheimer's disease patients. Clin. Biochem. 35, 225-231.