Calculation of the isoelectric point of tryptic peptides in the pH 3.5-4.5 range based on adjacent amino acid effects

Electrophoresis

Volumn 29, Issue 13, 2008, Pages 2768-2778

  Cargile, Benjamin J ^a   Sevinsky, Joel R ^a   Essader, Amal S ^a   Eu, Jerry P ^b   Stephenson, James L ^a  

^a RTI INTERNATIONAL   (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Immobilized pH gradient; Isoelectric focusing; Mass spectrometry; Peptide identification; pI calculation

Indexed keywords

AMINO ACIDS; CELL CULTURE; ELECTROPHORESIS; PEPTIDES; SEARCH ENGINES;

AMINO-ACIDS; IMMOBILIZED PH GRADIENT; ISO-ELECTRIC POINTS; ISOELECTRIC FOCUSING; PEPTIDE IDENTIFICATION; PH GRADIENTS; PI CALCULATION; PI VALUES; RANGE-BASED; TRYPTIC PEPTIDES;

MASS SPECTROMETRY;

AMINO ACID; ASPARTIC ACID; GLUTAMIC ACID; PEPTIDE;

ACCURACY; ANALYTICAL ERROR; ANIMAL TISSUE; ARTICLE; BREAST CANCER; CALCULATION; CANCER CELL CULTURE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOSOL; GENE EXPRESSION; GENETIC ALGORITHM; GENETIC ANALYSIS; HUMAN; HUMAN CELL; HUMAN TISSUE; ISOELECTRIC POINT; LIQUID CHROMATOGRAPHY; LUNG PARENCHYMA; MASS SPECTROMETRY; NONHUMAN; PEPTIDE ANALYSIS; PH MEASUREMENT; RAT; SINGLE NUCLEOTIDE POLYMORPHISM; VALIDATION PROCESS;

EID: 47549117421     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200700701     Document Type: Article

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