Viability of porcine corneal epithelium ex vivo and effect of exposure to air: A pilot study for a dry eye model

Cornea

Volumn 23, Issue 7, 2004, Pages 715-719

  Choy, Emily Pik Yin ^a   To, Tony Shing Shun ^a   Cho, Pauline ^a   Benzie, Iris Frances Forster ^a   Choy, Camus Kar Man ^a  

^a HONG KONG POLYTECHNIC UNIVERSITY   (Hong Kong)

Author keywords

Cornea; Dry eye syndrome; Exposure keratitis; Porcine corneal epithelium viability

Indexed keywords

BUFFER; PHOSPHATE; SODIUM CHLORIDE;

AMBIENT AIR; ANIMAL TISSUE; ARTICLE; CELL DAMAGE; CELL VIABILITY; CONTROLLED STUDY; CORNEA EPITHELIUM; DISEASE MODEL; DRY EYE; ENUCLEATION; EX VIVO STUDY; EXPOSURE; EXPOSURE KERATITIS; KERATITIS; NONHUMAN; PILOT STUDY; PRIORITY JOURNAL; SWINE;

AIR; ANIMALS; CELL DEATH; DISEASE MODELS, ANIMAL; DRY EYE SYNDROMES; EPITHELIUM, CORNEAL; EYE ENUCLEATION; KERATITIS; PILOT PROJECTS; SWINE; TIME FACTORS; TISSUE SURVIVAL;

EID: 4744360311     PISSN: 02773740     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.ico.0000127475.29551.56     Document Type: Article

References (45)

1. Bandeen-Roche K, Munoz B, Tielsch JM, et al. Self-reported assessment of dry eye in a population-based setting. Invest Ophthalmol Vis Sci. 1997;38:2469-2475.
2. Schein O, Munoz B, Tielsch J, et al. Prevalence of dry eye among the elderly. Am J Ophthalmol. 1997;124:723-728.
3. Baudouin C. The pathology of dry eye. Surv Ophthalmol. 2001;45(Suppl 2):S211-S220.
4. Stern ME, Beuerman RW, Fox RI, et al. The pathology of dry eye: the interaction between the ocular surface and lacrimal glands. Cornea. 1998;17:584-589.
5. Friedlaender MH. Ocular manifestations of Sjogren's syndrome: keratoconjunctivitis sicca. Rheum Dis Clin North Am. 1992;18:591-608.
6. Terry MA. Dry eye in the elderly. Drugs Aging. 2001;18:101-107.
7. Virtanen T, Konttinen YT, Honkanen N, et al. Tear fluid plasmin activity of dry eye patients with Sjogren's syndrome. Acta Ophthalmol Scand. 1997;75:137-141.
8. Dursun D, Wang M, Monroy D, et al. A mouse model of keratoconjunctivitis sicca. Invest Ophthalmol Vis Sci. 2002;43:632-638.
9. Fujihara T, Murakami T, Fujita H, et al. Improvement of corneal barrier function by the P2Y(2) agonist INS365 in a rat dry eye model. Invest Ophthalmol Vis Sci. 2001;42:96-100.
10. Burgalassi S, Panichi L, Chetoni P, et al. Development of a simple dry eye model in the albino rabbit and evaluation of some tear substitutes. Ophthalmic Res. 1999;31:229-235.
11. Moore CP, McHugh JB, Thorne JG, et al. Effect of cyclosporine on conjunctival mucin in a canine keratoconjunctivitis sicca model. Invest Ophthalmol Vis Sci. 2001;42:653-659.
12. Hicks SJ, Corfield AP, Kaswan RL, et al. Biochemical analysis of ocular surface mucin abnormalities in dry eye: the canine model. Exp Eye Res. 1998;67:709-718.
13. Kaswan RL, Salisbury MA, Ward DA. Spontaneous canine keratoconjunctivitis sicca. A useful model for human keratoconjunctivitis sicca: treatment with cyclosporine eye drops. Arch Ophthalmol. 1989;107:1210-1216.
14. Gilbard JP, Rossi SR, Gray KL, et al. Tear film osmolarity and ocular surface disease in two rabbit models for keratoconjunctivitis sicca. Invest Ophthalmol Vis Sci. 1988;29:374-378:
15. Fujihara T, Nagano T, Nakamura M, et al. Lactoferrin suppresses loss of corneal epithelial integrity in a rabbit short-term dry eye model. J Ocul Pharmacol Ther. 1998;14:99-107.
16. Driot JY, Bonne C. Beneficial effects of a retinoic acid analog, CBS-211 A, on an experimental model of keratoconjunctivitis sicca. Invest Ophthalmol Vis Sci. 1992;33:190-195.
17. Gilbard JP, Rossi SR. An electrolyte-based solution that increases corneal glycogen and conjunctival goblet-cell density: in a rabbit model for keratoconjunctivitis sicca. Ophthalmology. 1992;99:600-604.
18. Johnson BW, Whiteley HE, McLaughlin SA. Effects of inflammation and aqueous tear film deficiency on conjunctival morphology and ocular mucus composition in cats. Am J Vet Res. 1990;51:820-824.
19. Maitchouk DY, Beuerman RW, Ohta T, et al. Tear production after unilateral removal of the main lacrimal gland in squirrel monkeys. Arch Ophthalmol. 2000;118:246-252.
20. Maurice D. The effect of the low blink rate in rabbits on topical drug penetration. J Ocul Pharmacol Ther. 1995;11:297-304.
21. Bentivoglio AR, Bressman SB, Cassetta E, et al. Analysis of blink rate patterns in normal subjects. Mov Disord. 1997;12:1028-1034.
22. Khurana AK, Chaudhary R, Ahluwalia BK, et al. Tear film profile in dry eye. Acta Ophthalmol (Copenh). 1991;69:79-86.
23. Kuiper B, Boeve MH, Jansen T, et al. Ophthalmologic examination in systemic toxicity studies: an overview. Lab Anim. 1997;31:177-183.
24. Holly FJ, Lemp MA. Tear physiology and dry eyes. Sum Ophthalmol. 1977;22:69-87.
25. Greiner JV, Kopp SJ, Gillette TE, et al. Phosphatic metabolites of the intact cornea by phosphorus-31 nuclear magnetic resonance. Invest Ophthalmol Vis Sci. 1983;24:535-542.
26. He YG, McCulley JP, Alizadeh H, et al. A pig model of Acanthamoeba keratitis: transmission via contaminated contact lenses. Invest Ophthalmol Vis Sci. 1992;33:126-133.
27. Zhang Z, Monteiro-Riviere NA. Comparison of integrins in human skin, pig skin, and perfused skin: an in vitro skin toxicology model. J Appl Toxicol. 1997;17:247-253.
28. Christie KN, Thomson C, Hopwood D. A comparison of membrane enzymes of human and pig oesophagus; the pig oesophagus is a good model for studies of the gullet in man. Histochem J. 1995;27:231-239.
29. King JR, Riviere JE, Monteiro-Riviere NA. Characterization of lewisite toxicity in isolated perfused skin. Toxicol Appl Pharmacol. 1992;116:189-201.
30. Wang TT, Reisenauer AM, Halsted CH. Comparison of folate conjugase activities in human, pig, rat and monkey intestine. J Nutr. 1985;115:814-819.
31. Camber O. An in vitro model for determination of drug permeability through the cornea. Acta Pharm Suec. 1985;22:335-342.
32. 31P nuclear magnetic resonance spectroscopic study of intact tissues. Cornea. 1993;12:461-465.
33. Kampmeier J, Radt B, Birngruber R, et al. Thermal and biomechanical parameters of porcine cornea. Cornea. 2000;19:355-363.
34. Gilbard JP. Tear film osmolarity and keratoconjunctivitis sicca. CLAO J. 1985;11:243-250.
35. Yolton DP, Mende S, Harper A, et al. Association of dry eye signs and symptoms with tear lactoferrin concentration. J Am Optom Assoc. 1991;62:217-223.
36. Whitcher JP. Clinical diagnosis of the dry eye. Int Ophthalmol Clin. 1987;27:7-24.
37. Ren H, Wilson G. Apoptosis in the corneal epithelium. Invest Ophthalmol Vis Sci. 1996;37:1017-1025.
38. Yamamoto K, Ladage PM, Ren DH, et al. Effect of eyelid closure and overnight contact lens wear on viability of surface epithelial cells in rabbit cornea. Cornea. 2002;21:85-90.
39. Li L, Ren DH, Ladage PM, et al. Annexin V binding to rabbit corneal epithelial cells following overnight contact lens wear or eyelid closure. CLAO J. 2002;28:48-54.
40. Doughty MJ. Analyses of areas and shapes of cells on the corneal surface of the albino rabbit by scanning electron microscopy. Curr Eye Res. 1990;9:295-306.
41. Airman SA, Randers L, Rao G. Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations. Biotechnol Prog. 1993;9:671-674.
42. Means TL, Geroski DH, Hadley A, et al. Viability of human corneal endothelium following Optisol-GS storage. Arch Ophthalmol. 1995;113:805-809.
43. Patterson MK Jr. Measurement of growth and viability of cells in culture. Methods Enzymol. 1979;58:141-152.
44. Lemp MA, Mathers WD. Corneal epithelial cell movement in humans. Eye. 1989;3:438-445.
45. Choy EP, Cho P, Benzie IF, et al. A novel porcine dry eye model system (pDEM) with simulated lacrimation/blinking system: Preliminary findings on system variability and effect of corneal drying. Curr Eye Res. 2004;28:319-325.