Expression studies of GUP1 and GUP2, genes involved in glycerol active transport in Saccharomyces cerevisiae, using semi-quantitative RT-PCR

Current Genetics

Volumn 46, Issue 3, 2004, Pages 140-146

  Oliveira, Rui ^a   Lucas, Cândida ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Expression study; Glycerol transport; GUP1; GUP2; Saccharomyces cerevisiae; Semi quantitative reverse transcriptase polymerase chain reaction

Indexed keywords

ACETIC ACID; GLUCOSE; GLYCEROL; MEMBRANE PROTEIN; MUTANT PROTEIN; PROTEIN GUP1; PROTEIN GUP2; SODIUM CHLORIDE; TREHALOSE; UNCLASSIFIED DRUG;

ACTIVE TRANSPORT; ARTICLE; BIOACCUMULATION; CONTROLLED STUDY; CORRELATION FUNCTION; FUNGAL STRAIN; FUNGUS GROWTH; GENE ACTIVITY; GENE EXPRESSION; GENE REPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SALT STRESS;

ACETATES; BIOLOGICAL TRANSPORT, ACTIVE; CARRIER PROTEINS; DNA PRIMERS; GENE EXPRESSION REGULATION, FUNGAL; GLYCEROL; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MUTATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TREHALOSE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 4744353297     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-004-0519-3     Document Type: Article

References (33)

1. Aelst L van, Hohmann S, Zimmermann F, Jans A, Thevelein J (1991) A yeast homologue of the bovine lens fibre MIP gene family complements the growth defect of a Saccharomyces cerevisiae mutant on fermentable sugars but not its defect in glucose-induced RAS-mediated cAMP signalling. EMBO J 10:2095-2104
2. Albertyn J, Hohmann S, Thevelein J, Prior B (1994) GPD1, which encodes glycerol-3-phosphate dehydrogenase, is essential for growth under osmotic stress in Saccharomyces cerevisiae, and its expression is regulated by high-osmolarity glycerol response pathway. Mol Cell Biol 14:4135-4144
3. +-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J 16:2179-2187
4. Blomberg A (2000) Metabolic surprises in Saccharomyces cerevisiae during adaptation to saline conditions: questions, some answers and a model. FEMS Microbiol Lett 182:1-8
5. +) in acquired osmotolerance of Saccharomyces cerevisiae. J Bacteriol 171:1087-1092
6. Brown AD (1978) Compatible solute and extreme water stress in eukaryotic micro-organisms. Adv Microb Physiol 17:181-242
7. Causton HC, Ren B, Koh SS, Harbison CT, Kanin E, Jennings EG, Lee TI, True HL, Lander ES, Young RA (2001) Remodelling of yeast genome expression in response to environmental changes. Mol Biol Cell 12:323-337
8. Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, Botstein D, Brown PO (2000) Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell 11:4241-4257
9. Holst B, Lunde C, Lages F, Oliveira R, Lucas C, Kielland-Brandt MC (2000) GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae. Mol Microbiol 37:108-124
10. + symport in the halotolerant yeast Pichia sorbitophila. Yeast 11: 111-119
11. Lages F, Lucas C (1997) Physiological characterisation of mediated glycerol uptake in Saccharomyces cerevisiae. Biochim Biophys Acta 1322:8-18
12. Lages F, Silva-Graça M, Lucas C (1999) Active glycerol uptake is a mechanism underlying halotolerance in yeasts: a study of 42 species. Microbiology 145:2577-2585
13. Mager WH, Varela JCS (1993) Osmostress response of the yeast Saccharomyces. Mol Microbiol 10:253-258
14. May JW, Sloan J (1981) Glycerol utilization by Schizosaccharomyces pombe: dehydrogenation as the initial step. J Gen Microbiol 123:183-185
15. May JW, Marshall JH, Sloan J (1982) Glycerol utilization by Schizosaccharomyces pombe: phosphorylation of dihydroxyacetone by a specific kinase as the second step. J Gen Microbiol 128:1763-1766
16. Molin M, Norbeck J, Blomberg A (2003) Dihydroxyacetone kinases in Saccharomyces cerevisiae are involved in detoxification of dihydroxyacetone. J Biol Chem 278:1415-1423
17. Navarro-Aviño JP, Prasad R, Miralles VJ, Benito RM, Serrano R (1999) A proposal for nomenclature of aldehyde dehydrogenases in Saccharomyces cerevisiae and characterization of the stress-inducible ALD2 and ALD3 genes. Yeast 15:829-842
18. Norbeck J, Blomberg A (1997) Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via dihydroxiacetone pathway. J Biol Chem 272:5544-5554
19. Norbeck J, Blomberg A (2000) The level of cAMP-dependent protein kinase A activity strongly affects osmotolerance and osmo-instigated gene expression changes in Saccharomyces cerevisiae. Yeast 16:121-137
20. +/glycerol symport activity. FEMS Microbiol Lett 142:147-153
21. Oliveira R, Lages F, Silva-Graça M, Lucas C (2003) Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions. Biochim Biophys Acta 1631:57-71
22. Påhlman A-K, Granat K, Ansell R, Hohmann S, Adler L (2001) The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stress. J Biol Chem 276:3555-3563
23. Posas F, Chambers JR, Heyman JA, Hoeffler JP, Nadal E de, Arino J (2000) The trancriptional response of yeast to saline stress. J Biol Chem 275:17249-17255
24. Rep M, Krantz M, Thevelein JM, Hohmann S (2000) The transcriptional response of Saccharomyces cerevisiae tp osmotic shock. J Biol Chem 275:8290-8300
25. Robinson KS, Lai K, Cannon TA, McGraw P (1996) Inositol transport in Saccharomyces cerevisiae is regulated by transcriptional and degradative endocytic mechanisms during the growth cycle that are distinct from inositol-induced regulation. Mol Biol Cell 7:81-89
26. Rønnow B, Kielland-Brandt M (1993) GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae. Yeast 9:1121-1130
27. Séron K, Blondel M-O, Haguenauer-Tsapis R, Volland C (1999) Uracil-induced down-regulation of the yeast uracil permease. J Bacteriol 181:1793-1800
28. Silva-Graça M, Neves L, Lucas C (2003) Physiological studies of long-term adaptation to salt stress in the extremely halotolerant yeast Candida versatilis CBS 4019 (syn. C. halophila). Yeast FEMS Res 3:1-14
29. Tamás MJM, Luyten K, Sutherland CF, Hernadez A, Albertyn J, Valadi H, Li H, Prior BA, Killian S, Ramos J, Gustafsson L, Thevelein JM, Hohmann S (1999) Fps1p controls the accumulation and the release of the compatible solute glycerol in yeast osmoregulation. Mol Microbiol 31:1087-1104
30. Tamás MJ, Rep M, Thevelein JM, Hohmann S (2000) Stimulation of the yeast high osmolarity glycerol (HOG) pathway: evidence for a signal generated by a change in turgor rather than by water stress. FEBS Lett 472:159-165
31. Thomas BJ, Rothstein RJ (1989) Elevated recombination rates in transcriptionally active DNA. Cell 56:619-630
32. Yale J, Bohnert HJ (2001) Transcript expression in Saccharomyces cerevisiae at high salinity. J Biol Chem 276:15996-16007
33. Zyl PJ van, Kilian SG, Prior BA (1991) The role of an active transport mechanism in glycerol accumulation during osmo-regulation by Zygosaccharomyces rouxii. Appl Microbiol Biotechnol 34:231-235