Kinetic response of a Drosophila melanogaster cell line to different medium formulations and culture conditions

Cytotechnology

Volumn 57, Issue 1, 2008, Pages 23-35

  Bovo, R ^a   Galesi, A L L ^b   Jorge, S A C ^a   Piccoli, R A M ^c   Moraes, A M ^b   Pereira, C A ^a   Augusto, E F P ^c  

^a INSTITUTO BUTANTAN   (Brazil)

^b UNIVERSITY OF CAMPINAS   (Brazil)

^c Instituto de Pesquisas Tecnologicas   (Brazil)

Author keywords

Alanine; Glucose; Glutamine; Kinetic parameters; Lactate; Metabolism; S2 cells; SF900 II ; TC 100

Indexed keywords

AMINO ACID; GLUCOSE; GLUTAMINE; LACTIC ACID;

ANIMAL CELL; ARTICLE; CELL ACTIVITY; CELL CULTURE; CELL GROWTH; CELL LINE; CELL METABOLISM; CONTROLLED STUDY; CULTURE MEDIUM; DROSOPHILA MELANOGASTER; GLUCOSE TRANSPORT; GROWTH RATE; INSECT CELL CULTURE; KINETICS; NONHUMAN;

DROSOPHILA MELANOGASTER;

EID: 47349124508     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-008-9146-z     Document Type: Article

References (33)

1. Bachmann AS, Corpuz G, Hareld WP et al (2004) A simple method for the rapid purification of copia virus-like particles from Drosophila Schneider 2 cells. J Virol Methods 115:159-165
2. Banks DJ, Hua G, Adang M (2003) Cloning of a Heliothis virescens 110 kda aminopeptidase N and expression in Drosophila S2 cells. Insect Biochem Mol Biol 33:499-508
3. Bhatia R, Jesionowski G, Ferrance J et al (1997) Insect cell physiology. Cytotechnology 24:1-9
4. Deml L, Wolf H, Wagner R (1999) High level expression of hepatitis B virus surface antigen in stably transfected Drosophila Schneider-2 cells. J Virol Methods 79:191-203
5. Doverskog M, Ljunggren J, Öhman L et al (1997) Physiology of cultured animal cells. J Biotechnol 59:103-115
6. Drews M, Paalme T, Vilu R (1995) The growth and nutrient utilization of insect cell line Spodoptera frugiperda Sf9 in batch and continuous culture. J Biotechnol 40:187-198
7. 15N NMR. J Biotechnol 78:23-37
8. Ferrance JP, Goel A, Ataai MM (1993) Utilization of glucose and amino acids in insect cell cultures: quantifying the metabolic flows within the primary pathways and medium development. Biotechnol Bioeng 42:697-707
9. Freshney RI (2005) Culture of animal cells: a manual of basic technique, 5th edn. Wiley-Liss, New York, 642 p
10. Galesi ALL, Pereira CA, Moraes AM (2007) Culture of transgenic Drosophila melanogaster Schneider S2 cells in serum free media based on TC100 basal media. Biotechnol J 2(11):1399-1407
11. ALL Galesi MA Aguiar RM Astray 2008 Growth of recombinant Drosophila melanogaster Schneider 2 cells producing rabies virus glycoprotein in bioreactor employing serum-free medium Cytotechnology Galesi ALL, Aguiar MA, Astray RM et al (2008) Growth of recombinant Drosophila melanogaster Schneider 2 cells producing rabies virus glycoprotein in bioreactor employing serum-free medium. Cytotechnology. doi: 10.1007/s10616-008-9139-y
12. Gibson KR, Vanek PG, Kaloss WD et al (1993) Expression of dopamine-beta-hydroxylase in Drosophila Schneider 2 cells-Evidence for a mechanism of membrane-binding other than uncleaved signal peptide. J Biol Chem 268:9490-9495
13. Ikonomou L, Schneider YJ, Agathos SN (2003) Insect cell culture for industrial production of recombinant proteins. Appl Microbiol Biotechnol 62:1-20
14. Jeon HK, Chang KH, Kim KI et al (2003) Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells. Biotechnol Lett 25:185-189
15. Kirkpatrick RB, Ganguly S, Angelichio M et al (1995) Heavy chain dimers as well as complete antibodies are efficiently formed and secreted from Drosophila via a BiP-mediated pathway. J Biol Chem 270(34):19800-19805
16. Lee JM, Park JH, Park JO et al (2000) Expression of recombinant erythropoietin in stably transformed Drosophila melanogaster S2 cells. In Vitro Cell Dev Biol Anim 36:348-350
17. McCarroll L, King LA (1997) Stable insect cell cultures for recombinant protein production. Curr Opin Biotechnol 8:590-594
18. Mendonça RZ, Palomares LA, Ramírez OT (1999) An insight into insect cell metabolism through selective nutrient manipulation. J Biotechnol 72:61-75
19. Millar NS, Baylis HA, Reaper C et al (1995) Functional expression of a cloned Drosophila muscarinic acetylcholine receptor in a stable Drosophila cell line. J Exp Biol 198:1843-1850
20. Öhman L, Ljunggren J, Häggström L (1995) Induction of a metabolic switch in insect cells by substrate-limited fed-batch. Appl Microbiol Biotechnol 43:1006-1013
21. Park JH, Lee JM, Chung IS (1999) Production of recombinant endostatin from stably transformed Drosophila melanogaster S2 cells. Biotechnol Lett 21:729-733
22. Perret BG, Wagner R, Lecat S et al (2003) Expression of EGFP-amino-tagged human mu opioid receptor in Drosophila Schneider 2 cells: a potential expression system for large-scale production of g-protein coupled receptors. Protein Expr Purif 31:123-132
23. Rhiel M, Mitchell-Logean CM, Murhammer DW (1997) Comparison of Trichoplusia ni BTI-Tn-5B1-4 (High Five TM) and Spodoptera frugiperda Sf-9 insect cell line metabolism in suspension cultures. Biotechnol Bioeng 55:909-920
24. Santos MG, Jorge SAC, Brillet K et al (2007) Improving heterologous protein expression in transfected Drosophila S2 cells as assessed by EGFP expression. Cytotechnology 54:15-24
25. Sondergaard L (1996) Drosophila cells can be grown to high cell densities in bioreactor. Biotechnol Tech 10:161-166
26. Sugiura T, Amann E (1996) Properties of two insect cell lines useful for the baculovirus expression system in serum-free culture. Biotechnol Bioeng 51:494-499
27. Swiech K, Silva CL, Arantes MK et al (2007) Characterization of growth and metabolism of Drosophila melanogaster cells transfected with the rabies virus glycoprotein gene. Biotechnol Appl Biochem 49:41-49
28. K Swiech N Rossi BG Silva 2008 Bioreactor culture of recombinant Drosophila melanogaster S2 cells: characterization of metabolic features related to cell growth and production of the rabies virus glycoprotein Cytotechnology Swiech K, Rossi N, Silva BG et al (2008) Bioreactor culture of recombinant Drosophila melanogaster S2 cells: characterization of metabolic features related to cell growth and production of the rabies virus glycoprotein. Cytotechnology. doi: 10.1007/s10616-008-9130-7
29. Torfs H, Shariatmadari R, Guerrero F et al (2000) Characterization of a receptor for insect tachykinin-like peptide agonists by functional expression in a stable Drosophila Schneider 2 cell line. J Neurochem 74:2182-2189
30. Tota MR, Xu L, Sirotina A et al (1995) Interaction of [fluorescein-Trp25] glucagon with the human glucagon receptor expressed in Drosophila Schneider-2 cells. J Biol Chem 270:26466-26472
31. Valle MA, Kester MB, Burns AL et al (2001) Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor. Cytotechnology 35:127-135
32. Vanden Broeck J, Vulsteke V, Huybrechts R et al (1995) Characterization of a cloned locust tyramine receptor cDNA by functional expression in permanently transformed Drosophila S2 cells. J Neurochem 64:2387-2395
33. Yokomizo AY, Jorge SAC, Astray RM et al (2007) Rabies virus glycoprotein expression in Drosophila S2 cells. I. Functional recombinant protein in stable co-transfected cell line. Biotechnol J 2:1-8