High genetic variability in non-aflatoxigenic A. flavus strains by using Quadruplex PCR-based assay

International Journal of Food Microbiology

Volumn 125, Issue 3, 2008, Pages 341-343

  Criseo, Giuseppe ^a   Racco, Cosimo ^a   Romeo, Orazio ^a  

^a UNIVERSITY OF MESSINA   (Italy)

Author keywords

Aflatoxin; Aflatoxin non producing A. flavus strains; Aspergillus flavus; nor 1, omt A, ver 1 and aflR genes

Indexed keywords

DNA FRAGMENT; FUNGAL DNA;

ARTICLE; ASPERGILLUS FLAVUS; FOOD; FUNGAL STRAIN; FUNGUS ISOLATION; GENE FREQUENCY; GENETIC VARIABILITY; NONHUMAN; POLYMERASE CHAIN REACTION; STRUCTURAL GENE;

AFLATOXINS; ASPERGILLUS FLAVUS; DNA FRAGMENTATION; DNA, FUNGAL; FOOD MICROBIOLOGY; POLYMERASE CHAIN REACTION; VARIATION (GENETICS);

ASPERGILLUS FLAVUS;

EID: 47349112400     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2008.04.020     Document Type: Article

References (16)

1. Bennett J.W., and Klich M. Mycotoxins. Clinical Microbiology Reviews 16 (2003) 497-516
2. Bhatnagar D., Cary J.W., Ehrlich K., Yu J., and Cleveland T.E. Understanding the genetics of regulation of aflatoxin production and Aspergillus flavus development. Mycopathologia 162 (2006) 155-166
3. Carbone I., Ramirez-Prado J.H., Jakobek J.L., and Horn B.W. Gene duplication, modularity and adaptation in the evolution of the aflatoxin gene cluster. BMC Evolutionary Biology 7 (2007) 111
4. Cary J.W., and Ehrlich K.C. Aflatoxigenicity in Aspergillus: molecular genetics, phylogenetic relationships and evolutionary implications. Mycopathologia 162 (2006) 167-177
5. Chang P.K., Horn B.W., and Dorner J.W. Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in non-aflatoxigenic Aspergillus flavus isolates. Fungal Genetics and Biology 42 (2005) 914-923
6. Criseo G., Bagnara A., and Bisignano G. Differentiation of aflatoxin-producing and non-producing strains of Aspergillus flavus group. Letters in Applied Microbiology 33 (2001) 291-295
7. Degola F., Berni E., Dall'Asta C., Spotti E., Marchelli R., Ferrero I., and Restivo F.M. A multiplex RT-PCR approach to detect aflatoxigenic strains of Aspergillus flavus. Journal of Applied Microbiology 103 (2007) 409-417
8. Färber P., Geisen R., and Holzapfel W.H. Detection of aflatoxigenic fungi in figs by a PCR reaction. International Journal of Food Microbiology 36 (1997) 215-220
9. Geisen R. Multiplex polymerase chain reaction for the detection of potential aflatoxin and sterigmatocystin producing fungi. Systematic and Applied Microbiology 19 (1996) 388-392
10. Manonmani H.K., Anand S., Chandrashekar A., and Rati E.R. Detection of aflatoxigenic fungi in selected food commodities by PCR. Process Biochemistry 40 (2005) 2859-2864
11. Mayer Z., Bagnara A., Färber P., and Geisen R. Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods. International Journal of Food Microbiology 82 (2003) 143-151
12. Scherm B., Palomba M., Serra D., Marcello A., and Migheli Q. Detection of transcripts of the aflatoxin genes aflD, aflO, and aflP by reverse transcription-polymerase chain reaction allows differentiation of aflatoxin-producing and non-producing isolates of Aspergillus flavus and Aspergillus parasiticus. International Journal of Food Microbiology 98 (2005) 201-210
13. Shapira R., Paster N., Eyal O., Menasherov M., Mett A., and Salomon R. Detection of aflatoxigenic molds in grains by PCR. Applied and Environmental Microbiology 62 (1996) 3270-3273
14. Somashekar D., Rati E.R., and Chandrashekar A. PCR-restriction fragment length analysis of aflR gene for differentiation and detection of Aspergillus flavus and Aspergillus parasiticus in maize. International Journal of Food Microbiology 93 (2005) 101-107
15. Yelton M.M., Hamer J.E., and Timberlake W.E. Transformation of Aspergillus nidulans by using a trpC plasmid. Proceedings of the National Academy of Science USA 81 (1984) 1470-1474
16. Zachova I., Vytrasova J., Pejchalova M., Cervenka L., and Tavcar-Kalcher G. Detection of aflatoxigenic fungi in feeds using the PCR method. Folia Microbiologica 48 (2003) 817-821