Elastase inhibition assay with peptide substrates - An example for the limited comparability of in vitro results

Planta Medica

Volumn 74, Issue 8, 2008, Pages 852-858

  Groth, Inken ^a   Alban, Susanne ^a  

^a UNIVERSITY OF KIEL   (Germany)

Author keywords

Chromogenic assay; Elastase; Enzyme inhibition assay; PMNE; Sulfated polysaccarides; Synthetic substrate; Ursolic acid

Indexed keywords

COUMARIN DERIVATIVE; ELASTASE; ELASTASE INHIBITOR; GLUCAN; HEPARIN; L 1270; L 1335; METHOXYSUCCINYLALANYLALANYLPROLYLVALYL 4 NITROANILIDE; METHOXYSUCCINYLALANYLALANYLPROLYLVALYL 7 AMIDO 4 METHYLCOUMARIN; PEPTIDE DERIVATIVE; POLYSACCHARIDE; PS 3; PYROGLUTAMYLPROLYLVALYL 4 NITROANILIDE; S 2484; URSOLIC ACID;

ARTICLE; COMPARATIVE STUDY; DELESSERIA SANGUINEA; ENZYME INHIBITION; ENZYME SUBSTRATE; IC 50; IN VITRO STUDY; NONHUMAN; RED ALGA;

ENZYME INHIBITORS; HUMANS; INHIBITORY CONCENTRATION 50; LEUKOCYTE ELASTASE; PEPTIDES; REFERENCE STANDARDS; SUBSTRATE SPECIFICITY;

DELESSERIA SANGUINEA; RHODOPHYTA;

EID: 47249127291     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2008-1074549     Document Type: Article

References (26)

1. Borregaard N, Cowland JB. Granules of the human neutrophilic poly-morphonuclear leukocyte. Blood 1997; 89: 3503-21
2. Bank U, Ansorge S. More than destructive: neutrophil-derived serine proteases in cytokine bioactivity control. J Leukoc Biol 2001; 69: 197-206
3. Janoff A. Elastase in tissue injury. Annu Rev Med 1985; 36: 207-16
4. Bernstein PR, Edwards PD, Williams JC. Inhibitors of human leukocyte elastase. Prog Med Chem 1994; 31: 59-120
5. Döng G. The role of neutrophil elastase in chronic inflammation. Am J Resp Crit Care 1994; 150: S114-7
6. ow O, Wiese F, Streit V, Kalm C, Christophers Eional elastase activity in psoriasis, contact dermatitis, and atopic dermatitis. J Invest Dermatol 1992; 99: 306-9
7. le B, Hrenn A, Merfort Iural compounds as inhibitors of human neutrophil elastase. Planta Med 2007; 73: 401-20
8. yashi Htrophil elastase inhibitors as treatment for COPD. Expert Opin Inv Drug 2002; 11: 965-80
9. Melzig ME, loser B, Ciesielski S. Inhibition of neutrophil elastase activity by phenolic compounds from plants. Pharmazie 2001; 56: 967-70
10. Skiles JW, Miao C, Sorcek R, Jacober S, Mui PW, Chow G et al. Inhibition of human leukocyte elastase by N-substituted peptides containing alpha.alpha-difluorostatone residues at P1. J Med Chem 1992; 35: 4795-808
11. Steinmeyer J, Kalbhen DA. Pharmacological influence on polymorphonuclear granulocytes elastase under various test conditions. Arznei-mittelforschung 1990; 40: 196-200
12. Lestienne P, Bieth JG. Activation of human leukocyte elastase activity by excess substrate, hydrophobic solvents, and ionic strength. J Biol Chem 1980; 255: 9289-94
13. Sartor L, Pezzato E, Dell'Aica I, Caniato R, Biggin S, Garbisa S. Inhibition of matrix-proteases by polyphenols: chemical insights for anti-inflammatory and anti-invasion drug design. Biochem Pharmacol 2002; 64: 229-37
14. Becker M, Franz G, Atban S. Inhibition of PMN-elastase activity by semisynthetic glucan sulfates. Thromb Haemost 2003; 89: 915 - 25
15. Yvin J, Alban S, Franz G. Anti-inflammatory and healing medicine based on laminarin sulfate. PCT Int Appl Patent No WO 2002 036 132; May 2002
16. Grünewald N, Alban S. Optimized isolation and structural characterization of biologically active sulfated polysaccharides from the red alga Delesseria sanguinea (Hudson) Lamouroux. Planta Med 2007; 73: 932
17. Macdonald SJF, Dowle MD, Harrison LA, Shah P, Johnson MR, Inglis GGA et al. The discovery of a potent, intracellular, orally bioavailable, long duration inhibitor of human neutrophil elastase - GW311616A a development candidate. Bioorg Med Chem Lett 2001; 11: 895 - 8
18. Pannell R, Johnson D, Travis J. Isolation and properties of human plasma α-1-proteinase inhibitor. Biochemistry 1974; 13: 5439-45
19. Redini F, TixierJM, Petitou M, Choay J, Robert, Homebeck W. Inhibition of leucocyte elastase by heparin and its derivatives. Biochem J 1988; 252: 515-9
20. Ying QL, Rinehart AR, Simon SR, Cheronis JC. Inhibition of human leucocyte elastase by ursolic acid. Evidence for a binding site for pentacyclic triterpenes. Biochem J 1991; 277: 521 - 6
21. Padrines M, Bieth JG. Elastin decreases the efficiency of neutrophil elastase inhibitors. Am J Respir Cell Mol Biol 1991; 4: 187 - 93
22. Stein RL. Catalysis by human leukocyte elastase: Substrate structural dependence of rate-limiting protolytic catalysis and operation of the charge relay system. J Am Chem Soc 1983; 105: 5111-6
23. Stein RL. Catalysis by human leukocyte elastase. 4. Role of secondary-subsite interactions. J Am Chem Soc 1985; 107: 5767-75
24. Shin JS, Yu MH. Kinetic dissection of alphal-antitrypsin inhibition mechanism.J Biol Chem 2002; 277: 11629-35
25. Huntington JA, Read RJ, Carrell RW. Structure of a serpin-protease complex shows inhibition by deformation. Nature 2000; 407: 923 - 6
26. Spencer JL, Stone PJ, Nugent MA. New insights into the inhibition of human neutrophil elastase by heparin. Biochemistry 2006; 45: 9104-20