X-ray crystallographic studies of substrate binding to aristolochene synthase suggest a metal ion binding sequence for catalysis

Journal of Biological Chemistry

Volumn 283, Issue 22, 2008, Pages 15431-15439

  Shishova, Ekaterina Y ^a   Yu, Fanglei ^b   Miller, David J ^b   Faraldos, Juan A ^c   Zhao, Yuxin ^c   Coates, Robert M ^c   Allemann, Rudolf K ^b   Cane, David E ^d   Christianson, David W ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^d BROWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING SITES; CATALYSIS; CRYSTAL STRUCTURE; ENZYMES; FLUORESCENCE; HYDROCARBONS; IONS; METAL IONS; MONOMERS; PUMPING PLANTS;

ACTIVE SITES; ASPERGILLUS TERREUS; CONFORMATIONAL CHANGES; CONFORMATIONAL TRANSITIONS; CRYSTALLOGRAPHIC STUDIES; DIPHOSPHATE; FARNESYL DIPHOSPHATE; INITIAL STAGES; METAL ION BINDINGS; OPEN CONFORMATIONS; PRODUCT COMPLEXES; REACTION CATALYZED; RESOLUTION STRUCTURES; SESQUITERPENE; STRUCTURAL CHANGES; SUBSTRATE BINDINGS; SYNTHASE; TETRAMER;

CONFORMATIONS;

12, 13 DIFLUOROFARNESYL DIPHOSPHATE; 2 FLUOROFARNESYL DIPHOSPHATE; ARISTOLOCHENE; ARISTOLOCHENE SYNTHETASE; FARNESYL DIPHOSPHATE; HYDROCARBON; MAGNESIUM ION; MONOMER; PYROPHOSPHATE; SYNTHETASE; TETRAMER; UNCLASSIFIED DRUG; 12,13 DIFLUOROFARNESYL DIPHOSPHATE; 12,13-DIFLUOROFARNESYL DIPHOSPHATE; ARISTOLOCHENE SYNTHASE; FUNGAL PROTEIN; ISOMERASE; ISOPRENOID PHOSPHATE; MAGNESIUM; SESQUITERPENE;

ARTICLE; ASPERGILLUS TERREUS; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME MECHANISM; METAL BINDING; PRIORITY JOURNAL; PROTEIN BINDING; X RAY CRYSTALLOGRAPHY; ASPERGILLUS; CHEMISTRY; ENZYMOLOGY; METABOLISM; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE;

ASPERGILLUS TERREUS;

ASPERGILLUS; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; FUNGAL PROTEINS; ISOMERASES; MAGNESIUM; POLYISOPRENYL PHOSPHATES; PROTEIN STRUCTURE, TERTIARY; SESQUITERPENES;

EID: 47249119324     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M800659200     Document Type: Article

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