Functional mapping of the Candida albicans Efg1 regulator

Eukaryotic Cell

Volumn 7, Issue 5, 2008, Pages 881-893

  Noffz, Christine S ^a   Liedschulte, Vanessa ^a   Lengeler, Klaus ^a   Ernst, Joachim F ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CZF1 PROTEIN, CANDIDA ALBICANS; DNA BINDING PROTEIN; EFG1 PROTEIN, CANDIDA ALBICANS; FUNGAL PROTEIN; HELIX LOOP HELIX PROTEIN; REPRESSOR PROTEIN; TRANSCRIPTION FACTOR;

ALLELE; ARTICLE; CANDIDA ALBICANS; CHEMISTRY; CONSENSUS SEQUENCE; CYTOLOGY; DNA RESPONSIVE ELEMENT; DRUG ANTAGONISM; GEL MOBILITY SHIFT ASSAY; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHENOTYPE; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; REGULATOR GENE; TWO HYBRID SYSTEM;

ALLELES; CANDIDA ALBICANS; CONSENSUS SEQUENCE; DNA-BINDING PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, REGULATOR; HELIX-LOOP-HELIX MOTIFS; PHENOTYPE; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; REPRESSOR PROTEINS; RESPONSE ELEMENTS; SEQUENCE DELETION; TRANSCRIPTION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

CANDIDA ALBICANS;

EID: 47049118329     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00033-08     Document Type: Article

References (26)

1. Arnaud, M. B., M. C. Costanzo, M. S. Skrzypek, G. Binkley, C. Lane, S. R. Miyasato, and G. Sherlock. 2005. The Candida Genome Database (CGD), a community resource for Candida albicans gene and protein information. Nucleic Acids Res. 33:D358-D363.
2. Bockmühl, D. P., and J. F. Ernst. 2001. A potential phosphorylation site for an A-kinase in the Efg1 regulator protein contributes to hyphal morphogenesis of Candida albicans. Genetics 157:1523-1530.
3. Bockmühl, D. P., S. Krishnamurthy, M. Gerads, A. Sonneborn, and J. F. Ernst. 2001. Distinct and redundant roles of the two protein kinase A isoforms Tpk1p and Tpk2p in morphogenesis and growth of Candida albicans. Mol. Microbiol. 42:1243-1257.
4. Cao, F., S. Lane, P. P. Raniga, Y. Lu, Z. Zhou, K. Ramon, J. Chen, and H. Liu. 2006. The Flo8 transcription factor is essential for hyphal development and virulence in Candida albicans. Mol. Biol. Cell 17:295-307.
5. Doedt, T., S. Krishnamurthy, D. P. Bockmühl, B. Tebarth, C. Stempel, C. L. Russell, A. J. P. Brown, and J. F. Ernst. 2004. APSES proteins regulate morphogenesis and metabolism in Candida albicans. Mol. Biol. Cell 15:3167-3180.
6. Dutton, J. R., S. Johns, and B. L. Miller. 1997. StuA is a sequence-specific transcription factor that regulates developmental complexity in Aspergillus nidulans. EMBO J. 16:5710-5721.
7. Fonzi, W. A., and M. Y. Irwin. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717-728.
8. Gimeno, C. J., and G. R. Fink. 1994. Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol. Biol. Cell 14:2100-2112.
9. Giusani, A. D., M. Vinces, and C. A. Kumamoto. 2002. Invasive filamentous growth of Candida albicans is promoted by Czf1p-dependent relief of Efg1p-mediated repression. Genetics 160:1749-1753.
10. Golemis, E. A. and R. Brent. 1997. Searching for Interacting proteins with the two-hybrid system. In P. Bartel and S. Fields (ed.), The yeast two-hybrid system. Oxford University Press, New York, NY.
11. James, P., J. Haladay, and E. A. Craig. 1996. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144:1425-1436.
12. Lee, K. L., H. R. Buckley, and C. C. Campbell. 1975. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148-153.
13. Leng, P., P. R. Lee, H. Wu, and A. J. Brown. 2001. Efg1, a morphogenetic regulator in Candida albicans, is a sequence-specific DNA binding protein. J. Bacteriol. 183:4090-4093.
14. Leuker, C. E., A. Sonneborn, S. Delbrück, and J. F. Ernst. 1997. Sequence and promoter regulation of the PCK1 gene encoding phosphoenolpyruvate carboxykinase of the fungal pathogen Candida albicans. Gene 192:235-240.
15. Linding, R., R. B. Russell, V. Neduva, and T. J. Gibson. 2003. GlobPlot: exploring protein sequences of globularity and disorder. Nucleic Acids Res. 13:3701-3708.
16. Lo, H. J., J. R. Köhler, B. DiDomenico, D. Loebenberg, A. Cacciapuoti, and G. R. Fink. 1997. Nonfilamentous C. albicans mutants are avirulent. Cell 90:939-949.
17. Russell, C. L., and A. J. P. Brown. 2005. Expression of one-hybrid fusions with Staphylococcus aureus lexA in Candida albicans confirms that Nrg1 is a transcriptional repressor and that Gcn4 is a transcriptional activator. Fungal Genet. Biol. 8:676-683.
18. Setiadi, E. R., T. Doedt, F. Cottier, C. Noffz, and J. F. Ernst. 2006. Transcriptional response of Candida albicans to hypoxia: linkage of oxygen-sensing and Efg1p-regulatory networks. J. Mol. Biol. 361:399-411.
19. Sherman, F., G. R. Fink, and J. B. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Sonneborn, A., D. P. Bockmühl, and J. F. Ernst. 1999. Chlamydospore formation in Candida albicans requires the Efg1p morphogenetic regulator. Infect. Immun. 67:5514-5517.
21. Sonneborn, A., B. Tebarth, and J. F. Ernst. 1999. Control of white-opaque phenotypic switching in Candida albicans by the Efg1p morphogenetic regulator. Infect. Immun. 67:4655-4660.
22. Srikantha, T., L. K. Tasai, K. Daniels, and D. R. Soll. 2000. EFG1 null mutants of Candida albicans switch but cannot express the complete phenotype of white-phase budding cells. J. Bacteriol. 182:1580-1591.
23. Stoldt, V. R., A. Sonneborn, C. E. Leuker, and J. F. Ernst. 1997. Efg1p, an essential regulator of morphogenesis of the human fungal pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi. EMBO J. 16:1982-1991.
24. Tebarth, B., T. Doedt, S. Krishnamurthy, M. Weide, F. Monterola, A. Dominguez, and J. F. Ernst. 2003. Adaptation of the Efg1p morphogenetic pathway in Candida albicans by negative autoregulation and PKA-dependent repression of the EFG1 gene. J. Mol. Biol. 329:949-962.
25. Vinces, M. D., C. Haas, and C. A. Kumamoto. 2006. Expression of the Candida albicans morphogenesis regulator gene CZF1 and its regulation by Efg1p and Czf1p. Eukaryot. Cell 5:825-835.
26. Wilson, R. B., D. Davis, and A. P. Mitchell. 1999. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions. J. Bacteriol. 181:1868-1874.