Evaluation of the Litron In Vitro MicroFlow® Kit for the flow cytometric enumeration of micronuclei (MN) in mammalian cells

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 654, Issue 1, 2008, Pages 76-81

  Collins, Joanne E ^a   Ellis, Patricia C ^b   White, Angela T ^a   Booth, Antonia E G ^a   Moore, Claire E ^c   Burman, Mark ^a   Rees, Robert W ^a   Lynch, Anthony M ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

^b PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United Kingdom)

^c UNILEVER RESEARCH   (United Kingdom)

Author keywords

Cytotoxicity; Flow cytometry; Genotoxicity testing; Micronuclei

Indexed keywords

DEXAMETHASONE; DIMETHYLBENZ[A]ANTHRACENE; MESYLIC ACID METHYL ESTER; STAUROSPORINE; VINBLASTINE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CHROMATIN; CONCENTRATION RESPONSE; CULTURE MEDIUM; CYTOTOXICITY; DEVICE; EVALUATION; FLOW CYTOMETRY; GENOTOXICITY; INTERMETHOD COMPARISON; MAMMAL CELL; MICRONUCLEUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; TOXICITY TESTING;

ANIMALS; APOPTOSIS; CELL LINE, TUMOR; FLOW CYTOMETRY; MICE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MUTAGENS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

MAMMALIA;

EID: 46849089321     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2008.05.003     Document Type: Article

References (22)

1. Nusse M., and Kramer J. Flow cytometric analysis of micronuclei found in cells after irradiation. Cytometry 5 (1984) 20-25
2. Roman D., Locher F., Suter W., Cordier A., and Bobadilla M. Evaluation of a new procedure for the flow cytometric analysis of in-vitro, chemically induced micronuclei in V79 cells. Environ. Mol. Mutagen. 32 (1998) 387-396
3. ® Kit User Manual, Litron Laboratories.
4. +/- mouse lymphoma specific gene and chromosomal mutation assay a phase III report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutat. Res. 394 (1997) 177-303
5. Jordan M.A., Thrower D., and Wilson L. Mechanism of inhibition of cell proliferation by Vinca alkaloids. Cancer Res. 51 (1991) 2212-2222
6. Kruman I.I., Matylevich N.P., Beletsky I.P., Afanasyev V.N., and Umansky S.R. Apoptosis of murine BW 5147 thymoma cells induced by dexamethasone and γ-irradiation. J. Cell. Physiol. 148 (1991) 267-273
7. Bertrand R., Solary E., O'Connor P., Kohn K.W., and Pommer Y. Induction of a common pathway of apoptosis by staurosporine. Exp. Cell. Res. 211 (1994) 314-321
8. McGregor D.B., Edwards I., Riach C.G., Cattanach P., Martin R., Mitchell A., and Caspary W.J. Studies of an S9-based metabolic activation system used in the mouse lymphoma L5178Y cell mutation assay. Mutagenesis 3 (1988) 485-490
9. Moore C.E., Collins J.E., Hynes G.M., Harvey J.S., Lynch A.M., Rees R.W., and Dertinger S.D. Evaluation of the Litron XCell MN Kit™ for the flow cytometric enumeration of micronuclei in L5178Y mouse lymphoma cells treated with MMS. Poster Presented at the 35th Annual Meeting of the European Environmental Mutagen Society-Environment and Human Genetic Disease-Causes, Mechanisms and Effects. Kos, Greece (2005) 3-7
10. Collins J.E., Booth A.E.G., Moore C.E., Hynes G.M., Ellis P.C., Harvey J.S., Avlasevich S.L., Dertinger S.D., Rees R.W., and Lynch A.M. Further evaluation of the Litron XCell MN Kit™ for the flow cytometric enumeration of micronuclei in L5178Y mouse lymphoma cells treated with DMBA. Poster Presented at the British Toxicology Society and the United Kingdom Environmental Mutagen Society Joint Congress. University of Warwick, UK (2006) 19-22
11. Collins J.E., Ellis P.C., Booth A.E.G., Moore C.E., Harvey J.S., Avlasevich S.L., Dertinger S.D., Rees R.W., and Lynch A.M. Continued evaluation of the Litron XCell MN Kit™ for the flow cytometric enumeration of micronuclei with L5178Y mouse lymphoma cells. Treatment with dexamethasone and staurosporine. Poster Presented at the 36th Annual Meeting of the European Environmental Mutagen Society-From Genes to Molecular Epidemiology. Prague, Czech Republic (2006) 2-6
12. Avlasevich S.L., Bryce S.T., Cairns S.E., and Dertinger S.D. In Vitro micronucleus scoring by flow cytometry: differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliability. Environ. Mol. Mutagen. 47 (2006) 56-66
13. Countryman P.I., and Heddle J.A. The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutat. Res. 41 (1976) 321-332
14. Collins J.E., White A.T., Ellis P.C., Burman M., Harvey J.S., Avlasevich S.L., Dertinger S.D., Rees R.W., and Lynch A.M. Further evaluation of the Litron XCell MN Kit™ for the flow cytometric enumeration of micronuclei in L5178Y mouse lymphoma cell treatments over 24 hours. Poster Presented at the Genome Stability Network and the United Kingdom Environmental Mutagen Society Joint Congress. University of Cardiff, UK (2007) 1-4
15. BD Pharmingen™ Annexin V-FITC Apoptosis Detection Kit I Technical Data Sheet.
16. Lovell D.P., Anderson D., Albanese R., Amphlett G.E., Clare G., Ferguson R., et al. Statistical analysis of in vivo cytogenetic assays. In: Kirkland D.J. (Ed). Statistical Evaluation of Mutagenicity Test Data (UKEMS Sub-committee on Guidelines for Mutagenicity Testing. Report. Part III) (1989), Cambridge University Press 184-232
17. Earnshaw W.C. Nuclear changes in apoptosis. Curr. Opin. Cell Biol. 7 (1995) 337-343
18. Lazebnik Y.A., Cole S., Cooke C.A., Nelson W.G., and Earnshaw W.C. Nuclear events of apoptosis in vitro in cell-free mitotic extracts: a model system for analysis of the active phase of apoptosis. J. Cell Biol. 123 (1993) 7-22
19. Ihara T., Yamamoto T., Sugamata M., Okumura H., and Ueno Y. The process of ultrastructural changes from nuclei to apoptotic body. Virchows Arch. 433 (1998) 443-447
20. Jacobson M.D., Weil M., and Raff M.C. Constitutive expression of the machinery for programmed cell death. J. Cell Biol. 133 (1996) 1053-1059
21. Jacobson M.D., Burne J.F., and Raff M.C. Mechanisms of programmed cell death and Bcl-2 protection. Biochem. Soc. Trans. 22 (1994) 600-602
22. Singh H., Singh J.R., Dhillon V.S., Bali D., and Paul H. In vitro and in vivo genotoxicity evaluation of hormonal drugs. II Dexamethasone. Mutat. Res. 308 (1994) 89-97