Correlative fluorescence and electron microscopy of biocytin-filled neurons with a preservation of the postsynaptic ultrastructure

Journal of Neuroscience Methods

Volumn 117, Issue 1, 2002, Pages 81-85

  Morozov, Youri ^a   Khalilov, Ilgam ^a   Ben Ari, Yehezkel ^a   Represa, Alfonso ^a  

^a Turing Center for Living Systems CENTURI Aix Marseille University   (France)

Author keywords

ABC; Filopodia; Fluorophore; Growth cones; Rat hippocampus; Streptavidin; Synapses; Ultra small gold

Indexed keywords

ALDEHYDE; AVIDIN; BIOCYTIN; GOLD; OSMIUM TETRAOXIDE; RESIN; SILVER; STREPTAVIDIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DENSITY; CELL ORGANELLE; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CORRELATION ANALYSIS; DENDRITIC SPINE; ELECTRON MICROSCOPY; FILOPODIUM; FLUORESCENCE MICROSCOPY; HIPPOCAMPUS; NERVE CELL; NERVE FIBER; NONHUMAN; PATCH CLAMP; PERINATAL PERIOD; POSTSYNAPTIC MEMBRANE; PRIORITY JOURNAL; RAT; SYNAPSE; VALIDATION PROCESS;

ANIMALS; ANIMALS, NEWBORN; AXONS; DENDRITES; FIXATIVES; FLUORESCENT DYES; HIPPOCAMPUS; IMMUNOHISTOCHEMISTRY; INTERNEURONS; LYSINE; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE; NEUROANATOMY; NEURONS; ORGAN CULTURE TECHNIQUES; ORGANELLES; PYRAMIDAL CELLS; RATS; STREPTAVIDIN; SYNAPTIC MEMBRANES; TISSUE EMBEDDING; TISSUE FIXATION;

EID: 0037198390     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(02)00076-6     Document Type: Article

References (11)

1. Glutamatergic synapses on oligodendrocyte precursor cells in the hippocampus
2. Combining laser scanning confocal microscopy and electron microscopy to determine sites of synaptic contact between two identified neurons
3. Morphology of CA3 non-pyramidal cells in the developing rat hippocampus
4. Total number and ratio of excitatory and inhibitory synapses converging onto single interneurons of different types in the CA1 area of the rat hippocampus
5. Antisera to γ-aminobutyric acid. I. Production and characterization using a new model system
6. Early development of neuronal activity in the primate hippocampus in utero
7. Total number and distribution of inhibitory and excitatory synapses on hippocampal CA1 pyramidal cells
8. The apical shaft of CA1 pyramidal cells is under gabaergic interneuronal control
9. Antisera to γ-aminobutyric acid. III. Demonstration of GABA in Golgi-impregnated neurons and in conventional electron microscopic sections of cat striate cortex
10. Immunogold demonstration of GABA in synaptic terminals of intracellularly recorded, horseradish peroxidase-filled basket cells and clutch cells in the cat's visual cortex
11. The establishment of GABAergic and glutamatergic synapses on CA1 pyramidal neurons is sequential and correlates with the development of the apical dendrite