Expression and characterization of polyketide synthase module involved in the late step of cephabacin biosynthesis from Lysobacter lactamgenus

Journal of Microbiology and Biotechnology

Volumn 18, Issue 3, 2008, Pages 427-433

  Lee, Ji Seon ^a   Vladimirova, Miglena G ^a   Demirev, Atanas V ^a   Kim, Bo Geum ^a   Lim, Si Kyu ^b   Nam, Doo Hyun ^a  

^a Yeungnam University   (South Korea)

^b GenoTech Corporation   (South Korea)

Author keywords

ketoacyl synthase; Acyl carrier protein; Acyltransferase; Cephabacin; Polyketide synthase

Indexed keywords

ACETYLCYSTEAMINE; ACYL CARRIER PROTEIN; ACYLTRANSFERASE; ALANINE; ALANYLALANYLALANYLARGININE; ARGININE; BACTERIAL ENZYME; BETA KETOACYL SYNTHASE; BETA LACTAM ANTIBIOTIC; CEPHABACIN; CYSTEAMINE DERIVATIVE; HISTIDINE; HYBRID PROTEIN; MALONYL COENZYME A; NICKEL; PEPTIDE SYNTHASE; POLYKETIDE SYNTHASE; PROTEIN SFP; RECOMBINANT ENZYME; SYNTHETASE; TETRAPEPTIDE; 3 OXOACYL ACYL CARRIER PROTEIN SYNTHASE; BACTERIAL PROTEIN; CEPHABACINS; CEPHALOSPORIN DERIVATIVE;

ACETYLATION; ACYLATION; AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; DECARBOXYLATION; DRUG SYNTHESIS; ENZYME ANALYSIS; ENZYME STABILITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; GENETIC CODE; IN VITRO STUDY; LYSOBACTER; LYSOBACTER LACTAMGENUS; MOLECULAR CLONING; NONHUMAN; NONRIBOSOMAL PEPTIDE SYNTHESIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN TERTIARY STRUCTURE;

LYSOBACTER; LYSOBACTER LACTAMGENUS;

3-OXOACYL-(ACYL-CARRIER-PROTEIN) SYNTHASE; ACYL CARRIER PROTEIN; ACYLTRANSFERASES; BACTERIAL PROTEINS; CEPHALOSPORINS; GENE EXPRESSION; GENETIC VECTORS; LYSOBACTER; POLYKETIDE SYNTHASES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

EID: 46749103392     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72: 248-254.
2. Cheng, Y. Q., G. L. Tang, and B. Shen. 2003. Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis. Proc. Natl. Acad. Sci. USA 100: 3149-3154.
3. Demirev, A. V, C. H. Lee, B. P. Jaishy, D. H. Nam, and D. D. Y. Ryu. 2006. Substrate specificity of nonribosomal peptide synthetase modules responsible for the biosynthesis of die oligopeptide moiety of cephabacin in Lysobacter lactamgenus. FEMS Microbiol. Lett. 255: 121-128.
4. Jaishy, B. P., S. K. Lim, I. D, Yoo, J. C. Yoo, J. K. Sohng, and D. H. Nam. 2006. Cloning and characterization of a gene cluster for the production of polyketide macrolide dihydrochalcomycin in Streptomyces sp. KCTC 0041BP. J. Microbiol. Biotechnol. 16: 764-770.
5. Kim, D., Y. K. Park, J. S. Lee, J F. Kim, H. Jeong, B. S. Kim, and C. H. Lee. 2006. Analysis of a prodigiosin biosynthetic gene cluster from the marine bacterium Hahella chejuensis KCTC 2396. J. Mcrobiol. Biotechnol. 16: 1912-1918.
6. Kimura, H., M. Izawa, and Y. Sumino. 1996. Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90. Appl. Microbiol. Biotechnol. 45: 589-596.
7. Kimura, H., H. Miyashita, and Y. Sumino. 1996. Organization and expression in Pseudomonas putida of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90. Appl. Microbiol. Biotechnol. 45: 490-501.
8. Kohli, R. M., J. W. Trauger, D. Schwarzer, M. A. Marahiel, and C. T. Walsh. 2000. Generality of peptide cyclization catalyzed by isolated thioesterase domains of nonribosomal peptide. synthetases. Biochemistry 40: 7099-7108.
9. Liras, P. and J. F. Martiin. 2006. Gene clusters for β-lactam antibiotics and control of their expression: Why have clusters evolved, and from where did they originate? Int. Microbiol. 9: 9-1.
10. Martin, J. F. 1998. New aspects of genes and enzymes for β-lactam antibiotic biosynthesis. Appl. Microbiol. Biotechnol. 50: 1-15.
11. Mofid, M. R., L. O. Essen, and M. A. Marahiel. 2004. Structure-based mutational analysis of the 4'-phosphopantetheinyl transferases sfp from Bacillus subtilis. Biochemistry 43: 4127-4136.
12. Nozaki, Y., K. Okonogi, N. Katayama, H. Ono, S. Harada, M. Kondo, and H. Okazaki. 1984. Cephabacins, new cephem antibiotics of bacterial origin. IV. Antibacterial activities, stability to β-lactamases and mode of action. J. Antibiot. 37: 1555-1565.
13. Park, M. J., J. O. Yon, S. K. Lim, D. D. Y. Ryu, and D. H. Nam. 2004. Biochemical characterization of an ABC transporter gene involved in cephabacin biosynthesis in Lysobacter lactamgenus. J. Microbiol. Biotechnol. 14: 635-638.
14. Ryu, J. K. and D. H. Nam. 1997. Cloning of isopenicillin N synthase gene from Lysobacter lactamgenus. J. Microbiol Biotechnol. 7: 373-377.
15. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
16. Sohn, Y S., D. H. Nam, and D. D. Y. Ryu. 2001. Biosynthetic pathway of cephabacins in Lysobacter lactamgenus: Molecular and biochemical characterization of the upstream region of the gene cluster for engineering of novel antibiotics. Metabol. Eng. 3: 380-382.
17. Tsubotani, S., T. Hida, F. Kasahara, Y. Wada, and S. Harada. 1984. Cephabacins, new cephem antibiotics of bacterial origin. III. Structural determination. J. Antibiot. 37: 1546-1554.
18. Wu, J., K. Kinoshita, C. Khosla, and D. F. Cane. 2004. Biochemical analysis of the substrate specificity of the β-ketoacyl-acyl carier protein synthase domain of module 2 of the erythromycin polyketide synthase. Biochemistry 43: 16301-16310.