A novel genetic selection system for improved enantioselectivity of Bacillus subtilis lipase A

ChemBioChem

Volumn 9, Issue 7, 2008, Pages 1110-1115

  Boersma, Ykelien L ^a   Dröge, Melloney J ^b   van der Sloot, Almer M ^c   Pijning, Tjaard ^a   Cool, Robbert H ^a   Dijkstra, Bauke W ^a   Quax, Wim J ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b ABL BV   (Netherlands)

^c CENTRE FOR GENOMIC REGULATION CRG   (Spain)

Author keywords

Bacillus subtilis lipase A; Directed evolution; Dual selection; Enantioselectivity; Genetic selection

Indexed keywords

1,2 O ISOPROPYLIDENE SN GLYCEROL; ACID LIPASE; GLYCEROL; PHOSPHONIC ACID ESTER; UNCLASSIFIED DRUG; 1,2 O ISOPROPYLIDENE GLYCEROL; 1,2-O-ISOPROPYLIDENE GLYCEROL; ALKENE; ASPARTIC ACID; DRUG DERIVATIVE; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; CATALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENANTIOMER; ENANTIOSELECTIVITY; GENE MUTATION; GENETIC CONSERVATION; GENETIC SELECTION; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN VARIANT; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; CYTOPLASM; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HYDROLYSIS; METABOLISM; METHODOLOGY; MUTATION; STEREOISOMERISM;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

ALKENES; ASPARTIC ACID; BACILLUS SUBTILIS; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE LIBRARY; GLYCEROL; HYDROLYSIS; LIPASE; MUTATION; PERIPLASM; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

EID: 46649102519     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.200700754     Document Type: Article

References (31)

1. U. T. Bornscheuer, K. Buchholz, Eng. Life Sci. 2005, 5, 309-323.
2. A. Schmid, J. S. Dordick, B. Hauer, A. Kiener, M. Wubbolts, B. Witholt, Nature 2001, 409, 258-268.
3. Y. L. Boersma, M. J. Droge, W. J. Quax, FEBS J. 2007, 274, 2181-2195.
4. H. Zhao, K. Chockalingam, Z. Chen, Curr. Opin. Biotechnol. 2002, 13, 104-110.
5. U. T. Bornscheuer, J. Altenbuchner, H. H. Meyer, Bioorg. Med. Chem. 1999, 7, 2169-2173.
6. L. Cipolla, Comb. Chem. High Throughput Screening 2004, 7, 101-114.
7. J. L. Jestin, P. A. Kaminski, J. Biotechnol. 2004, 113, 85-103.
8. J. S. Griffiths, M. Cheriyan, J. B. Corbell, L. Pocivavsek, C. A. Fierke, E. J. Toone, Bioorg. Med. Chem. 2004, 12, 4067-4074.
9. L. B. Evnin, J. R. Vasquez, C. S. Craik, Proc. Natl. Acad. Sci. USA 1990, 87, 6659-6663.
10. S. Y. McLoughlin, C. Jackson, J. W. Liu, D. L. Ollis, Protein Expression Purif. 2005, 41, 433-440.
11. L. G. Otten, C. F. Sio, J. Vrielink, R. H. Cool, W. J. Quax, J. Biol. Chem. 2002, 277, 42121-42127.
12. A. Skandalis, L. P. Encell, L. A. Loeb, Chem. Biol. 1997, 4, 889-898.
13. C. F. Sio, A. M. Riemens, J. M. van der Laan, R. M. D. Verhaert, W. J. Quax, Eur. J. Biochem. 2002, 269, 4495-4504.
14. M. J. Dröge, Y. L. Boersma, G. van Pouderoyen, T. E. Vrenken, C. J. Rüggeberg, M. T. Reetz, B. W. Dijkstra, W. J. Quax, ChemBioChem 2006, 7, 149-157.
15. G. van Pouderoyen, T. Eggert, K.-E. Jaeger, B. W. Dijkstra, J. Mol. Biol. 2001, 309, 215-226.
16. G. Pahel, A. D. Zelenetz, B. M. Tyler, J. Bacteriol. 1978, 133, 139-148.
17. M. J. Dröge, C. J. Rüggeberg, A. M. van der Sloot, J. Schimmel, D. S. Dijkstra, R. M. D. Verhaert, M. T. Reetz, W. J. Quax, J. Biotechnol. 2003, 101, 19-28.
18. M. T. Reetz, C. J. Rüggeberg, Chem. Commun. (Cambridge) 2002, 1428-1429.
19. B. Y. Hwang, J. M. Oh, J. Kim, B. G. Kim, Biotechnol. Lett. 2006, 28, 1181-1185.
20. M. T. Reetz, L. W. Wang, Comb. Chem. High Throughput Screening 2006, 9, 295-299.
21. W. P. Stemmer, Nature 1994, 370, 389-391.
22. M. T. Reetz, L. W. Wang, M. Bocola, Angew. Chem. 2006, 118, 2556; Angew. Chem. Int. Ed. 2006, 45, 2494.
23. A. Kumar, C. Sekharudu, B. Ramakrishnan, C. M. Dupureur, H. Zhu, M. D. Tsai, M. Sundaralingam, Protein Sci. 1994, 3, 2082-2088.
24. D. L. Ollis, E. Cheah, M. Cygler, B. W. Dijkstra, F. Frolow, S. M. Franken, M. Harel, S. J. Remington, I. Silman, J. D. Schrag, J. L. Sussman, K. H. G. Verschueren, A. Goldman, Protein Eng. 1992, 5, 197-211.
25. J. D. Schrag, F. K. Winkler, M. Cygler, J. Biol. Chem. 1992, 267, 4300-4303.
26. V. A. Ilyin, A. Abyzov, C. M. Leslin, Protein Sci. 2004, 13, 1865-1874.
27. M. J. Dröge, Y. L. Boersma, P. G. Braun, R. J. Buining, M. K. Julsing, K. G. Selles, J. M. van Dijl, W. J. Quax, Appl. Environ. Microbiol. 2006, 72, 4589-4594.
28. J. Sambrook, E. F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, 1989.
29. M. J. Dröge, R. Bos, H. J. Woerdenbag, W. J. Quax, J. Sep. Sci. 2003, 26, 1-6.
30. C. S. Chen, Y. Fujimoto, G. Girdaukas, C. J. Sih, J. Am. Chem. Soc. 1982, 104, 7294-7299.
31. C. M. Leslin, A. Abyzov, V. A. Ilyin, Nucleic Acids Res. 2007, 35, D317-D321.