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Volumn 320, Issue 5884, 2008, Pages 1760-1762

Tryptophan-accelerated electron flow through proteins

Author keywords

[No Author keywords available]

Indexed keywords

COPPER; GLYCINE; HISTIDINE; METHIONINE; TRYPTOPHAN; TYROSINE;

EID: 46449093308     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1158241     Document Type: Article
Times cited : (367)

References (34)
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    • Materials and methods are available as supporting material on Science Online.
    • Materials and methods are available as supporting material on Science Online.
  • 23
    • 46449124670 scopus 로고    scopus 로고
    • 124H).
    • 124H).
  • 24
    • 46449117232 scopus 로고    scopus 로고
    • Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
    • Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
  • 28
    • 46449135848 scopus 로고    scopus 로고
    • A. Cannizzo et al., J. Am. Chem. Soc. 130, in press (2008).
    • A. Cannizzo et al., J. Am. Chem. Soc. 130, in press (2008).
  • 29
    • 46449100524 scopus 로고    scopus 로고
    • Although endergonic initial steps can still produce an advantage over single-step tunneling, transport time constants increase by roughly one order of magnitude for each 100 meV increase in -ΔG° Int→z.ast;ML, Hence, tunneling through less stable intermediates, although possible, offers neither a kinetic nor an energetic advantage over a reaction involving exergonic tunneling steps
    • Although endergonic initial steps can still produce an advantage over single-step tunneling, transport time constants increase by roughly one order of magnitude for each 100 meV increase in -ΔG° (Int→z.ast;ML). Hence, tunneling through less stable intermediates, although possible, offers neither a kinetic nor an energetic advantage over a reaction involving exergonic tunneling steps.
  • 32
    • 46449109576 scopus 로고    scopus 로고
    • On the basis of a large body of experimental data, we assume a reorganization energy of 0.8 eV for all ET steps (2, 3, Earlier work has established that the rates of individual tunneling reactions in azurin depend exponentially on the donor-acceptor separation distance, with a decay constant of 1.1 Å-1 (12, rates also depend on driving force [estimated from electrochemical measurements and from the observed *ReII(CO)3(dmp•, H 124, W122)|AzCuI: ReI(CO) 3(dmp•, H124, W122) •+|AzCuI equilibrium constant
    • I equilibrium constant].
  • 34
    • 46449127897 scopus 로고    scopus 로고
    • We thank C. Grǎdinaru, B. Leigh, and J. Miller for assistance in the early stages of this work. Supported by NIH (DK19038 to H.B.G, NSF (CHE-0749997 to B.R.C, and CHE-0533150 to H.B.G. and J.R.W, the Foundation BLANCEFLOR Boncompagni-Ludovisi, née Bildt; STINT, the Swedish Foundation for International Cooperation in Research and Higher Education (MLAA, the Engineering and Physical Sciences Research Council; Queen Mary, University of London; and the Science and Technology Facilities Council (CMSD43, The coordinates of the ReI(CO)3 (dmp)(H124, W 122)|AzCuII crystal structure have been deposited in the Protein Data Bank accession number 2I7O
    • II crystal structure have been deposited in the Protein Data Bank (accession number 2I7O).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.