Design, production, and characterization of recombinant neocarzinostatin apoprotein in Escherichia coli

Journal of Biochemistry

Volumn 131, Issue 5, 2002, Pages 729-738

  Nozaki, Seishiro ^a   Tomioka, Yoshihisa ^a   Hishinuma, Takanori ^a   Inoue, Masayuki ^b   Nagumo, Yoko ^b   Tsuruta, Lilian R ^a   Hayashi, Katsuhiro ^a   Matsumoto, Toshiki ^a   Kato, Yoshinori ^a   Ishiwata, Shunji ^a   Itoh, Kunihiko ^c   Suzuki, Toshio ^c   Hirama, Masahiro ^b   Mizugaki, Michinao ^a  

^a TOHOKU UNIVERSITY HOSPITAL   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

^c AKITA UNIVERSITY HOSPITAL   (Japan)

Author keywords

Ethidium bromide; Fluorescence polarization analysis; Histone; Megaprimer polymerase chain reaction; Neocarzinostatin; Phage display

Indexed keywords

APOPROTEIN; APOZINOSTATIN; DRUG DERIVATIVE; ENEDIYNE; ETHIDIUM; HISTONE; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; ZINOSTATIN; ZINOSTATIN CHROMOPHORE; HISTONE H1; HISTONE H2A; HISTONE H2B; HISTONE H3; HISTONE H4; PHENYLALANINE; TRYPTOPHAN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CATTLE; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS; THYMUS; WESTERN BLOTTING; AMINO ACID SUBSTITUTION; CHROMATOPHORE; DISSOCIATION CONSTANT; FLUORESCENCE; GENE LIBRARY; MOLECULAR CLONING; NONHUMAN; PHAGE DISPLAY; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; APOPROTEINS; BASE SEQUENCE; BLOTTING, WESTERN; CATTLE; ENEDIYNES; ESCHERICHIA COLI; ETHIDIUM; FLUORESCENCE POLARIZATION; HISTONES; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; RECOMBINANT PROTEINS; THYMUS GLAND; ZINOSTATIN;

ESCHERICHIA COLI; NEGIBACTERIA; SYNTHETIC CONSTRUCT;

EID: 0036090311     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003158     Document Type: Article

References (50)

1. Neocarzinostatin, an antitumor antibiotic of high molecular weight: Isolation, physiochemical properties and biological activities
2. Neocarzinostatin: Spectral characterization and separation of a non-protein chromophore
3. Isolation of a non-protein component and a protein component from neocarzinostatin (NCS) and their biological activities
4. The structure of neocarzinostatin chromophore processing a novel bicyclo [7,3,0] dodecadiyne system
5. Absolute configuration of the amino sugar moiety of the neocarzinostatin chromophore
6. Kedar cidin, a new chromoprotein antitumor antibiotic. 1. Taxonomy of producing organism, fermentation and biological-activity
7. Kedarcidin, a new chromoprotein antitumor antibiotic. II. Isolation, purification and physico-chemical properties
8. Kedarcidin chromophore: An enediyne that cleaves DNA in a sequence- specific manner
9. A new macromolecular antitumor antibiotic, C-1027. II. Isolation and physico-chemical properties
10. Some characteristics of DNA strand scission by macromolecular antitumor antibiotic C-1027 containing a novel enediyne chromophore
11. C1027 chromophore, a potent new enediyne antitumor antibiotic, induces sequence-specific double-strand DNA cleavage
12. Maduropeptin, a complex of new macromolecular antitumor antibiotics
13. Maduropeptin: An antitumor chromoprotein with selective protease activity and DNA cleaving properties
14. Nucleotide-specific cleavage and minorgroove interaction of DNA with esperamicin antitumor antibiotics
15. Enediyne-mediated DNA damage in nuclei is modulated at the level of the nucleosome
16. Understanding and exploiting nature's chemical arsenal: The past, present and future of calicheamicin research
17. DNA intercalation and cleavage of an antitumor antibiotic dynemicin that contains anthracycline and enediyne cores
18. Clinical trials of a new antitumor polypeptide: Neocarzinostatin (NCS)
19. Phase I and preliminary phase II study of neocarzinostatin
20. SMANCS and polymer-conjugated macromolecular drugs: Advantage in cancer chemotherapy
21. Crystal structure of neocarzinostatin, an antitumor proteinchromophore complex
22. Selective proteolytic activity of the antitumor agent kedarcidin
23. Reinvestigation of the proteolytic activity of neocarzinostatin
24. Sequential 1H NMR assignment of the complex of aponeocarzinostatin with ethidium bromide and investigation of protein-drug interactions in the chromophore binding site
25. Polarisation de la lumiere de fluorescence vie moyenne des molecules dans letat excite
26. Plasma membrane changes during corpus luteum regression
27. Rapid diffusion immunoassay in a T-sensor
28. Unsaturated fatty acids inhibit transcription of the sterol regulatory element-binding protein-1c (SREBP-1c) gene by antagonizing ligand-dependent activation of the LXR
29. Genotyping single-nucleotide polymorphisms by the invader assay with dual-color fluorescence polarization detection
30. Fluorescence polarization in homogeneous nucleic acid analysis II: 5′-nuclease assay
31. Codon usage tabulated from the international DNA sequence databases
32. PJuFo: A phagemid for display of cDNA libraries on phage surface suitable for selective isolation of clones expressing allergens
33. Cloning Aspergillus fumigatus allergens by the pJuFo filamentous phage display system
34. Detection of heat-stable delta 3,delta 2-enoyl-CoA isomerase in rat liver mitochondria and peroxisomes by immunochemical study using specific antibody
35. The "megaprimer" method of site-directed mutagenesis
36. "Megaprimer" method of PCR-based mutagenesis: The concentration of megaprimer is a critical factor
37. A 3-dimensional model for the CD40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors
38. Protein modeling by E-mail
39. SWISS-MODEL and Swiss-Pdb Viewer: An environment for comparative protein modeling
40. Mechanism of ethidium bromide fluorescence enhancement on binding to nucleic acids
41. Solution structure of the antibiotic neocarzinostatin, a chromophore-protein complex
42. Crystal structure of apo-neocarzinostatin at 0.15-nm resolution
43. Sequential 1H NMR assignments and secondary structure of aponeocarzinostatin in solution
44. Proton NMR studies of apo-neocarzinostatin from Streptomyces carzinostaticus. Sequence-specific assignment and secondary structure
45. Neocarzinostatin: Interaction between the antitumor-active chromophore and the carrier protein
46. Two- and three-dimensional proton NMR studies of apo-neocarzinostatin
47. The seven-stranded beta-barrel structure of apo-neocarzinostatin as compared to the immunoglobulin domain
48. Characterization of secondary structure of neocarzinostatin apoprotein
49. Specific binding of TES-23 antibody to tumour vascular endothelium in mice, rats and human cancer tissue: A novel drug carrier for cancer targeting therapy