Enhanced expression of human cytochrome P450 1A2 by co-expression with human molecular chaperone Hsp70

Toxicology Letters

Volumn 153, Issue 2, 2004, Pages 267-272

  Ahn, Taeho ^a   Yang, Siyoung ^a   Yun, Chul Ho ^b  

^a Chonnam National University   (South Korea)

^b PaiChai University   (South Korea)

Author keywords

ALA; aminolevulinic acid; 3 (3 cholamidopropyl)dimethyl ammonio 1 propanesulfonate; Chaperone; CHAPS; Co expression; CYP or P450; CYP1A2; cytochrome P450; E. coli; Escherichia coli; Hsp70; IPTG; isopropyl D thiogalactopyranoside; PVDF

Indexed keywords

CHAPERONE; CYTOCHROME P450 1A2; HEAT SHOCK PROTEIN 70; RECOMBINANT PROTEIN;

ARTICLE; ESCHERICHIA COLI; GENETIC CODE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION;

CYTOCHROME P-450 CYP1A2; ESCHERICHIA COLI; HSP70 HEAT-SHOCK PROTEINS; HUMANS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 4644245307     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxlet.2004.05.002     Document Type: Article

References (22)

1. Burke M.D., Mayer R.T. Differential effects of phenobarbitone and 3-methylcholanthrene induction on the hepatic microsomal metabolism and cytochrome P450-binding of phenoxazone and a homologous series of its n-alkylehters (alkoxyresorufins). Chem. Biol. Interact. 45:1983;243-258
2. PA (P-4501A2), the phenacetin O-deethylase, is primarily responsible for the hepatic 3-demethylation of caffeine and N-oxidation of carcinogenic arylamines. Proc. Natl. Acad. Sci. USA. 86:1989;7696-7700
3. Distlerath L.M., Reilly P.E., Martin M.V., Davis G.G., Wilkinson G.R., Guengerich F.P. Purification and characterization of the human liver cytochromes P-450 involved in debrisoquine 4-hydroxylation and phenacetin O-deethylation, two prototypes for genetic polymorphism in oxidative drug metabolism. J. Biol. Chem. 260:1985;9057-9067
4. Gething M.J. Molecular chaperones: clasping the prize. Curr. Biol. 6:1996;1573-1576
5. Gillam E.M., Baba T., Kim B.R., Ohmori S., Guengerich F.P. Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme. Arch. Biochem. Biophys. 305:1993;123-131
6. Guengerich, F.P., 1995. Human cytochrome P450 enzymes. In: Ortiz de Montelano, P.R. (Ed.), Cytochrome P450, 2nd ed. Plenum Press, New York, pp. 473-535.
7. Guengerich F.P., Parikh A. Expression of drug-metabolizing enzymes. Curr. Opin. Biotechnol. 8:1997;623-628
8. Hanna I.H., Teiber J.F., Kokones K.L., Hollenberg P.F. Role of the alanine at position 363 of cytochrome P450 2B2 in influencing the NADPH- and hydroperoxide-supported activities. Arch. Biochem. Biophys. 350:1998;324-332
9. Inoue E., Takahashi Y., Imai Y., Kamataki T. Development of bacterial expression system with high yield of CYP3A7, a human fetus-specific form of cytochrome P450. Biochem. Biophys. Res. Commun. 269:2000;623-627
10. Iwata H., Fujita K.-I., Kushida H., Suzuki A., Konno Y., Nakamura K., Fujino A., Kamataki T. High catalytic activity of human cytochrome P450 co-expressed with human NADPH-cytochrome P450 reductase in Escherichia coli. Biochem. Pharmacol. 55:1998;1315-1325
11. Kadlubar F.F., Butler M.A., Kaderlik K.R., Chou H.C., Lang N.P. Polymorphisms for aromatic amine metabolism in humans: relevance for human carcinogenesis. Environ. Health Perspect. 98:1992;69-74
12. Kaminsky L.S., Fasco M.J., Guengerich F.P. Production and application of antibodies to rat liver cytochrome P-450. Meth. Enzylmol. 74:1981;262-272
13. Morimoto, R.I., Tissiéres, A., Georgopoulos, C., 1990. The stress response, functions of the proteins, and perspectives. In: Morimoto, R.I., Tissiéres, A., Georgopoulos, C. (Eds.), Stress Proteins in Biology and Medicine. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 1-36.
14. Omura T., Sato R. The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J. Biol. Chem. 239:1964;2370-2378
15. Parikh A., Guengerich F.P. Expression, purification, and characterization of a catalytically active human cytochrome P450 1A2: rat NADPH-cytochrome P450 fusion protein. Protein Expr. Purif. 9:1997;346-354
16. Pelham, H.R.B., 1990. Functions of the hsp70 protein family: an overview. In: Morimoto, R.I., Tissiéres, A., Georgopoulos, C. (Eds.), Stress Proteins in Biology and Medicine. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 287-299.
17. Pritchard M.P., Ossentian R., Li D.N., Henderson C.J., Burchell B., Wolf R., Friedberg T. A general strategy for the expression of recombinant human cytochrome P450s in Escherichia coli using bacterial signal peptides: expression of CYP3A4, CYP2A6, and CYP2E1. Arch. Biochem. Biophys. 345:1997;342-354
18. Sandhu P., Baba T., Guengerich F.P. Expression of modified cytochrome P450 2C10 (2C9) in Escherichia coli, purification, and reconstitution of catalytic activity. Arch. Biochem. Biophys. 306:1993;443-450
19. Sanger F., Coulson A.R., Barrell B.G., Smith A.J., Roe B.A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J. Mol. Biol. 143:1980;161-178
20. Shimada T., Martin M.V., Pruess-Schwartz D., Marnett L.J., Guengerich F.P. Roles of individual human cytochrome P-450 enzymes in the bioactivation of benzo(a)pyrene, 7,8-dihydroxy-7,8-dihydrobenzo(a)pyrene, and other dihydrodiol derivatives of polycyclic aromatic hydrocarbons. Cancer Res. 49:1989;6304-6312
21. Wrighton S.A., Stevens J.C. The human hepatic cytochromes P450 involved in drug metabolism. Crit. Rev. Toxcol. 22:1992;1-21
22. Yun C.H., Miller G.P., Guengerich F.P. Rate-determining steps in phenacetin oxidations by human cytochrome P450 1A2 and selected mutants. Biochemistry. 39:2000;11319-11329