Targeted disruption of the 3p12 gene, Dutt1/Robo1, predisposes mice to lung adenocarcinomas and lymphomas with methylation of the gene promoter

Cancer Research

Volumn 64, Issue 18, 2004, Pages 6432-6437

  Xian, Jian ^a   Aitchison, Alan ^a   Bobrow, Linda ^b   Corbett, Gerard ^a   Pannell, Richard ^c   Rabbitts, Terence ^c   Rabbitts, Pamela ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b ADDENBROOKE S HOSPITAL   (United Kingdom)

^c MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DUTT1 ROBO1 PROTEIN; GENE PRODUCT; UNCLASSIFIED DRUG;

ALLELE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BREAST TUMOR; BRONCHUS MUCOSA; CANCER SUSCEPTIBILITY; CARCINOGENESIS; CHROMOSOME 3P; CONTROLLED STUDY; DROSOPHILA; DUTT1 ROBO1 GENE; EPITHELIUM HYPERPLASIA; GENE; GENE DELETION; GENE DISRUPTION; GENE INACTIVATION; GENE LOCATION; HETEROZYGOSITY LOSS; HOMOZYGOSITY; IMMUNOHISTOCHEMISTRY; LUNG ADENOCARCINOMA; LUNG DEVELOPMENT; LYMPHOMA; METHYLATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; ROUNDABOUT GENE; TUMOR SUPPRESSOR GENE; WESTERN BLOTTING;

ADENOCARCINOMA; ALLELES; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHROMOSOMES; DNA METHYLATION; GENE DELETION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE SILENCING; GENES, TUMOR SUPPRESSOR; GENETIC PREDISPOSITION TO DISEASE; LUNG NEOPLASMS; LYMPHOMA; MICE; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; NERVE TISSUE PROTEINS; PROMOTER REGIONS (GENETICS); RECEPTORS, IMMUNOLOGIC; TUMOR SUPPRESSOR PROTEINS;

EID: 4644245090     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: 10.1158/0008-5472.CAN-04-2561     Document Type: Article

References (24)

1. Albertson DG, Collins C, McCormick F, Gray JW. Chromosome aberrations in solid turnouts. Nat Genet 2003;34:369-76.
2. Zabarovsky ER, Lerman MI, Minna JD. Tumour suppressor genes on chromosome 3p involved in the pathogenesis of lung and other cancers. Oncogene 2002;21:6915-35.
3. Wistuba II, Behrens C, Virmani AK, et al. High resolution chromosome 3p allelo-typing of human lung cancer and preneoplastic/preinvasive bronchial epithelium reveals multiple, discontinuous sites of 3p allele loss and three regions of frequent breakpoints. Cancer Res 2000;60:1949-60.
4. Lerman MI, Minna JD. The 630kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes. Cancer Res 2000;60:6116-33.
5. Huebner K. Tumor suppressors on 3p: a neoclassic quartet. Proc Natl Acad Sci USA 2001;98:14763-5.
6. Pfeifer GP, Yoon JH, Liu L, et al. Methylation of the RASSF1A gene in human cancers. Biol Chem 2002;383:907-14.
7. Sundaresan V, Chung G, Heppell-Parton A, et al. Homozygous deletions at 3p12 in breast and lung cancer. Oncogene 1998;17:1723-9.
8. Kidd T, Brose K, Mitchell KJ, et al. Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors. Cell 1998;92:205-15.
9. Rao Y, Wong K, Ward M, Jurgensen C, Wu JY. Neuronal migration and molecular conservation with leukocyte chemotaxis. Genes Dev 2002;16:2973-84.
10. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell 2000;100:57-70.
11. Dallol A, Forgacs E, Martinez A, et al. Tumour specific promoter region methylation of the human homologue of the Drosophila Roundabout gene DUTT1 (ROBO1) in human cancers. Oncogene 2002;21:3020-8.
12. Baylin SB, Herman JG. Promoter hypermethylation: can this change alone ever designate true tumor suppressor gene function? J Natl Cancer Inst (Bethesda) 2001;93:664-5.
13. Xian J, Clark KJ, Fordham R, et al. Inadequate lung development and bronchial hyperplasia in mice with a targeted deletion in the Dutt1/Robo1 gene. Proc Natl Acad Sci USA 2001;98:15062-6.
14. Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 1996;93:9821-6.
15. Clark K, Hammond E, Rabbitts P. Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development. FEBS Lett 2002;523:12-6.
16. Tulverson DA, Jacks T. Modelling lung cancer in mice: similarities and shortcoming. Oncogene 1999;18:5318-24.
17. Wales MM, Biel MA, el Deiry W, et al. p53 activates expression of HIC-1, a new candidate tumour suppressor gene on 17p13.3. Nat Med 1995;1:570-7.
18. Chen WY, Zeng X, Carter MG, et al. Heterozygous disruption of Hic1 predisposes mice to a gender-dependent spectrum of malignant tumours. Nat Genet 2003;33:197-202.
19. Sundaresan V, Roberts I, Bateman A, et al. The DUTT1 gene, a novel NCAM family member, is expressed in developing murine neural tissues and has an unusually broad pattern of expression. Mol Cell Neurosci 1998;11:29-35.
20. Derynck R, Akhurst RJ, Balmain A. TGF-β signalling in tumour suppression and cancer progression. Nat Genet 2001;29:117-29.
21. Graff JR, Gabrielson E, Fujii H, Baylin SB, Herman JG. Methylation patterns of the E-cadherin 5′ CpG island are unstable and reflect the dynamic, heterogeneous loss of E-cadherin expression during metastatic progression. J Biol Chem 2000;275:2727-32.
22. Dallol A, Da Silva NF, Viacava P, et al. A human homologue of the Drosoplila Slit2 gene has tumor suppressor activity and is frequently inactivated in lung and breast cancers. Cancer Res 2002;62:5874-80.
23. Dallol A, Morton D, Maher ER, Latif F. SLIT2 axon guidance molecule is frequently inactivated in colorectal cancer and suppresses growth of colorectal carcinoma cells. Cancer Res 2003;63:1054-8.
24. Wang B, Xiao Y, Ding B, et al. Induction of tumour angiogenesis by Slit-Robo signalling and inhibition of cancer growth by blocking Robo activity. Cancer Cell 2003;4:19-29.