A quick and reliable method for monitoring gene expression in actinomycetes

Journal of Biotechnology

Volumn 135, Issue 3, 2008, Pages 262-265

  Chevillotte, Meike ^a   Menges, Rainer ^a   Muth, Günther ^a   Wohlleben, Wolfgang ^a   Stegmann, Evi ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Amycolatopsis balhimycina; Balhimycin; egfp; Fluorescence

Indexed keywords

COMPUTER NETWORKS; GENE EXPRESSION; HEALTH; METROPOLITAN AREA NETWORKS; NETWORK PROTOCOLS;

ENVIRONMENTAL FACTORS; GLYCOPEPTIDE; REGULATORY NETWORKS; RELIABLE METHOD; TRANSCRIPTIONAL REGULATORS; VANCOMYCIN;

ANTIBIOTICS;

BALHIMYCIN;

ACTINOBACTERIA; ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; EGFP GENE; FLUORESCENCE; GENE; GENE EXPRESSION; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION;

ACTINOBACTERIA; BIOTECHNOLOGY; DNA, BACTERIAL; FLUORESCENCE; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; OPERON; PROMOTER REGIONS (GENETICS); REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TIME FACTORS; VANCOMYCIN;

ACTINOBACTERIA (CLASS); AMYCOLATOPSIS BALHIMYCINA;

EID: 45449114133     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.04.008     Document Type: Article

References (20)

1. Bibb M.J., Janssen G.R., and Ward J.M. Cloning and analysis of the promoter region of the erythromycin-resistance gene (ermE) of Streptomyces erythraeus. Gene 38 (1985) 215-226
2. Bibb M.J., White J., Ward J.M., and Janssen G.R. The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythrea is translated in the absence of a conventional ribosome binding site. Mol. Microbiol. 14 (1994) 533-545
3. Bibb M.J. Regulation of secondary metabolism in streptomycetes. Curr. Opin. Microbiol. 8 (2005) 208-215
4. Bongaerts R.J.M., Hautefort I., Sidebotham J.M., and Hinton J.C.D. Green fluorescent protein as a marker for conditional gene expression in bacterial cells. Methods Enzymol. 358 (2002) 43-66
5. Chater K.F. Regulation of sporulation in Streptomyces coelicolor A3(2): a checkpoint multiplex?. Curr. Opin. Microbiol. 4 (2001) 667-673
6. Kataoka M., Kosono S., and Tsujimoto G. Spatial and temporal regulation of protein expression by bldA within a Streptomyces lividans colony. FEBS Lett. 462 (1999) 425-429
7. Fink D., Weissschuh N., Reuther J., Wohlleben W., and Engels A. Two transcriptional regulators GlnR and GlnRII are involved in regulation of nitrogen metabolism in Streptomyces coelicolor A3(2). Mol. Microbiol. 46 (2002) 331-347
8. Menges R., Muth G., Wohlleben W., and Stegmann E. The ABC transporter Tba of Amycolatopsis balhimycina is required for efficient export of the glycopeptide antibiotic balhimycin. Appl. Microbiol. Biotechnol. 77 (2007) 125-134
9. Minas W., Bailey J.E., and Duetz W. Streptomycetes in microcultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format. Antonie Leeuwenhoek 78 (2000) 297-305
10. Nadkarni S.R., Patel M.V., Chatterjee S., Vijayakumar E.K.S., Desikan K.R., Blumbach J., and Ganguli B.N. Balhimycin, a new glycopeptide antibiotic produced by Amycolatopsis sp. Y-86, 21022: taxonomy, production, isolation and biological activity. J. Antibiot. 47 (1994) 334-341
11. Nebe-von-Caron G., Stephens C.J., Hewitt C.J., Powell J.R., and Badley R.A. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J. Microbiol. Methods 42 (2000) 97-114
12. Pelzer S., Reichert W., Huppert M., Heckmann D., and Wohlleben W. Cloning and analysis of a peptide synthetase gene of the balhimycin producer Amycolatopsis mediterranei DSM5908 and development of a gene disruption/replacement system. J. Biotechnol. 56 (1997) 115-128
13. Pelzer S., Süßmuth R.D., Heckmann D., Recktenwald J., Huber P., Jung G., and Wohlleben W. Identification and analysis of the balhimycin biosynthetic gene cluster and its use for manipulating glycopeptide biosynthesis in Amycolatopsis mediterranei DSM5908. Antimicrob. Agents Chemother. 43 (1999) 1565-1573
14. Rebets Y.V., Ostash B.O., Fukuhara M., and Nakamura T. Expression of the regulatory protein LndI for landomycin E production in Streptomyces globisporus 1912 is controlled by the availability of tRNA for the rare UUA codon. FEMS Microbiol. Lett. 256 (2006) 30-37
15. Shawky R.M., Puk O., Wietzorrek A., Pelzer S., Takano E., Wohlleben W., and Stegmann E. The border sequence of the balhimycin biosynthesis gene cluster from Amycolatopsis balhimycina contains bbr, encoding a StrR-like pathway-specific regulator. J. Mol. Microbiol. Biotechnol. 13 (2007) 76-88
16. Stackebrandt E., Rainey F.A., and Ward-Rainey N.L. Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int. J. Syst. Bacteriol. 47 (1997) 479-491
17. Sun J., Kelemen G.H., Fernández-Abalos J.M., and Bibb M.J. Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2). Microbiology 145 (1999) 2221-2227
18. Valdivia R.H., and Falkow S. Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. Mol. Microbiol. 22 (1996) 367-378
19. Vives-Rego J., Lebaron P., and Nebe-von Caron G. Current and future applications of flow cytometry in aquatic microbiology. FEMS Microbiol. Rev. 24 (2000) 429-448
20. van Wezel G.P., Krabben P., Traag B.A., Keijser B.J., Kerste R., Vijgenboom E., Heijnen J.J., and Kraal B. Unlocking Streptomyces spp. for use as sustainable industrial production platforms by morphological engineering. Appl. Environ. Microbiol. 72 (2006) 5283-5288