Superparamagnetic cation-exchange adsorbents for bioproduct recovery from crude process liquors by high-gradient magnetic fishing

Separation Science and Technology

Volumn 39, Issue 12, 2004, Pages 2891-2914

  Heebøll Nielsen, Anders ^a   Justesen, Sune F L ^b   Hobley, Timothy J ^a   Thomas, Owen R T ^c  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Downstream processing; HGMF; Primary capture; Purification

Indexed keywords

ADSORBENTS; ENZYMES; FRACTIONATION; ION EXCHANGE; OLIGOMERS; PARTICLES (PARTICULATE MATTER); PURIFICATION;

CATION-EXCHANGE ADSORBENTS; MAGNETIC FISHING; SUPERMAGNETISM;

MAGNETIC SEPARATION;

ADSORBENT; EPICHLOROHYDRIN; GLUTARALDEHYDE; LACTOPEROXIDASE; LYSOZYME; PROTEIN; SULFITE;

ADSORPTION; ION EXCHANGE; LIQUID-SOLID SEPARATION; PURIFICATION;

CATION EXCHANGE; CONCENTRATION (PARAMETERS); CONFERENCE PAPER; FRACTIONATION; HIGH GRADIENT MAGNETIC FISHING; LIQUID CHROMATOGRAPHY; MAGNETIC SEPARATION; PROCESS DESIGN; PROCESS MONITORING; PROCESS OPTIMIZATION; PRODUCT RECOVERY; PURIFICATION; SUPERPARAMAGNETIC CATION EXCHANGE; SURFACE PROPERTY; SWEET BOVINE WHEY; WHEY;

EID: 4544263240     PISSN: 01496395     EISSN: None     Source Type: Journal    
DOI: 10.1081/SS-200028791     Document Type: Conference Paper

References (24)

1. Hubbuch, J.J.; Matthiesen, D.B.; Hobley, T.J.; Thomas, O.R.T. High gradient magnetic separation versus expanded bed adsorption: a first principle comparison. Bioseparation 2001, 10, 99-112.
2. O'Brien, S.M.; Thomas, O.R.T.; Dunnill, P. Non-porous magnetic chelator supports for protein recovery by immobilised metal affinity adsorption. J. Biotechnol. 1996, 50, 13-26.
3. O'Brien, S.M.; Sloane, R.P.; Thomas, O.R.T.; Dunnill, P. Characterisation of non-porous magnetic chelator supports and their use to recover polyhistidine-Tailed T4 lysozyme from a crude. E. coli. extract. J. Biotechnol. 1997, 54, 53-67.
4. Zulqarnain, K. Scale-up of Affinity Based Separation Based on Magnetic Support Particles; University College London: London, 1999; Ph. D. Thesis.
5. Hubbuch, J.J.; Thomas, O.R.T. High-gradient magnetic affinity separation of trypsin from porcine pancreas. Biotechnol. Bioeng. 2002, 79, 301-313.
6. Heebøll-Nielsen, A.; Dalkiær, M.; Hubbuch, J.J.; Thomas, O.R.T. Superparamagnetic adsorbents for high-gradient magnetic fishing of lectins out of legume extracts. Biotechnol. Bioeng. 2004, 87, 311-323.
7. Xue, B.; Sun, Y. Fabrication and characterization of a rigid magnetic matrix for protein adsorption. J. Chromatogr. A 2002, 947, 185-193.
8. Geberding, S.J.; Byers, C.H. Preparative ion-exchange chromatography of proteins from dairy whey. J. Chromatogr. A 1998, 808, 141-151.
9. Ekstrand, B. Antimicrobial factors in milk-A review. Food Biotechnol. 1989, 3, 105-126.
10. Wolfson, L.M.; Sumner, S.S. Antibacterial activity of the lactoperoxidase system: a review. J. Food Prot. 1993, 56, 887-892.
11. McIntosh, G.H.; Royle, P.J.; Leu, R.K.L.; Regester, G.O.; Johnson, M.A.; Grinsted, R.L.; Kenward, R.S.; Smithers, G.W. Whey proteins as functional food ingredients. Int. Dairy J. 1998, 8, 425-434.
12. Shimazaki, K. Lactoferrin: a marvellous protein in milk. Anim. Sci. J. 2000, 71, 329-347.
13. Vasavada, P.C.; Cousin, M.A. Dairy microbiology and safety. In Dairy Science and Technology Handbook; Hui, Y.H., Ed.; 2 Product Manufacturing; VCH Publishers Ltd.: Cambridge, UK, 1993.
14. Thömmes, J. Fluidized bed adsorption as a primary recovery step in protein purification. Adv. Biochem. Eng. Biotechnol. 1997, 58, 185-230.
15. Heebøll-Nielsen, A.; Choe, W.S.; Middelberg, A.P.J.; Thomas, O.R.T. Efficient inclusion body processing using chemical extraction and high gradient magnetic fishing. Biotechnol. Progr. 2003, 19, 887-898.
16. Hermanson, G.T.; Mallia, A.K.; Smith, P.K. Immobilized Affinity Ligand Techniques; Academic Press: London, UK, 1992.
17. Monsan, P.; Puzo, G.; Mazarguil, H. Étude du mécanisme d'établissement des liaisons glutaraldéhyde-protéines. Biochimie 1975, 57, 1281-1292.
18. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
19. Hoffmann, C.; Franzreb, M.; Höll, W.H. A novel high gradient magnetic separator (HGMS) design for biotech applications. IEEE Trans. Appl. Superconduct. 2002, 12, 963-966.
20. Yoneda, G.S.; Griffin, M.T.; Carlyle, D.W. A kinetic study of the reaction between sulfite ion and propylene oxide. J. Org. Chem. 1975, 40, 375-377.
21. Royappa, A.T. On the copolymerisation of epichlorohydrin and glycidol. Int. J. Appl. Poly. Sci. 1997, 65, 1897-1904.
22. Hoffmann, C. Einsatz Magnetischer Separationsverfahren zur Biotechnologischen Produktaufarbeitung; Forschungszentrum Karlsruhe: Germany, 2002; Ph.D. Thesis.
23. Haynes, C.A.; Sliwinsky, E.; Norde, W. Structural and electrostatic properties of globular proteins at a polystyrene-water interface. J. Colloid Interf. Sci. 1994, 164, 394-409.
24. Hubbuch, J.J. Development of Adsorptive Separation Systems for Recovery of Proteins from Crude Bioprocess Liquors; The Technical University of Denmark: Denmark, 2001; Ph. D. Thesis.