High-gradient magnetic affinity separation of trypsin from porcine pancreatin

Biotechnology and Bioengineering

Volumn 79, Issue 3, 2002, Pages 301-313

  Hubbuch, Jürgen J ^a,b   Thomas, Owen R T ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Affinity separation; Batch adsorption; High gradient magnetic separation; Primary capture; Trypsin

Indexed keywords

ACTIVATION ANALYSIS; ENZYMES; MACROMOLECULES; MAGNETIC SEPARATION; OPTIMIZATION; PARTICLE SIZE ANALYSIS; PURIFICATION; SUPERPARAMAGNETISM;

CRUDE PORCINE PANCREATIN FEEDSTOCK;

BIOTECHNOLOGY;

ADSORBENT; AMYLASE; BENZAMIDINE; PANCREATIN; TRYPSIN;

ADSORPTION; ARTICLE; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; MAGNETIC FIELD; MAGNETISM; NONHUMAN; PARTICLE SIZE; PROTEIN PURIFICATION; RECYCLING; SWINE;

ADSORPTION; ANIMALS; BENZAMIDINES; CATTLE; MACROMOLECULAR SUBSTANCES; MAGNETICS; MODELS, CHEMICAL; PANCREATIN; PILOT PROJECTS; QUALITY CONTROL; SENSITIVITY AND SPECIFICITY; SWINE; TRYPSIN;

SUIDAE;

EID: 0037026235     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10285     Document Type: Article

References (57)

1. Anspach FB, Wirth HJ, Unger KK, Stanton P, Davies JR, Hearn MTW. 1989. High performance liquid chromatography with phenylboronic acid, benzamidine, tri-L-alanine and concanavilin A immobilised on 3-isothiocyanatopropyl-triethoxysilane-activated nonporous monodisperse silicas. Anal Biochem 179:171-181.
2. Barnfield Frej AK, Hjorth R, Hammarström A. 1994. Pilot scale recovery of recombinant annexin V from unclarified Escherichia coli homogenate using expanded bed adsorption. Biotechnol Bioeng 44:922-929.
3. Bui QT, Pankhurst QA, Zulquarnain K. 1998. Inter-particle interactions in biocompatible magnetic fluids. IEEE Trans Mag 34(4):2117-2119.
4. Carlsen M. 1994. α-Amylase production in Aspergillus oryzae. Ph.D. Thesis, Technical University of Denmark.
5. Cornell RM, Schwertmann U. 1996. The iron oxides. Weinheim, Germany: VCH Verlagsgesellschaft mbH.
6. Dorman JL, Fiorani D. 1992. Magnetic properties of fine particles. Amsterdam: Elsevier North Holland.
7. Erlanger BF, Kokowsky N, Cohen W. 1961. The preparation and properties of two new chromogenic substrates for trypsin. Arch Biochem Biophys 95:271-278.
8. Eveleigh JW. 1978. Techniques and instrumentation for preparative immunosorbent separations. J Chromatogr 159:129-145.
9. Groman EV, Wilchek M. 1987. Recent developments in affinity chromatography supports. Trends Biotechnol 5:220-224.
10. Halling PJ, Dunnill P. 1979a. Recovery of free enzymes from product liquors by bio-affinity adsorption: Trypsin binding by immobilised soybean inhibitor. Eur J Appl Microb Technol 6:195-205.
11. Halling PJ, Dunnill P. 1979b. Improved nonporous magnetic supports for immobilised enzymes. Biotechnol Bioeng 21:396-416.
12. Halling PJ, Dunnill P. 1980. Magnetic supports for immobilized enzymes and bioaffinity adsorbents. Enzyme Microb Technol 2:2- 10.
13. Hannson M, Stål S, Hjorth R, Uhlen M, Moks T. 1994. Single step recovery of a secreted recombinant protein by EBA. Bio/Technology 12:285-288.
14. Hermanson GT, Malia AK, Smith PK. 1992. Immobilised affinity ligand techniques. London: Academic Press.
15. Hubbuch JJ. 2001. Development of adsorptive separation systems for recovery of proteins from crude bioprocess liquors. Ph.D. Thesis, Technical University of Denmark.
16. Hubbuch JJ, Matthiesen D, Hobley TJ, Thomas ORT. 2001. High-gradient magnetic separation versus expanded bed adsorption: A first principle comparison. Bioseparation 10:99-112.
17. Kasumasa Y, Muehiro N, Takao O, Akinori S. 1996. Process for purifying recombinant human serum albumin. European patent, EP0699687.
18. Kobayashi H, Matsunaga T. 1991. Amino-silane modified super-paramagnetic particles with surface-immobilized enzyme. J Col Inter Sci 141(2):505-511.
19. Laemmli U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 227:680-685.
20. Langmuir I. 1918. The adsorption of gases on plane surfaces of glass, mica and platinum. J Am Chem Soc 40:1361-1403.
21. Leitermann WJ. 1985. Untersuchung von Partikeltrajectorien in HGMS-Modellfiltern transversaler Anordnung und des Filterverhaltens bei extrem hohen Durchflussraten für Teilchen mit Radien im 1 μm Bereich. Ph.D. Thesis, University of Regensburg, Germany.
22. McCreath GE, Chase HA, Owen RO, Lowe CR. 1995. Expanded bed affinity chromatography of dehydrogenases from baker's yeast using dye-ligand perfluoropolymer supports. Biotechnol Bioeng 48:341-354.
23. Misawa T, Hashimoto K, Shimodaira S. 1974. The mechanism of formation of iron oxide and oxyhydroxides in aqueous solutions at room temperature. Corros Sci 14:131-149.
24. Moffat G, Williams RA, Webb C, Stirling R. 1994. Selective separations in environmental and industrial processes using magnetic carrier technology. Min Eng 7(8):1039-1056.
25. Molday RS, Molday LL. 1984. Separation of cells labelled with immunospecific iron dextran microspheres using high-gradient magnetic chromatography. FEBS Lett 170(2):232-238.
26. Morgan PE. 1996. Non-porous pseudo affinity supports for the recovery of antibodies. Ph.D. Thesis, University College London, UK.
27. Morgan PEM, Thomas ORT, Dunnill P, Sheppard AJ, Slater NKH. 1996. Polyvinyl alcohol-coated perfluorcarbon supports for metal chelating affinity separation of a monoclonal antibody. J Molec Recognit 9:394-400.
28. Mørup S, Tronc E. 1994. Superparamagnetic relaxation of weakly interacting particles. Phys Rev Lett 72(20):3278-3281.
29. Munro PA, Dunnill P, Lilly MD. 1977. Nonporous magnetic materials as enzyme supports: studies with immobilised chymotrypsin. Biotechnol Bioeng 19:101-124.
30. Nesset JE, Finch JA. 1980. A loading equation for HGMS and application in identifying the fine size limit of recovery. In: Somasundaran P, editor. Fine particle processing, Vol. 2. New York: AIME. pp 1217-1241.
31. Nesset JE, Todd I, Hollingworth M, Finch JA. 1980. A loading equation for high-gradient magnetic separation. IEEE Trans Magn 16(5):833-835.
32. O'Brien SM, Thomas ORT, Dunnill P. 1996. Non-porous magnetic chelator supports for protein recovery by immobiliesd metal affinity adsorption. J Biotechnol 50:13-25.
33. O'Brien SM, Sloane RP, Thomas ORT, Dunnill P. 1997. Characterisation of non-porous magnetic chelator supports and their use to recover polyhistidine-tailed T4 lysozyme from a crude E. coli extract. J Biotechnol 54:53-67.
34. Naviroj S, Culler SR, Koenig JL, Ishida H. 1984. Structure and adsorption characteristics of silane coupling agents on silica and E-glass fiber: Dependence on pH. J Coll Inter Sci 97(2):308-317.
35. Parker MR, van Kleef RPAR, Myron HW, Wyder P. 1982. Study of particle behaviour in high field magnetic flocculation. IEEE Trans Magn 18:1647-1649.
36. Plueddemann EP. 1991. Silane coupling agents, 2nd edition. Plenum Press New York and London: Plenum Press.
37. Scatchard G. 1949. The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51:660-672.
38. Schluep T, Cooney CL. 1998. Purification of plasmids by triplex affinity interaction. Nucleic Acids Res 26(19):4524-4528.
39. Schrader ME, Block A. 1971. Tracer study of kinetics and mechanism of hydrolytically induced interfacial failure. J Polym Sci C 34:281-291.
40. Schwertmann U, Cornell RM. 1991. Iron oxides in the Laboratory. Preparation and characterisation. VCH Verlagsgesellschaft mbH, Weinheim, Germany.
41. Schwertmann U, Fechter H. 1994. The formation of green rust and its transformation to lepidocrocite. Clay Miner 29:87-92.
42. Setchell CH. 1985. Magnetic separations in biotechnology-A review. J Chem Technol Biotechnol 35B:175-182.
43. Spalding BJ. 1991. Downstream processing: Key to slashing production costs 100-fold. Bio/Technology 9:229-233.
44. Shinkai M, Honda H, Kobayashi T. 1991. Preparation of fine magnetic particles and application for enzyme immobilisation. Biocat 5:61-69.
45. Svoboda J. 1981. A theoretical approach to the magnetic flocculation of weakly magnetic minerals. Int J Min Proc 3:377-390.
46. Svoboda J. 1987. Magnetic methods for the treatment of minerals. Amsterdam: Elsevier.
47. Thömmes J. 1997. Fluidized bed adsorption as a primary recovery step in protein purification. Adv Biochem Eng Biotechnol 58:185-230.
48. Tsouris C, Scott TC, Harris MT. 1995. Para- and dia-magnetic particle flocculation in a magnetic field. Sep Sci Technol 30:1407-1419.
49. Uchiyama S, Kumazanu M, Korinobu S, Takayasu M. 1977. Magnetic particle build-up process in parallel stream type HGMS filters. IEEE Trans Magn 13(5):1490-1492.
50. Wang Y, Pugh RJ, Forssberg E. 1994. The influence of interparticle surface forces on the coagulation of weakly magnetic mineral ultrafines in a magnetic field. Coll Surf A: Physicochem Eng Asp 90:117-133.
51. Watson JHP. 1973. Magnetic filtration. J Appl Phys 44:4209-4213.
52. Watson JHP. 1994. Selectivity and mechanical retention in the magnetic separation of polydisperse, mixed mineral particle systems-Part 1. Min Eng 7:769-791.
53. Weetall HH. 1993. Preparation of immobilised proteins covalently coupled through silane coupling agents to inorganic supports. Appl Biochem Biotechnol 41:157-188.
54. Whitehead RA, Chagnon MS, Groman EV, Josephson L. 1985. U.S. Patent number 4,554,088, November 19.
55. Williams RA, Pieters BR, Webb C. 1992. Process engineering of magnetic immobilization systems for enzyme reactors and effluent treatment. In: Williams RA, editor. Colloid and surface engineering: Applications in the process industries. Oxford: Butterworth-Heinemann, pp 287-345.
56. Wils P, Escriou V, Warnery A, Lacroix F, Lagneaux D, Olliver M, Crouzet J, Mayaux J-F, Scherman D. 1997. Efficient purification of plasmid DNA for gene transfer using triple-helix affinity chromatography. Gene Therapy 4:323-330.
57. Zulqarnain K. 1999. Scale-up of affinity separation based on magnetic support particles, Ph.D. Thesis. University College London, UK.